Effect of methylprednisolone on perivascular pulmonary edema, inflammatory infiltrate, VEGF and TGF-beta immunoexpression in the remaining lungs of rats after left pneumonectomy

Guimarães-Fernandes, Flávio; Samano, Marcos Naoyuki; Carvalho, Celso Ricardo Fernandes; Pazetti, Rogério; Moreira, L.F.P.; Pêgo‐Fernandes, Paulo Manuel; Jatene, Fábio Biscegli

doi:10.1590/s0100-879x2011007500061

Cited by 1 publication

(1 citation statement)

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“…Attempts to enhance innate post-PNX responses using individual growth promoters in rodent models led to mixed cellular and structural effects (14,24,25,40,57) and few studies assessed the functional outcome on gas exchange. In our earlier canine studies (4,6,34,51,54), oral all-trans retinoic acid significantly enhanced active alveolar re-growth after right PNX (34,51) 54) and abrogated oxidative stress damage ( 6), but exerted only minor effects on extravascular alveolar tissue compartments.…”

Section: Interventions To Augment Compensatory Lung Growthmentioning

confidence: 99%

Inhalational delivery of induced pluripotent stem cell secretome improves postpneumonectomy lung structure and function

Dane

Cao

Zhang

et al. 2020

Journal of Applied Physiology

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Cell-free secretory products (secretome) of human induced pluripotent stem cells (iPSCs) have been shown to attenuate tissue injury and facilitate repair and recovery. To examine whether iPSC secretome facilitates mechanically-induced compensatory responses following unilateral pneumonectomy (PNX), litter-matched young adult female hounds underwent right PNX (removing 55-58% of lung units) followed by inhalational delivery of either the nebulized conditioned media containing iPSCs secretome (iPSC CM) or control cell-free media (CFM); inhalation was repeated every 5 days for 10 treatments. Lung function was measured under anesthesia pre-PNX and 10 d after the last treatment (8 weeks post-PNX); detailed quantitative analysis of lung ultrastructure was performed postmortem. Pre-PNX lung function was similar between groups. Compared to CFM control, treatment with iPSC CM attenuated the post-PNX decline in DLCO and DMCO, accompanied by a 24% larger postmortem lobar volume and distal air space enlargement. Alveolar double-capillary profiles were 39% more prevalent consistent with enhanced intussusceptive angiogenesis. Frequency distribution of the harmonic mean thickness of alveolar blood-gas barrier shifted towards the lowest values while alveolar septal tissue volume and arithmetic septal thickness were similar, indicating septal remodeling and reduced diffusive resistance of the blood-gas barrier. Thus, repetitive inhalational delivery of iPSC secretome enhanced post-PNX alveolar angiogenesis and septal remodeling that are associated with improved gas exchange compensation. Results highlight the plasticity of the remaining lung units following major loss of lung mass that are responsive to broad-based modulation provided by the iPSC secretome.

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