Effect of Laser Acupuncture on Disuse Osteoarthritis: An Ultrasound Biomicroscopic Study of Patellar Articular Cartilage in Rats

Abstract

To investigate the effect of laser acupuncture (LA) on disuse changes in articular cartilage using ultrasound biomicroscopy (UBM), Eighteen rats were randomly divided into the control group (C), the tail-suspended group (T), and the tail-suspended with LA treatment group (L). During 28-day suspension period, group L were treated with LA at acupoints on the left hindlimb while group T had a sham treatment. Ultrasound roughness index (URI), integrated reflection coefficient (IRC), integrated backscatter coefficient (IBC), cartilage thickness, and ultrasonographic score (US) of articular cartilage at patella were measured by using an ultrasound biomicroscopy system (UBS). Compared with the group C, URI significantly (P < 0.01) increased by 60.9% in group T, increased by 38.1% in group L. In addition, unloading induced a significant cartilage thinning (P < 0.05) in group T, whereas cartilage thickness in group L was 140.22 ± 19.61 μm reaching the level of the control group (147.00 ± 23.99 μm). There was no significant difference in IRC, IBC, and US among the three groups. LA therapy could help to retain the quality of articular cartilage which was subjected to unloading. LA would be a simple and safe nonpharmacological countermeasure for unloading-induced osteoarthritis. The UBM system has potential to be a sensitive, specific tool for quantitative assessment of articular cartilage.

Early Detection of Tibial Cartilage Degradation and Cancellous Bone Loss in an Ovariectomized Rat Model

“…The cartilage results of this study are similar to those of previous study showing that disuse-induced significant changes in the cartilage tissue [ 25 ]. However, disuse simultaneously induced cartilage degradation and bone loss [ 26 ].…”

The Application of Laser Acupuncture in Animal Experiments: A Narrative Review of Biological Aspects

“…Guo et al [ 64 ] proved that laser irradiation on KI-1 and ST-36 could inhibit bone loss in rats caused by skeletal unloading via a systemic regulation. Besides, in their further investigation [ 65 ], they suggested that unloading-induced cartilage degeneration in rats can be reversed by the same laser therapy. KI-3 and BL-60 are located on either side of the Achilles tendon and are a common acupoint combination for the treatment of pain in the lower back, legs, and feet.…”