Effect of Morphogenetic Protein BMP-2 on X-Ray Density of Bone Defect in the Experiment

Yarygin, N.V.; Parshikov, M. V.; Prosvirin, Alexander E.; Гурьев, В. В.; Говоров, М.В.; Bosykh, V G; Акатов, В. С.; Чеканов, А. В.

doi:10.1007/s10517-020-04755-3

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…The addition of 60 ng/ml BMP-2 exhibited enhanced osteoblast differentiation by regulating the expression of phospho-Smad1/5/8( 23 ). In rat animal models, BMP-2 in a concentration of 50 ng/ml increased the radiographic density of bone defects ( 25 ). However, preconditioning of mesenchymal stem cells with lower concentrations of 10 and 20 ng/ml of BMP-2 enhanced osteogenic differentiation ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression

2020

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Bone morphogenetic protein 2 (BMP-2) is a growth factor that is used to induce osteogenic differentiation in stem cells. The present study assessed the effects of BMP-2 on stem cell spheroid morphology, viability and osteogenic differentiation. Stem cell spheres were constructed and treated with BMP-2 at predetermined concentrations (0-100 ng/ml) using concave microwells. Cell viability was qualitatively and quantitatively analyzed via microscopy and a water-soluble tetrazolium salt assay kit, respectively. Alkaline phosphatase activity was assessed and an anthraquinone dye for calcium deposit evaluation was performed to determine osteogenic differentiation. The expressions of (runt-related transcription factor 2) and collagen 1 were also determined via quantitative PCR. Spherical shapes were formed using concave microwells on day 1, which were maintained up to day 21. On day 1, the relative cell viability of 0, 10 and 100 ng/ml BMP-2 treated cells was 100.0±1.9, 97.3±4.4 and 101.3±2.6%, respectively. Significantly higher values for alkaline phosphatase activity were determined in the 100 ng/ml treated group when compared with the control group. Additionally, Runx2 mRNA levels were significantly higher in the 100 ng/ml BMP-2 group compared with the control group, as determined via quantitative PCR. The results of the present study indicated that BMP-2 enhanced the differentiation of stem cell spheres, which was demonstrated by increased alkaline phosphatase activity and Runx2 expression.

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Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression

2020

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“…The osteoinductive growth factor bone morphogenetic protein-2 (BMP-2) has been broadly used as an effective alternative or adjuvant to autograft tissue or biomaterial scaffolds in clinical application [11,12]. Many animals and clinical studies have demonstrated that BMP-2 effectively induces bone formation [13][14][15]. Hence, the use of BMP-2 in combination with BBCP is likely to be even more effective than BMP-2 alone.…”

Section: Introductionmentioning

confidence: 99%

Collagen particles with collagen-binding bone morphogenetic protein-2 promote vertebral laminar regeneration in infant rabbits

et al. 2020

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The vertebral laminar defects caused by severe spina bifida occulta, spinal fracture, or bone tuberculosis require surgical treatment. The reconstruction of vertebral laminar defects remains challenging, especially in children. In this study, we created an animal model of vertebral laminar defects in newly weaned rabbits to evaluate the therapeutic effect of bovine bone collagen particle (BBCP) that combined with bone morphogenetic protein-2 with collagen binding domain (CBD-BMP-2). The tissues at the injury site which were harvested after 12 weeks indicated that newly formed bone was observed in both BBCP and BBCP/CBD-BMP-2 groups, whereas the injury site of the control group was mostly filled by fibrous tissue. The BBCP/CBD-BMP-2 group recovered better than the BBCP group. These findings indicate that a combination of BBCP with CBD-BMP-2 may be a good strategy for vertebral laminar defects in children.

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“…There was no significant difference between the groups, but the drug-loaded scaffold groups showed an increased cell proliferation rate on day 7. Previous studies reported that BMP-2 and OPG were mainly responsible for cell differentiation, with little effect on cell proliferation (Yarygin et al, 2020;Wang et al, 2021;Zhang et al, 2021). In addition, the images of Calcein-AM/PI staining indicated that the OP-BMSCs had good cell viability in all groups on day 3 (Figure 2D).…”

Section: The Biocompatibility Of the 3d-printed Composite Scaffoldsmentioning

confidence: 67%

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Effect of Morphogenetic Protein BMP-2 on X-Ray Density of Bone Defect in the Experiment

Cited by 5 publications

References 13 publications

Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression

Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression

Collagen particles with collagen-binding bone morphogenetic protein-2 promote vertebral laminar regeneration in infant rabbits

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Effect of Morphogenetic Protein BMP-2 on X-Ray Density of Bone Defect in the Experiment

Cited by 5 publications

References 13 publications

Bone morphogenetic protein&nbsp;2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression

Bone morphogenetic protein&nbsp;2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression

Collagen particles with collagen-binding bone morphogenetic protein-2 promote vertebral laminar regeneration in infant rabbits

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Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression

Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression