Effect of low‐level laser therapy on intramembranous and endochondral autogenous bone grafts healing

Abstract

The aim of this study was to evaluate the healing process of intramembranous (IM) and endochondral (EC) bone grafts under low‐level laser therapy (LLLT). Male rabbits underwent onlay autogenous bone grafts (1 cm in diameter) retrieved from the calvaria and iliac crest and fixed on parietal bones, divided into four groups: Calvaria (C), Iliac (I), Calvaria + LLLT (C+L), and Iliac + LLLT (I+L). Animals from C+L and I+L Groups had their grafts exposed to LLLT (AlGaAs–808 nm, CW, 30 mW, 0.028 cm2 average laser beam area), 15 s irradiation time (16 J/cm2 per point–total of 64 J/cm2 per session). After 7, 14, 30, and 60 days, grafts were retrieved and resorption pattern analyzed by means of morphometry and TRAP‐positive osteoclasts detection. Differences in the resorption levels of iliac grafts were observed, presenting 40% in I group against 8% in I+L grafts at the 14th day of evaluation (P < 0.05). After 30 days, resorption was maintained at 41% in I group, whereas I+L presented 15% in the same period (P = 0.0591). No significant differences were noted in the rates of calvaria grafts resorption in all periods. A significant higher number of osteoclasts on the grafts' surface was observed in C+L Group at day 30, in comparison with C group. In I+L Group, prevalence of osteoclasts was marked at day 7 (P < 0.05) in comparison to I Group. In general, it was concluded that biomodulative effects of LLLT did not significantly affect healing and resorption processes of autogenous bone grafts from EC and IM origins. Microsc. Res. Tech. 75:1237–1244, 2012. © 2012 Wiley Periodicals, Inc.

Analysis of the effects of concentrated growth factor and low-level laser therapy on the bone healing

“…Kim et al prepared 8 mm diameter rabbit calvarial full-thickness bone defects to study the ability of Nd:YAG laser on the regeneration of bone defects, and found that the high-intensity laser could accelerate bone regeneration at 8 weeks, but could not completely heal [ 14 ]. Drawing on the experience of previously published papers, this study selected 8 mm as the critical bone defect [ 12 , 14 , 16 ]. Observation period of this study was 8 weeks, and the 8 mm diameter bone defect was in line with the critical bone defect.…”

Avaliação da cicatrização de defeitos ósseos criados cirurgicamente em calvárias de ratos e tratados com enxertos de origem bovina (Bio-Oss®) associados ao laser de baixa intensidade. Estudo histológico e histométrico

“…Embora o tecido ósseo apresente boa capacidade regenerativa, seu processo de cicatrização pode ser prejudicado em decorrência de um pobre suprimento sanguíneo, instabilidade mecânica, bem como a presença de tecidos com maior capacidade proliferativa (ABOELSAAD et al, 2009;GERBI, 2006). Diante disso, a utilização do laser de baixa intensidade (LB) como alternativa para acelerar a cicatrização em defeitos ósseos maiores tem sido estudada (BIGUETTI et al, 2012;FAVARO-PIPI et al, 2011;SILVA JUNIOR et al, 2002). (NISSAN et al, 2006).…”

“…Atualmente, modelos que envolvem análise da regeneração óssea usam, em sua metodologia, microscopia óptica (MARQUEZ MARTINEZ;RAMALHO, 2008;PINHEIRO et al, 2009a;PINHEIRO et al, 2009b), morfometria CAMILLI, 2006), imuno-histoquímica (BIGUETTI et al, 2012;MATSUMOTO et al, 2009), ou testes biomecânicos (CACCIAFESTA et al, 2001;JONES et al, 2007;KAZEM SHAKOURI et al, 2010;LUGER et al, 1998).…”