Effect of molybdenum and tungsten on synthesis and composition of formate dehydrogenase in Methanobacterium formicicum

Abstract

The influence of iodium molybdate amg sodium tate on fomate dehydave ity was stud in H2-C02-grw cues of Mathwobactenum fornicicur. Depletion

Properties of the tungsten‐substituted molybdenum formylmethanofuran dehydrogenase from Methanobacterium wolfei

“…This has been shown, for example, for nitrogenas¢ from A:atabacter vinelandU [12], nitrate reductas¢ from plants [13], hepatic sulphit= oxidase [14], and formate dehydrog=nase from Methanabaeterium fomzt¢icum [15]. Rest activity of the enzymes can be attributed to small amounts of' molybdenum still pr¢s=nt.…”

Corynebacterium glutamicum harbours a molybdenum cofactor-dependent formate dehydrogenase which alleviates growth inhibition in the presence of formate

“…in Methylobacterium extorquens (Laukel et al, 2003) or in R. eutropha (Cramm, 2009). In various studies it was shown that the interchange of molybdenum and tungsten led to inactive enzymes which can be exploited to identify whether the enzyme of interest is molybdenumor tungsten-dependent (May et al, 1988;McMaster & Enemark, 1998). However, for the N-formylmethanofuran dehydrogenases from Methanobacterium thermoautotrophicum and Methanobacterium wolfeii the interchange of molybdenum and tungsten yields enzymes with comparable activities (Johnson et al, 1996).…”

Effect of tungsten and molybdenum on growth of a syntrophic coculture of Syntrophobacter fumaroxidans and Methanospirillum hungatei

“…The stimulating eVect of both the elements was found to be related to either the FDH levels in methanogens growing on formate, such as M. formicicum and Methanococcus vannielii (Jones and Stadtman 1981;May et al 1988), or to the FMDH level in methanogens growing on H 2 /CO 2 such as Methanothermobacter wolfei and Methanothermobacter marburgensis (Bertram et al 1994;Hochheimer et al 1998). Growth of M. formicicum and M. vannielii was not aVected by Mo or W when H 2 /CO 2 was the substrate instead of formate, as FDH is not required for autotrophic growth (Jones and Stadtman 1981;May et al 1988). Similar to these two methanogens, M. hungatei grows on both H 2 /CO 2 and formate.…”

“…Since the chemical and catalytic properties of these two metals are very similar, some enzymes contain either Mo or W, and some microorganisms even contain analogues of enzymes containing either W or Mo, depending on the growth conditions (Bertram et al 1994;Hochheimer et al 1998;Stewart et al 2000;Brondino et al 2004). However, most microorganisms just possess one type of enzyme, in which the W and Mo can not eVectively replace each other (Jones and Stadtman 1977;Van Bruggen et al 1986;Zindel et al 1988;Afshar et al 1998), and in some cases, they even have antagonistic eVects (Ljungdahl and Andreesen 1978;Zellner and Winter 1987;May et al 1988).…”