Effect of Leptin Gene Polymorphism on Reproductive Efficiency in Awassi Ewes

Abstract

L EPtin (LEP) is a protein release mostly from adipose tissue (the major source of LEP) (Houseknecht et al., 1998). It consists of 146 amino acids with molecular weight 16000 Dalton (Zhang et al., 1994). Chromosome four is containing the ovine LEP gene (Moravcikova et al., 2012). It's included two introns and three exons, the last two exons are involved in LEP protein synthesis (167 amino acids) which is undergo cleavage of 21 amino acid (signal peptide) (De La et al., 1996; Zhang et al., 1994). LEPtin receptors are categorized as class one cytokine receptors because of bilaterally symmetrical with a structure research Article Abstract | LEPtin (LEP) is a hormone that strongly associate with nutritional state, glucose homeostasis and reproduction. Study performed to identify the linkage between LEP polymorphism and reproductive efficiency such as Seasonality and litter size. Forty mature non-pregnant Awassi ewes were utilized between 1st July/2017 to 1st May/2018 in Salah Aldin province/Iraq. Twenty ewes were demonstrated estrus heat at August/2017which considered Seasonal group, and the others showed estrus signs at April/2018, which considered Non-Seasonal group.Genomic DNA was extracted from blood specimens and four primers were utilized to amplify exon II, intron II (fragment 1) and exon III (fragment 1) of LEP geneby polymerase chain reaction (PCR). Polymorphisms were revealed via sequencing and compared with the sequencing of the ovine LEP gene in NCBI. One single nucleotide polymorphism (SNP) A(99)R was detected in intron II and three SNPs G(425)R, T(541)K and G(587)R were in exon III. Two genotypes of each SNP were observed with higher significant differences (P<0.01) between frequencies 47.

Saanen Keçilerinde IGF-1/Cac8I, LEP/NmuCI ve PRLR/Hin1II Polimorfizmleri

“…Two SNPs in the exon 3 and intron 2 of LEP gene and their associations with milk traits were investigated by Kulig and Kmiec (2009) and it has been stated that LEP gene variations significantly affect milk, protein, and fat yield. Effects of LEP gene polymorphisms on reproductive efficiency in Awassi ewes was revealed by using DNA sequencing method and it was found significant associations between litter size and LEP polymorphisms (Younis et al 2019). 1).…”

PCR-SSCP and Sequencing Analysis For Studying Leptin Gene Polymorphism and Its Association with Reproductive Status of Egyptian Buffalo

“…Passos et al [50] described effect of leptin polymorphism on its expression on adipose tissues in beef cattle, when frequency of A allele was 0.91 and B allele was 0.09. Moreover, this polymorphism was associated with energy balance and fertility [50], milk yield [51,52] and somatic cell count in milk [53] and reproductive efficiency [54].…”

Candidate genes associated with reproductive traits in rabbits

“…Nevertheless, no studies addressed the association between LEP gene polymorphisms and prolificacy traits in rabbits. On the other hand, the LEP gene polymorphism was associated with litter size in pigs (Chen et al, 2004 ; Terman, 2005 ; Wang et al, 2011 ), goats (Wang et al, 2011 ; Alim et al, 2019 ), sheep (Mahmoud et al, 2014 ; Younis et al, 2019 ), as well as the association with reproductive performance in cows (Chebel and Santos, 2011 ; Trakovická et al, 2013 ).…”