Effect of Ligaria cuneifolia catechin- and quercetin-enriched fractions on hemorheology and plasma cholesterol

Abstract

We tested the in vivo and the in vitro effects of both Ligaria cuneifolia catechin-and quercetin-enriched fractions on erythrocyte shape and deformability, and on plasma cholesterol level. For in vivo studies, adult male Wistar rats were randomized in three experimental groups which received intraperitoneally, once a day, 3 days: Control: saline solution (C; n = 6); catechin from L. cuneifolia, 0.60 mg/100 g body weight (CLc; n = 6), or quercetin from L. cuneifolia, 2.3 mg/100 g body weight (QLc; n = 6). For in vitro studies, blood samples obtained from male Wistar rats were divided into three fractions, which were incubated with saline solution (C), catechin (CLc; n = 5) and quercetin (QLc; n = 5), in a concentration equivalent to 0.60 mg/100 g body weight, and 2.3 mg/100 g body weight, respectively. CLc significantly reduced the rigidity index due to a diminished mean concentration volume. QLc induced erythrocyte rigidization (less deformability), thus increasing blood viscosity. Neither of the two treatments produced any changes in plasmatic or biliary excretion of cholesterol. Opposite results were observed in rigidity index with CLc and QLc. In vitro studies showed an interaction of both CLc and QLc with the erythrocyte membrane, which induced changes in the erythrocyte shape from discocyte to stomatocyte. A. Dominighini et al. / Effect of Ligaria cuneifolia catechin-and quercetin-enriched fractions

Rapid Insulin-Mediated Increase in Microvascular Glycocalyx Accessibility in Skeletal Muscle May Contribute to Insulin-Mediated Glucose Disposal in Rats

“…Compared to the increases in capillary tube hematocrit of ∼250% found after bradykinin and SNP administration [10], the increases in hematocrit found with insulin were, however, relatively small, indicating that there still seems potential for capillary blood volume to increase further. Of note, MCV was based on previous studies [24], [25]; while effects of insulin or hyaluronidase on MCV have not been reported, possible affects of a change in MCV on capillary tube hematocrit in the present study cannot be ruled out.…”

“…where MCV is the mean corpuscular volume in rats (±70 um 3 ) [24], [25]. Using frame by frame playback RBC flux in a capillary was determined, at least 5 times per measurement, by counting the number of RBCs to pass through a reference point in 1 second.…”

Estudios de las propiedades medicinales de extracto total y distintas fracciones de Ligaria cuneifolia (R. et P.) Tiegh (Loranthaceae).

“…Estos cambios podrían deberse a la interacción de QLc o de catLc con la membrana eritrocitaria y a una disminución del volumen eritrocitario. Estos resultados sugieren que otro componente de Lc sería el responsable del descenso de Co plasmático observado (23).…”

Antibacterial activity of methanolic and aqueous extracts of Ligaria cuneifolia and Tripodhantus flagellaris.

“…In folk medicine, the infusion of these plants are used as hypotensive and to reduce excess cholesterol. Also, their anti-carcinogenic, anti-diabetic and anti-HIV activities have been reported (Dominighini et al, 2010;Fernandez et al, 1998). Tripodanthus fl agellaris is used mainly as cardioactive drug in Cuyo region (Argentina) (Del Vitto et al, 1997;Fusco et al, 2010).…”

“…There are some studies on the biological activities of L. cuneifolia and T. fl agellaris (Fusco et al, 2001;Fusco et al, 2004;Fusco et al, 2010;Ferrero et al, 2006;Dominighini et al, 2010). Few researchers have reported antibacterial activity of Loranthaceae species (Varela et al, 2001;Ukwueze et al, 2013).…”