Effect of melatonin on the severity of L-arginine-induced experimental acute pancreatitis in rats

Szabolcs, Annamária; Hegyi, P.; Pápai, Gábor; Varga, I; Kaszaki, József; Letoha, Tamás; Sári, Réka; Lonovics, János; Rj, Reiter

doi:10.1055/s-2004-827042

Cited by 8 publications

(11 citation statements)

References 36 publications

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“…This model is ideal due to its non-invasive and non-invasive nature and the fact that it causes dose-dependent acinar cell necrosis, allowing studies on the pathogenesis of acute pancreatitis. Szaboles et al 12 have also used L-arginine to develop pancreatitis from which they have earned good results.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic effects of ellagic acid on L-arginin ınduced acute pancreatitis

et al. 2016

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PURPOSE:To investigate the therapeutic effects of ellagic acid on L-arginin ınduced acute pancreatitis in rats. METHODS:Thirty-two were split into four groups. Group 1 (control) rats were performed only laparotomy, no drugs were administered. Group 2 (control+EA) rats were administered 85mg/kg EA orally. Rats were sacrificed by cardiac puncture 24 hours after the administration. Group3 (AP) 24 hours after intraperitoneal L-arginine administration, rats were sacrificed by cardiac puncture.Group 4 (EA)-(AP): 85mg/kg EA was administered orally after the L-arginine administration. 24 hours later, rats were sacrificed by cardiac puncture. Serum TNF-α, IL-1β, IL-6, total oxidative status (TOS), total antioxidant capacity (TAC), amylase levels were determined in all groups. RESULTS:Group 3 (AP) rats showed significantly raised TOS level as compared to Group1 (control) rats (p<0.001). Following the EA therapy, a decrease in TOS was observed in Group 4 (AP+EA). TAC levels were significantly raised in the Group 4 (AP+EA) compared to the Group 3 (AP) (p=0.003). Group 3 (AP) showed significantly increased TNF-α, IL-1β and IL-6 serum levels as compared to Group 4 (AP+EA). Histopathological changes were supported our result. CONCLUSION:The healing effects of ellagic acid on inflammatory and oxidative stress were confirmed by histopathological and biochemical evaluations of the pancreatic tissue.

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Section: Introductionmentioning

confidence: 99%

Therapeutic effects of ellagic acid on L-arginin ınduced acute pancreatitis

et al. 2016

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“…Melatonin exerts its beneficial effects through several mechanisms, including scavenging free radicals, activating antioxidative enzymes, inhibiting neutrophil infiltration, promoting neutrophil apoptosis, modulating cytokine production and altering the generation of pancreatic prostaglandins [10,11,18,22,30,46,50]. In addition, the melatonin precursor, L-tryptophan, increases the level of melatonin in the blood and attenuates AP in rats [22,26,50].…”

