Effect of neem ( Azadirachta indica A. Juss) leaf extract on resistant Staphylococcus aureus biofilm formation and Schistosoma mansoni worms

Quelemes, Patrick Veras; Perfeito, Márcia Luana Gomes; Guimarães, Maria Adelaide; Santos, Raimunda Cardoso dos; Lima, David Fernandes; Nascimento, Carlos; Silva, Marcos P.N.; Soares, Maria José dos Santos; Röpke, Cristina Dislich; Eaton, Peter; Moraes, Josué de; Leite, José Roberto S. A.

doi:10.1016/j.jep.2015.09.026

Cited by 56 publications

(32 citation statements)

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“…Thus, A. indica, upon further investigation, could be developed into an alternative agent for the treatment of schistosomiasis. These results corroborate other studies elsewhere [23,24] and thus, demonstrate that A. indica could play an important role in any biological control program aimed at irradicating schistosomiasis. However, at low dose (< 500 μg/mL), dose response was not observed for N. latifolia, M. lucida and P. amarus.…”

Section: Discussionsupporting

confidence: 92%

Ethnopharmacological evaluation of schistosomicidal and cercaricidal activities of some selected medicinal plants from Ghana

et al. 2020

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Background: The adulticidal and cercaricidal activities of five Ghanaian medicinal plants, namely, Phyllanthus amarus, Vernonia amygdalina, Azadirachta indica, Morinda lucida and Nauclea latifolia against S. mansoni were evaluated in this study. Six weeks old ICR mice (n = 25) were percutaneously infected with S. mansoni cercariae. Nine weeks later, infected mice (n = 5) were anaesthetised and perfused for adult S. mansoni. Cercariae were treated with different concentrations (1000, 500, 250, 125, 62.5, 31.25 μg/mL) of methanolic extracts of the experimenting plants in triplicates. Adult S. mansoni incopula were also treated with same concentrations of each extract or 20 μg/mL praziquantel. The cercariae and adult worms were observed at time intervals for 180 min and 120 h to assess mortality and viability respectively. Additionally, 9-week cercariae-infected mice (4 groups of 5 mice) were treated with either 500 mg/kg po A. indica or V. amygdalina, 400 mg/kg po praziquantel or distilled water for 14 days. The mice were euthanized after adult worms were recovered from them. The liver was processed and histologically examined for granuloma formations. Results: All the plants exhibited varying cercaricidal and adulticidal activities against S. mansoni in a time and concentration-dependent manner. A. indica (3 h IC 50 = 27.62 μg/mL) and V. amygdalina (3 h IC 50 = 35.84 μg/mL) exerted the highest cercaricidal activity. Worm recovery after treatment with V. amygdalina, A. indica and praziquantel in vivo was 48.8%, 85.1 % and 59.9 % respectively (p < 0.05). A. indica and V. amydalina-treated mice recorded lesser mean liver and spleen weights compared to untreated groups (p < 0.05). Conclusion:A. indica demonstrated the highest cercaricidal and alduticidal activities in vitro, whereas V. amygdalina exhibited the most potent aldulticidal activity in vivo. This study could provide baseline information which can be used to develop plant-based alternative commercial drugs against S. mansoni.

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Section: Discussionsupporting

confidence: 92%

Ethnopharmacological evaluation of schistosomicidal and cercaricidal activities of some selected medicinal plants from Ghana

et al. 2020

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“…A total of 2% aqueous extract of Neem chewing sticks inhibited in vitro the growth of Staphylococci, Streptococci and E. coli [40]. Ethanolic extract of Neem leaf demonstrated inhibitory effect on Methicillin-resistant Staphylococcus aureus biofilm by decreasing its ability to form large clusters [41]. The methanolic extract of Neem proved robust antimicrobial activity against polymicrobial dentinal biofilms of common endodontic pathogens, such as Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis, and Candida albicans [42].…”

