Effect of methyl jasmonate and silver nanoparticles on production of secondary metabolites by &lt;i&gt;Calendula officinalis&lt;/i&gt; L (Asteraceae)

Ghanati, Faezeh; Bakhtiarian, Somyeh

doi:10.4314/tjpr.v13i11.2

Cited by 73 publications

(31 citation statements)

References 18 publications

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“…31 The amount of saponin content in Calendula officinalis increased compared to the control roots after treatment with 0.4 mM nanosilver. 32 Due to the importance of the pharmaceutical and industrial applications of RA, its production has been studied in various plant species (Orthosiphon aristatus, Salvia miltiorrhiza, and Melissa officinalis) influenced by biological and non-biological elicitors. [33][34][35] In Agastache foeniculum hairy root cultures, a fourfold increase in RA content was observed.…”

Section: Introductionmentioning

confidence: 99%

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

et al. 2019

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BACKGROUND Dracocephalum kotschyi Boiss. is a valuable source of rosmarinic acid (RA) and methoxylated hydroxyflavones (such as xanthomicrol and cirsimaritin) with antioxidative and antiplatelet effects and with antiproliferative potential against various cancer cells. The extensive application of nanotechnology in hairy root cultures is a new sustainable production platform for producing these active constituents. In the present study, hairy roots derived from 4‐week‐old leaves and Agrobacterium rhizogenes strain ATCC15834 were used to investigate the impact of various concentrations of iron oxide nanoparticles (Fe NPs) in two elicitation time exposures (24 and 48 h) on growth, antioxidant enzyme activity, total phenolic and flavonoid content (TPC and TFC), and some polyphenols. Gene expression levels of phenylalanine ammonia‐lyase (pal) and rosmarinic acid synthase (ras) were also analyzed. RESULTS Iron nanoparticles enhanced biomass accumulation in hairy roots. The treatment time and Fe NP dosage largely improved the activity of antioxidant enzymes, TPC and TFC. The highest RA (1194 μg g−1 FW) content (9.7‐fold), compared to controls, was detected with 24 h of exposure to 75 mg L−1 Fe NP, which was consistent with the expression of pal and ras genes under the influence of elicitation. The xanthomicrol, cirsimaritin, and isokaempferide content was increased 11.87, 3.85, and 2.27‐fold, respectively. CONCLUSION Stimulation of D. kotschyi hairy roots by Fe NPs led to a significant increase in the induction and production of important pharmaceutical compounds such as rosmarinic acid and xanthomicrol. © 2019 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

et al. 2019

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“…Although there is concern about their side effects on the environment, these can be minimized by limiting the concentration of nanoparticle solutions. The application of silver nanoparticles in Calendula officinalis resulted in a decrease in the anthocyanin and flavonoid contents and an increase in carotenoids and saponins [9]. The easy penetration of ultra-fine nanoparticles into the plant cell wall and vacuoles disturbs metabolic activity.…”

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confidence: 99%

Influence of nano‐zinc oxide on tropane alkaloid production, h6h gene transcription and antioxidant enzyme activity in Hyoscyamus reticulatus L. hairy roots