Section: Discussionmentioning

confidence: 99%

Pinealectomy aggravates acute pancreatitis in the rat

Jaworek

Żwirska-Korczala

Szklarczyk

et al. 2010

Pharmacological Reports

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Abstract:Melatonin, a pineal indoleamine, protects the pancreas against acute damage; however, the involvement of the pineal gland in the pancreatoprotective action of melatonin is unknown. The primary aim of this study was to determine the effects of pinealectomy on the course of acute caerulein-induced pancreatitis (AP) in rats. AP was induced by a subcutaneous infusion of caerulein (25 µg/kg) into pinealectomized or sham-operated animals. Melatonin (5 or 25 mg/kg) was given via intraperitoneal (ip) injection 30 min prior to the induction of AP. The pancreatic content of the lipid peroxidation products malondialdehyde and 4-hydroxynonenal (MDA + 4HNE) and the activity of an antioxidative enzyme, glutathione peroxidase (GSH-Px), were measured in each group of rats. Melatonin blood levels were measured by radioimmunoassay (RIA). In the sham-operated rats, AP was confirmed with histological examination and manifested as pancreatic edema and an increase in the blood lipase level (by 1,500%). In addition, the pancreatic content of MDA + 4HNE was increased by 200%, and pancreatic glutathione peroxydase (GSH-Px) activity was reduced by 40%. Pinealectomy significantly aggravated the histological manifestations of AP, reduced the GSH-Px activity and markedly augmented the levels of MDA + 4HNE in the pancreas of rats with or without AP as compared to sham-operated animals. Melatonin was undetectable in the blood of the pinealectomized rats with or without AP. Treatment with melatonin (25 mg/kg, ip) prevented the development of AP in the sham-operated rats and significantly reduced pancreatic inflammation in the animals previously subjected to pinealectomy. In conclusion, pineal melatonin contributes to the pancreatic protection through the activation of the antioxidative defense mechanism in pancreatic tissue as well as its direct antioxidant effects.

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“…The change in levels of catalase and SOD remains controversial. Czako and Takacs 25 reported the fall in these enzyme levels at 24 h. However, Szabolcs 24 reported the raised levels of these enzymes. In consistent with Robbins, 2 in the present study significant increase in SOD and catalase level was observed.…”

Section: Assessment Of Interleukins and C-reactive Proteinmentioning

confidence: 95%

“…In consistent with previous reports alpha-pinene has significantly reduced the raised level of acinar cell necrosis, serum amylase and lipase. 5,18,19 One of the important diagnostic markers for acute pancreatitis are serum amylase and lipase, which rise within four to eight hours of initial attack and will be peak at 24 hours. Treatment with alpha-pinene decreased the serum amylase and lipase levels, indicating protective effect of alpha-pinene at early stage of the disease progression.…”

Section: Assessment Of Interleukins and C-reactive Proteinmentioning

confidence: 99%

“…One of the lipid peroxidation marker, which is elevated in L-arginine treated rats. Lipid peroxidation is a process mediated by free radicals, which results in impairment of the membrane functional and structural integrity 7,24,25 resulting in oxidative deterioration of polyunsaturated fatty acids of cell membrane. It could be attributed to the accumulation of free radicals proposed to be generated by L-arginine.…”

Section: Assessment Of Interleukins and C-reactive Proteinmentioning

confidence: 99%

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Current Scenario of Patent Act: Compulsory Licensing

Chander¹,

Choudhary²,

Kumar³

2013

IJPER

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Objective: Screening of alpha-pinene on L-arginine induced acute pancreatitis model. Material and Methods: In this model the acute pancreatitis was determined at 24h by determination of pancreatic wet weight/body weight ratio, nitrate/nitrite levels, lipid peroxidation (thiobarbituric acid reactive substances (TBARS)], proinflammatory cytokines [tumor necrosis factor (TNF)-α, C-reactive proteins (CRP) and interleukin (IL)], pancreatic myeloperoxidase (MPO) activity and serum levels of amylase and lipase. Results: Administration of L-arginine had induced pancreatitis and this was characterized by pulmonary complication including altered pancreatic wet weight/body weight ratio, nitrate/nitrite levels, proinflammatory cytokines, TBARS, MPO and serum levels of amylase and lipase. Treatment with alpha-pinene (100 and 200mg/kg) dose significantly attenuated the L-arginine-induced pancreatitis and related complication. Conclusion: The histological findings proved the amelioration of pancreatic injury by alphapinene and biochemical parameters proved anti-inflammatory and antioxidant property of alpha-pinene.

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Effect of melatonin on the severity of L-arginine-induced experimental acute pancreatitis in rats

Cited by 8 publications

References 36 publications

Therapeutic effects of ellagic acid on L-arginin ınduced acute pancreatitis

Therapeutic effects of ellagic acid on L-arginin ınduced acute pancreatitis

Pinealectomy aggravates acute pancreatitis in the rat

Current Scenario of Patent Act: Compulsory Licensing

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