Effect of Mobile Phase Variation and Purification on Chromatogram View by Using Fruits and Rose Extracts and HPLC Method

Abstract

In this study extracts obtained from fruits of bluebery and cranberry and rose leaves were chromatografied by using mobile phase variation and DAD detector. The chromatograms were evaluated under different mobile phase systems such as isocratic and gradient elutions following purification procedure. The best separation conditions of pigments of anthocyanin in extracts and efficiency of purification procedure were searched by using mobile phases in the elution of isocratic and gradient modes. The chromatogram views have shown existence of anthocyanin constituents in the all fruits and rose extracts involving separation and qualitation. The effect of purification for separation and removal of pigments has been investigated.

Advances in the Chemistry, Analysis and Adulteration of Anthocyanin Rich-Berries and Fruits: 2000–2022

“…The detection of anthocyanins is often performed by Diode Array Detector (DAD) in mass-spectrometry (both MS and MS/MS) which is often coupled with an electrospray ionization source (ESI) [ 76 ]. These methodologies have shown satisfactory results for identifying and quantifying anthocyanins [ 15 , 40 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , <...…”

“…In 2021, Li et al reported a study to assess the efficiency of extraction methods from conventional (solvent and ultrasonic extraction) to enzymatic extraction for anthocyanins and their antioxidant properties following each extraction method [ 134 ]. Several reports [ 83 , 97 , 102 , 104 , 106 , 108 , 109 , 110 , 111 , 112 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 ] observed that the quantity of anthocyanins in blueberries varies based on the place, collecting/harvesting season, extraction solvent, extraction method, and method of analysis.…”