Asl

Hosseini

Sharafi

et al. 2018

Engineering in Life Sciences

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The use of nanotechnology and biotechnology to improve the production of plant bioactive compounds is growing. Hyoscyamus reticulatus L. is a major source of tropane alkaloids with a wide therapeutic use, including treatment of Parkinson's disease and to calm schizoid patients. In the present study, hairy roots were obtained from two‐week‐old cotyledon explants of H. reticulatus L. using the A7 strain of Agrobacterium rhizogenes. The effects of different concentrations of the signaling molecule nano‐zinc oxide (ZnO) (0, 50, 100 and 200 mg/L), with three exposure times (24, 48 and 72 h), on the growth rate, antioxidant enzyme activity, total phenol contents (TPC), tropane alkaloid contents and hyoscyamine‐6‐beta‐hydroxylase (h6h) gene expression levels were investigated. Growth curve analysis revealed a decrease in fresh and dry weight of ZnO‐treated hairy roots compared to the control. ANOVA results showed that the antioxidant activity of the enzymes catalase, guaiacol peroxidase and ascorbate peroxidase was significantly higher in the ZnO‐treated hairy roots than in the control, as was the TPC. The highest levels of hyoscyamine (37%) and scopolamine (37.63%) were obtained in hairy roots treated with 100 mg/L of ZnO after 48 and 72 h, respectively. Semi‐quantitative RT‐PCR analysis revealed the highest h6h gene expression was in hairy roots treated with 100 mg/L of ZnO after 24 h. It can be concluded that ZnO is as an effective elicitor of tropane alkaloids such as hyoscyamine and scopolamine due to its enhancing effect on expression levels of the biosynthetic h6h gene.

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“…For instance, the artemisinin content was increased in a hairy root culture of Artemisia annua upon treatment with core–shell silver nanoparticles . In Calendula officinalis , the anthocyanin and flavonoid contents were decreased, whereas carotenoid and saponin contents were increased in response to silver nanoparticles, as revealed by classical colorimetric assays . In addition, multiwalled carbon nanotubes stimulated the biosynthesis of secondary metabolites and the antioxidant capacity in the medicinal plant Satureja khuzestanica grown in vitro .…”

Section: Future Perspectivesmentioning

confidence: 98%

“…[95] In Calendula officinalis, the anthocyanin and flavonoid contents were decreased, whereas carotenoid and saponin contents were increased in response to silver nanoparticles, as revealed by classical colorimetric assays. [96] In addition, multiwalled carbon nanotubes stimulated the biosynthesis of secondary metabolites and the antioxidant capacity in the medicinal plant Satureja khuzestanica grown in vitro. [97] In the case of H. perforatum, one study has reported that zinc and iron nano-oxides stimulate hypericin and hyperforin production in cell suspension cultures.…”

Section: Future Perspectives Potential Application Of Nanotechnologymentioning

confidence: 99%

Elicitation as a tool to improve the profiles of high-value secondary metabolites and pharmacological properties of Hypericum perforatum

Shakya

Marslin

Karthik

et al. 2019

Journal of Pharmacy and Pharmacology

126

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Objectives In this review, we aim at updating the available information on the improvement of the Hypericum perforatum L. (Hypericaceae) phytochemical profile and pharmacological properties via elicitation. Key findings Hypericum perforatum seedlings, shoots, roots, calli and cell suspension cultures were treated with diverse elicitors to induce the formation of secondary metabolites. The extracts of the elicitor-treated plant material containing naphthodianthrones, phloroglucinols, xanthones, flavonoids and other new compounds were quantitatively analysed and tested for their bioactivities. While hypericins were mainly produced in H. perforatum cultures containing dark nodules, namely shoots and seedlings, other classes of compounds such as xanthones, phloroglucinols and flavonoids were formed in all types of cultures. The extracts obtained from elicitor-treated samples generally possessed better bioactivities compared to the extract of control biomass. Summary Although elicitation is an excellent tool for the production of valuable secondary metabolites in H. perforatum cell and tissue cultures, its exploitation is still in its infancy mainly due to the lack of reproducibility and difficulties in scaling up biomass production.

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Effect of methyl jasmonate and silver nanoparticles on production of secondary metabolites by <i>Calendula officinalis</i> L (Asteraceae)

Abstract: Purpose: To investigate the effects of two elicitors, methyl jasmonat (MeJA) and silver nanoparticles (SNPs), on the production of secondary metabolite by Calendula officinalis L (marigold

Cited by 73 publications

References 18 publications

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

Influence of nano‐zinc oxide on tropane alkaloid production, h6h gene transcription and antioxidant enzyme activity in Hyoscyamus reticulatus L. hairy roots

Elicitation as a tool to improve the profiles of high-value secondary metabolites and pharmacological properties of Hypericum perforatum

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