Effect of Media Composition on the Penicillin Production

El-Marsafy, M. K.; Abdel‐Akher, Mamdouh; El‐Saied, Hani M.

doi:10.1016/s0044-4057(77)80053-1

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…This might have induced the production of bioactive metabolites. Similar findings have also been reported in many studies, whereby media composition play a major role in the production of the antibiotics and/or are found to enhance the production of antibiotics (El-Marsafy et al 1977;Gesheva et al 2005;Antoniao et al 2012). However, if we compare the level of metabolites produced in these media, the level produced in ISP2 is nevertheless higher than YIM61# (Table 3).…”

Section: Discussionsupporting

confidence: 88%

Symbiotic bacteria associated with puffer fish Gastrophysus spadiceus and evaluation of their antimicrobial activities

et al. 2017

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The present study reports the diversity of culturable bacteria associated with the puffer fish . During the study, a total of 31 strains affiliated to the genera, , and were isolated from liver, intestines, and flesh of. These strains exhibited a diverse range of metabolites as indicated by the HPLC and TLC profiles of the chemical extracts of their fermentation products. Some of these crude extracts showed strong antimicrobial activities against pathogenic bacterial strains. In addition, few crude extracts exhibit insecticidal activity against .

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Section: Discussionsupporting

confidence: 88%

Symbiotic bacteria associated with puffer fish Gastrophysus spadiceus and evaluation of their antimicrobial activities

et al. 2017

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“…However, all the strains are unable to utilize the higher precursor concentration and may affect growth and PenV production. Conventional penicillin production in a defined medium containing corn steep liquor, glucose, lactose, minerals, oil, and specific precursors enhances the production of the P. chrysogenum Q176 strain [ 75 , 76 , 77 ]. Inoculum size, temperature, incubation period, and initial pH majorly affects the penicillin titer [ 78 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Molecular Characterization of Indigenous Penicillium chrysogenum/rubens Strain Portfolio for Penicillin V Production

2023

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Beta (β)-lactam antibiotic is an industrially important molecule produced by Penicillium chrysogenum/rubens. Penicillin is a building block for 6-aminopenicillanic acid (6-APA), an important active pharmaceutical intermediate (API) used for semi-synthetic antibiotics biosynthesis. In this investigation, we isolated and identified Penicillium chrysogenum, P. rubens, P. brocae, P. citrinum, Aspergillus fumigatus, A. sydowii, Talaromyces tratensis, Scopulariopsis brevicaulis, P. oxalicum, and P. dipodomyicola using the internal transcribed spacer (ITS) region and the β-tubulin (BenA) gene for precise species identification from Indian origin. Furthermore, the BenA gene distinguished between complex species of P. chrysogenum and P. rubens to a certain extent which partially failed by the ITS region. In addition, these species were distinguished by metabolic markers profiled by liquid chromatography–high resolution mass spectrometry (LC-HRMS). Secalonic acid, Meleagrin, and Roquefortine C were absent in P. rubens. The crude extract evaluated for PenV production by antibacterial activities by well diffusion method against Staphylococcus aureus NCIM-2079. A high-performance liquid chromatography (HPLC) method was developed for simultaneous detection of 6-APA, phenoxymethyl penicillin (PenV), and phenoxyacetic acid (POA). The pivotal objective was the development of an indigenous strain portfolio for PenV production. Here, a library of 80 strains of P. chrysogenum/rubens was screened for PenV production. Results showed 28 strains capable of producing PenV in a range from 10 to 120 mg/L when 80 strains were screened for its production. In addition, fermentation parameters, precursor concentration, incubation period, inoculum size, pH, and temperature were monitored for the improved PenV production using promising P. rubens strain BIONCL P45. In conclusion, P. chrysogenum/rubens strains can be explored for the industrial-scale PenV production.

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Omics Approaches Applied to Penicillium chrysogenum and Penicillin Production: Revealing the Secrets of Improved Productivity

Garcı́a-Estrada

Martı́n

Cueto

et al. 2020

Genes

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Penicillin biosynthesis by Penicillium chrysogenum is one of the best-characterized biological processes from the genetic, molecular, biochemical, and subcellular points of view. Several omics studies have been carried out in this filamentous fungus during the last decade, which have contributed to gathering a deep knowledge about the molecular mechanisms underlying improved productivity in industrial strains. The information provided by these studies is extremely useful for enhancing the production of penicillin or other bioactive secondary metabolites by means of Biotechnology or Synthetic Biology.

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Effect of Media Composition on the Penicillin Production

Cited by 3 publications

References 8 publications

Symbiotic bacteria associated with puffer fish Gastrophysus spadiceus and evaluation of their antimicrobial activities

Symbiotic bacteria associated with puffer fish Gastrophysus spadiceus and evaluation of their antimicrobial activities

Isolation and Molecular Characterization of Indigenous Penicillium chrysogenum/rubens Strain Portfolio for Penicillin V Production

Omics Approaches Applied to Penicillium chrysogenum and Penicillin Production: Revealing the Secrets of Improved Productivity

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