Effect of long-term nitrite administration on browning of white adipose tissue in type 2 diabetic rats: A stereological study

Varzandi, Tarlan; Abdollahifar, Mohammad‐Amin; Rohani, Seyed Ali Haeri; Piryaei, Abbas; Zadeh‐Vakili, Azita; Jeddi, Sajad; Ghasemi, Asghar

doi:10.1016/j.lfs.2018.06.012

Cited by 19 publications

(8 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our and other models of diabetes T2DM, insulin and glucose levels will rise, mimicking human T2DM ( 25 , 26 ). In this study, similar to other studies that have used the HFD-STZ model of T2DM ( 27 ), plasma insulin levels increased, indicating insulin resistance. Similar to this study, other studies have shown that induction of menopause and stimulation of GPER in OVX-diabetic animals has no effect on insulin secretion ( 15 ).…”

Section: Discussionsupporting

confidence: 89%

The G-Protein-Coupled Estrogen Receptor Agonist Prevents Cardiac Lipid Accumulation by Stimulating Cardiac Peroxisome Proliferator-Activated Receptor α: A Preclinical Study in Ovariectomized-Diabetic Rat Model

Jafarynezhad

Shahbazian

Farhadi

et al. 2022

Int J Endocrinol Metab

View full text Add to dashboard Cite

Background: Type 2 diabetes mellitus (T2DM) is associated with cardiometabolic changes, and menopause exacerbates these conditions, leading to a greater risk of cardiovascular diseases (CVDs). The G protein-coupled estrogen receptor (GPER), which mediates the rapid effects of estrogen, has beneficial cardiac effects in both T2DM and menopause, but its mechanism of action is not well understood. Objectives: This study aimed to determine whether G1 as a selective GPER-agonist has beneficial effects on cardiac lipid metabolism in ovariectomized rats with T2DM. Methods: Female Wistar rats were divided into 5 groups (n = 7 in each group): Sham-control (Sh-Ctl), T2DM, ovariectomized-T2DM (OVX-T2DM), OVX-T2DM-G1 (GPER-agonist), and OVX-T2DM-vehicle (OVX-T2DM-Veh). After stabilization of T2DM, G1 (200 μg/Kg) was administrated for 6 weeks. Then, the levels of free fatty acids (FFAs), CD36, peroxisome proliferator-activated receptor α (PPARα), and lipid accumulation in the cardiac tissue were determined. Results: Compared with the Sh-Ctl group, cardiac FFAs (P < 0.001), CD36 (P < 0.05), and lipid accumulation (P < 0.001) increased, and cardiac PPARα (P < 0.01) decreased in T2DM animals; ovariectomy intensified these changes. Also, cardiac FFAs, PPARα, and lipid accumulation (P < 0.05) significantly decreased in the OVX-T2DM-G1 group compared to the OVX-T2DM-Veh group. However, cardiac CD36 levels did not change. Conclusions: G1 as a selective GPER-agonist affects lipid metabolism in T2DM animals. It also plays a vital role in improving cardiac metabolism during postmenopausal diabetic conditions.

show abstract

Section: Discussionsupporting

confidence: 89%

The G-Protein-Coupled Estrogen Receptor Agonist Prevents Cardiac Lipid Accumulation by Stimulating Cardiac Peroxisome Proliferator-Activated Receptor α: A Preclinical Study in Ovariectomized-Diabetic Rat Model

Jafarynezhad

Shahbazian

Farhadi

et al. 2022

Int J Endocrinol Metab

View full text Add to dashboard Cite

show abstract

“…This in turn promotes inflammation and oxidative stress. Nevertheless, previous studies have demonstrated that dietary nitrate and nitrite administration was associated with increased nitrate and nitrite levels in adipose tissue in obesity and T2DM [12,40,41]. Our study underlines the importance of nitrate in MetS and showed that exogenous (i.e., dietary) nitrite/nitrate intake does not significantly affect the relationship between nitrate and MetS markers.…”

Section: Discussionsupporting

confidence: 51%

Plasma Nitrate Levels Are Related to Metabolic Syndrome and Are Not Altered by Treatment with DPP-4 Inhibitor Linagliptin: A Randomised, Placebo-Controlled Trial in Patients with Early Type 2 Diabetes Mellitus

