Effect of Lithium on Bone Metabolism in Organ Culture*

Holak, H; Raisz, Lawrence G.

doi:10.1210/endo-104-4-908

Cited by 11 publications

(4 citation statements)

References 18 publications

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“…Lithium can affect CAMP-dependent events in various cell types (Dousa and Hechter, 1970;Anderson and Blackard, 1978;Gibson et al, 1979;Holak and Raisz, 1979). Recently lithium was also reported to abrogate CAMP-dependent events in human lymphocytes induced by cAMP activators but not by dibutyryl cAMP (Gelfand et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

Growth effect of lithium on mouse mammary epithelial cells in serum‐free collagen gel culture

Tomooka¹,

Imagawa²,

Nandi³

et al. 1983

Journal Cellular Physiology

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The effect of lithium on the growth of mammary epithelial cells from adult virgin and midpregnant BALB/c or BALB/cfC3H mice was tested in a serum-free collagen gel culture system. The serum-free medium consisted of a 1:1 mixture of Ham's F12 and Dulbecco's Modified Eagle's medium supplemented with insulin, transferrin, cholera toxin, epidermal growth factor (EGF), and bovine serum albumin fraction V (BSA V). A multifold increase in cell number occurred during 10-12 days of culture in this medium. In dose-response studies in which the concentration of each component of this serum-free medium was varied in turn, the addition of LiCL (10 mM) enhanced growth at most concentrations of each factor. However, LiCl could not enhance growth in the absence of insulin or BSA V, but could replace EGF. The optimal concentration of LiCl was 5-10 mM; higher concentrations (20-80 mM) were toxic. KCl (1-10 mM) when added to the serum-free medium slightly stimulated growth; the addition of NaCl to the medium had little effect on growth. LiCl did not enhance the growth of cells from spontaneous mammary tumors of BALB/cfC3H mice.

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Section: Discussionmentioning

confidence: 99%

Growth effect of lithium on mouse mammary epithelial cells in serum‐free collagen gel culture

Tomooka¹,

Imagawa²,

Nandi³

et al. 1983

Journal Cellular Physiology

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“…Holak and Raisz [40], studying the effects of lithium on bone metabolism, demonstrated that lithium does not inhibit the incorporation of labeled proline in collagen and noncollagen protein. Collagen types synthesized by murine bone marrow cells and the effect of lithium chloride on collagen biosynthesis have been studied in vitro experiments [41].…”

Section: Bonementioning

confidence: 99%

Collagenous Tissues upon Lithium Treatment: A Quantitative Ultrastructural Study

Tzaphlidou

2004

The Scientific World JOURNAL

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In this review, the influence of lithium treatment in mouse, rat, and rabbit skin, liver, bone, and aorta, as well as arachnoid and dura mater collagen fibrils, is examined using electron microscopy and image processing. Structural changes (fibril architecture and diameter) are detected at the ultrastructural level in specimens from all lithium-treated tissues. The overall collagen fibril architecture is disturbed as compared with specimens from normal species. The mean diameter values of treated collagen fibrils are significantly smaller than those from controls in all tissues examined. The banding patterns of fibrils are normal in all cases. Measurements by a computerized method of measuring axial periodicity of fibrils indicate no effect of lithium on this parameter. Computer analysis shows no differences in charged amino acid composition between lithium-treated and -untreated samples. Under the present experimental conditions, lithium can induce permanent structural collagen alterations.

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“…The effects of lithium on the cellular level have only begun to be extensively investigated within the last decade [17À19]. Studies on side effects concern the haemopietic tissues [20], kidneys [21À23], bone metabolism [24], and skin [25À30].…”

Section: Introductionmentioning

confidence: 99%

Side effects of lithium on rat cranial arachnoid and dura mater collagen: A quantitative ultrastructural study

Tzaphlidou

2003

J Trace Elements Exper Med

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Lithium is widely used in medicine as an antidepressive drug. Despite abundant literature on the subject, questions on the effects of lithium on collagen, which is one of the major components of tissues and organs, are far from being answered. We have examined the effects of lithium chloride (1.5 meq/kg of body weight) on rat cranial arachnoid and dura mater collagen fibrils by electron microscopy and image analysis. Animals were sacrificed 1 day, 2, 6, and 12 months after the end of a 30-consecutive days experimental period. In all cases investigated, severe structural abnormalities in collagen fibrils, at the ultrastructural level, were found. These abnormalities consist of decreased mean diameter and irregularity of shape of collagen fibrils (when viewed in cross sections) as well as marked disorganization in the packing of the fibrils. The observed alterations seem to be permanent.

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Effect of Lithium on Bone Metabolism in Organ Culture*

Cited by 11 publications

References 18 publications

Growth effect of lithium on mouse mammary epithelial cells in serum‐free collagen gel culture

Growth effect of lithium on mouse mammary epithelial cells in serum‐free collagen gel culture

Collagenous Tissues upon Lithium Treatment: A Quantitative Ultrastructural Study

Side effects of lithium on rat cranial arachnoid and dura mater collagen: A quantitative ultrastructural study

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