Effect of kenaf seed oil from different ways of extraction towards ovarian cancer cells

Abstract

Kenaf (Hibiscus cannabinus) from the family of Malvaceae is a valuable fibre plant native to India and Africa. Kenaf is composed of various active components including tannins, saponins, polyphenolics, alkaloids, essential oils and steroids. It has been used to treat bruises, bilious conditions, fever and puerperium. Nevertheless, the anti-cancer properties of kenaf seed oil have not yet been investigated. In this study, kenaf seed oils obtained by Sonication, Soxhlet and supercritical carbon dioxide fluid extraction (SFE) with 9 different combinations of pressure (bars) and temperature (°C) (200/40, 200/60, 200/80, 400/40, 400/60, 400/80, 600/40, 600/60 and 600/80) were investigated for the cytotoxicities. All the oils were cytotoxic towards ovarian cancer (CaOV3) and colon cancer (HT29) cell lines in a dose dependent manner as detected by using the MTT assay and trypan blue dye exclusion method. Oil from Sonication was the most cytotoxic towards CaOV3 cell line. Treated cells exhibited characteristics of apoptosis such as chromatin condensation and nuclear fragmentation. In conclusion, kenaf seed oils from the three extractions were cytotoxic towards CaOV3 cell line in a dose-dependent manner possibly via the induction of apoptosis. In considering the safety of the product, SFE technology is a better alternative extraction method that is suitable in kenaf seed oil extraction.

Chemistry, phytotechnology, pharmacology and nutraceutical functions of kenaf ( Hibiscus cannabinus L.) and roselle ( Hibiscus sabdariffa L.) seed oil: An overview

“…The high extraction yield for hexane may be due to the high oil content in kenaf seed (Conkerton et al, 1995), ranging from 21.4% to 26.4% (Mohamed et al, 1995;Chan and Ismail, 2009;Nyam et al, 2009). Yazan et al (2011) extracted kenaf seed oils by sonication, soxhlet and SFE methods with different combinations of pressure and temperature and the obtained oils showed promising cytotoxic properties towards ovarian cancer cell lines in a dose dependent manner. The SFE technology was recommended as a superior and safe alternative method that is suitable in kenaf seed oil extraction (Wang and Weller, 2006;Chan and Ismail, 2009;Yazan et al, 2011;Ghafar et al, 2012).…”

“…However, to overcome the toxicity and safety issues modern phyto-extraction techniques like sonication, soxhlet, supercritical carbon dioxide fluid extraction (SFE) etc. have been extensively utilized for extracting kenaf seed oil (Wang and Weller, 2006;Chan and Ismail, 2009;Foo et al, 2011;Mariod et al, 2011;Yazan et al, 2011;Ghafar et al, 2013;Ng et al, 2013). In recent time, soxhlet and SFE methods have extensively been used for extraction of kenaf seed oil with enriched fatty acids and antioxidants (Chan and Ismail, 2009;Nyam et al, 2010;Mariod et al, 2011;Ng et al, 2013).…”

Use of selenium fertilizers for production of Se‐enriched Kenaf (Hibiscus cannabinus): Effect on Se concentration and plant productivity

“…The use of kenaf as a feed crop has been successfully tested on beef cattle and small ruminants, and resulted in high percentage of proteins, nonfatty solids, and total solids in the milk (Xiccato et al, 1998;Lopez et al, 2006). Recent studies show that kenaf seed-oil enhances apoptosis towards ovarian cancer cells (Yazan et al, 2011). …”

Effect of moisture on the physical properties of three varieties of kenaf seeds

“…Kenaf (Hibiscus cannabinus L.) from the family Malvaceae is a common warm season annual fiber plant native to India and Africa (Yazan et al 2011). Although kenaf is a tropical plant, today its cultivars are well adapted to a wide geographical and climatic range (Danalatos and Archontoulis 2010).…”

“…Kenaf seeds contain 9.6 % moisture, 6.4 % ash, 20.4 % oil, 21.4 % nitrogenous matter and 12.9 % crude fiber and can also be used as livestock due to their high protein content (more than 20 %) (Nyam et al 2009). Kenaf seed oils contain healthy compounds: α-linolenic acid, β-sitosterol and vitamin E, and they have positive effects on human health such as anticancer, antioxidant and lipid-lowering cholesterol properties (Yazan et al 2011).…”