et al. 2021

View full text Add to dashboard Cite

The depletion of nitrate and nitrite, stable nitric oxide (NO) end-products, promotes adipose tissue dysfunction and insulin resistance (IR). Dipeptidyl peptidase-4 (DPP-4) inhibitors have the potentially beneficial side effect of increasing NO availability. In this study, nitrate and nitrite levels and the effects of DPP-4 inhibitor linagliptin were investigated in relation to metabolic syndrome (MetS) markers. Treatment-naive patients with early type 2 diabetes mellitus (T2DM) (n = 40, median age 63 IQR (55–67) years, 63% male, mean HbA1c 45 ± 4.4 mmol/mol) were randomized (1:1) to linagliptin (5 mg/day) or placebo. MetS-related markers (body mass index (BMI), triglycerides, HOMA-IR, gamma-glutamyltransferase (GGT), C-reactive protein (CRP), and adiponectin), plasma levels of nitrate, nitrite, total free thiols (TFT) and vegetable intake were estimated at baseline and after 4 and 26 weeks of treatment. Plasma nitrate, but not nitrite, correlated positively with vegetable intake (r = 0.38, p = 0.018) and was inversely associated with HOMA-IR (r = −0.44, p = 0.006), BMI (r = −0.35, p = 0.028), GGT (r = −0.37, p = 0.019) and CRP (r = −0.34, p = 0.034). The relationship between nitrate and HOMA-IR remained significant after adjusting for BMI, CRP, vegetable intake and GGT. With stable vegetable intake, nitrate and nitrite, TFT, adipokines and CRP did not change after 26 weeks of linagliptin treatment. While plasma nitrate is inversely associated with MetS, linagliptin treatment does not significantly influence nitrate and nitrite concentrations, oxidative stress, adipose tissue function and systemic inflammation.

show abstract

“…In this study, sodium nitrate preconditioning for 60 days decreased blood glucose levels in diabetic rats, findings that also reported by previous studies. In this context, it has also been shown that nitrite decreased serum glucose concentration in diabetic rats by 27.6% in type 2 diabetic rats [43]. Moreover, infusion of sodium nitroprusside in type 2 diabetic patients resulted in higher glucose uptake independent of plasma insulin level [44].…”

Section: Discussionmentioning

confidence: 92%

Sodium nitrate preconditioning prevents progression of the neuropathic pain in streptozotocin-induced diabetes Wistar rats

Oghbaei

Mohaddes

Hamidian

et al. 2020

J Diabetes Metab Disord

View full text Add to dashboard Cite

Purpose The purpose of the study was to evaluate the possible protective effects of low dose sodium nitrate preconditioning on the peripheral neuropathy in streptozotocin (STZ)-induced diabetic model. Methods Male Wistar rats were randomly divided into five groups: control (no intervention), control treated sodium nitrate (100 mg/L in drinking water), diabetic (no intervention), diabetic treated NPH insulin (2-4 U), and diabetic treated sodium nitrate (100 mg/L in drinking water). Diabetes was induced by intraperitoneal injection of STZ (60 mg/kg). All interventions were done for 60 days immediately following diabetes confirmation. Thermal and mechanical algesia thresholds were measured by means of hot-plate test, von Frey test, and tail-withdrawal test before the diabetic induction and after diabetes confirmation. At the end of the experiment, serum NOx level and serum insulin level were assessed. Blood glucose concentration and body weight have recorded at the base and duration of the experiment. Results Both hypoalgesia, hyperalgesia along with allodynia developed in diabetic rats. Significant alterations including, decrease in tail withdrawal latency (30th day), decreased mechanical threshold (60th day), and an increase in hot plate latency (61st day) were displayed in diabetic rats compared to control rats. Nitrate and insulin preconditioning produced protective effects against diabetesinduced peripheral neuropathy. Data analysis also showed a significant increase in glucose level as well as a considerable reduction in serum insulin and body weight of diabetic rats, which restored by both insulin and nitrate preconditioning. Conclusion Sodium nitrate preconditioning produces a protective effect in diabetic neuropathy, which may be mediated by its antihyperglycemic effects and increased serum insulin level. KeywordsNitrate preconditioning . Diabetes . Peripheral nerve . Thermal algesia . Mechanical algesia Highlights • Diabetes decreased mechanical threshold (mechanical allodynia) • Diabetes caused the first reduction and then a secondary increase in tail withdrawal and hot plate latency • Sodium nitrate preconditioning decreased the blood glucose and increased the serum insulin level in diabetic rats. • Sodium nitrate preconditioning reduced allodynia and thermal algesia in diabetic rats * Rana Keyhanmanesh

show abstract

Effect of long-term nitrite administration on browning of white adipose tissue in type 2 diabetic rats: A stereological study

Cited by 19 publications

References 47 publications

The G-Protein-Coupled Estrogen Receptor Agonist Prevents Cardiac Lipid Accumulation by Stimulating Cardiac Peroxisome Proliferator-Activated Receptor α: A Preclinical Study in Ovariectomized-Diabetic Rat Model

The G-Protein-Coupled Estrogen Receptor Agonist Prevents Cardiac Lipid Accumulation by Stimulating Cardiac Peroxisome Proliferator-Activated Receptor α: A Preclinical Study in Ovariectomized-Diabetic Rat Model

Plasma Nitrate Levels Are Related to Metabolic Syndrome and Are Not Altered by Treatment with DPP-4 Inhibitor Linagliptin: A Randomised, Placebo-Controlled Trial in Patients with Early Type 2 Diabetes Mellitus

Sodium nitrate preconditioning prevents progression of the neuropathic pain in streptozotocin-induced diabetes Wistar rats

Contact Info

Product

Resources

About