Effect of multiplication-stimulating activity (MSA) on the cyclic AMP level and proteoglycan synthesis in cultured chondrocytes

Tsuji, Masakatsu; Kato, Yukio; Nomura, Yoshio; Kinoshita, Masahiko; Kumahara, Yuichi; Suzuki, Fujio

doi:10.1530/acta.0.1040117

Cited by 5 publications

References 9 publications

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The Somatomedin Hypothesis of Growth Hormone Action

Hintz

1999

Comprehensive Physiology

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The sections in this article are: Early Studies on the Mechanism of Growth Hormone Action The Sulfation Factor Hypothesis Characterization of Somatomedin Biological Activity in Serum Stimulation of Differentiated Cell Functions Increases in DNA , RNA , and Protein Synthesis Synthesis and Control Mechanisms of Action Assays and Serum Levels of Somatomedin Purification of Somatomedin from Serum Biochemical Characterization The Isolation and Sequencing of Nonsuppressible Insulin‐Like Activity‐S Peptides and the Insulin‐Like Growth Factor Terminology Relationship to Nonsuppressible Insulin‐Like Activity‐S and Multiplicating Stimulating Activity Characterization of the Specific Somatomedin Peptides Development of Specific Assays Pathophysiology of Serum Levels Somatomedin Receptor Sites Somatomedin Binding Proteins Metabolism and Feedback on Growth Hormone Secretion Lessons from the RNA and DNA Structures of the Insulin‐like Growth Factors Paracrine Actions The Dual Effector Hypothesis of Growth Hormone Action Evidence that the Somatomedins are the Major Effectors of the Growth Actions of Growth Hormone Indirect Evidence Direct Evidence Epilogue

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The Somatomedin Hypothesis of Growth Hormone Action

Hintz

1999

Comprehensive Physiology

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Differential modulation of growth and phenotypic expression of chondrocytes in sparse and confluent cultures by growth factors in cartilage

Hiraki

Inoue

Asada

et al. 1990

Journal of Bone and Mineral Research

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The growth-promoting actions of cartilage extracts (CE) on rabbit cultured chondrocytes were studied to assess the role of local acting growth factors in the generation and expansion of highly differentiated cells. In the present study, DNA synthesis and proteoglycan synthesis in the cultured chondrocytes were monitored by flow cytofluorometry and double-isotope autoradiography by using [3H]thymidine and [35S]sulfate. We report here that actions of the same set of growth factors extracted from cartilage evokes differential cellular responses depending upon cell density. Growth factors in the optimal dose of CE (2 micrograms/ml) or epidermal growth factor (EGF, 40 ng/ml) did not reveal such a cell density-dependent effect on cellular proliferation. However, growth factors in CE induced proteoglycan synthesis selectively in nonproliferating and expressing cells in confluent culture.

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