Section: Methodsmentioning

confidence: 99%

Functional Bioglass—Biopolymer Double Nanostructure for Natural Antimicrobial Drug Extracts Delivery

et al. 2020

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Aseptic loosening and periprosthetic infections are the main causes of implant failure. Strategies to mitigate this drawback are therefore mandatory to avoid primary and revision replacement surgeries. A functional bioapatite–biopolymer double nanostructure fabricated by matrix-assisted pulsed laser evaporation to prevent infection of orthopedic and dental implants could promote osseointegration and ensure controlled delivery of natural antimicrobial drugs. The synthesized nanostructure consists of two overlapping layers, the lower from a biocompatible polymer for anticorrosive protection, and the upper of bioactive glass incorporating antimicrobial plant extract, acting as a potential drug delivery system. Morphology, composition, adherence, ability for drug delivery and biological properties (cytotoxicity and antimicrobial effect) were studied. Structures proved compact and stable, conserving a remarkable drug delivery ability for more than 21 days, i.e., enough to ensure long-term microbes’ eradication.

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“…They have been applied as an anthelmintic for more than 50 y actually [12][13][14] . Studies showed that Szechwan Chinaberry extracts exhibited biological activities in blocking neuromuscular junctions, antibotulism, and affecting efferent nerves, central nervous and the respiratory system [15][16][17] . Recently, several experts and scholars pointed out that Szechwan Chinaberry extracts have antineoplastic effects, including inhibition to hepatocellular carcinoma cells, human leukemia cells and human breast cancer cells, by primarily inducing the apoptosis of relevant cells [18][19][20] .…”

Section: Effects Of Chinaberry Tree Extract On Proliferation Apoptosmentioning

confidence: 99%

Effects of Chinaberry Tree Extract on Proliferation, Apoptosis, Migration and Cell Cycle Distribution of Human Breast Cancer Cells

Xiao¹,

Chu²,

Hongyan³

et al. 2020

pharmaceutical-sciences

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Jian, et al.: Effects of Chinaberry Tree Extract on Breast Cancer Cells To study the effects of chinaberry tree extract on proliferation, apoptosis, migration and cell cycle distribution of human breast cancer cells, the MCF-7 breast cancer cells obtained were divided into blank group, low concentration group, medium concentration group and high concentration group. Phosphate buffered saline solution and sterile physiological saline were added to the blank group for cell culture. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry were used to detect cell proliferation and apoptosis at different time periods. Transwell assay was used to detect cell migration; flow cytometry was used to detect cell cycle distribution, and intercellular cell adhesion molecule-1, matrix metalloproteinase-2, matrix metalloproteinase-9 were detected by Western blotting. The proliferation rate of MCF-7 breast cancer cells in the low, medium and high concentration group was significantly lower than that in the blank group at different time periods, while the apoptosis rate was significantly higher than that in the blank group, and the effect was more obvious with the increase of concentration (p<0.05). The number of cell migration of MCF-7 breast cancer cells in the low, medium and high concentration group was significantly reduced compared with the blank group, and the cell migration was significantly reduced with the increase of the extract concentration of chinaberry tree (p<0.05). The higher the extract concentration of chinaberry tree, the lower the proportion of MCF-7 cells in G0/G1 and S cycle and the higher the proportion of MCF-7 cells in G2/M cycle (p<0.05). The expressions of intercellular cell adhesion molecule-1, matrix metalloproteinase-2 and matrix metalloproteinase-9 in the low, medium and high concentration groups were significantly lower than those in the blank group, and the expressions of intercellular cell adhesion molecule-1, matrix metalloproteinase-2 and matrix metalloproteinase-9 in the high concentration group were significantly lower than those in the low and medium concentration groups (p<0.05). The extract of chinaberry tree can effectively inhibit the proliferation and migration promote the apoptosis and reduce the growth of breast cancer cells by changing the periodic distribution of cancer cells, and its ability is concentration dependent.

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Effect of neem ( Azadirachta indica A. Juss) leaf extract on resistant Staphylococcus aureus biofilm formation and Schistosoma mansoni worms

Abstract: Neem leaf ethanolic extract presented inhibitory effect on MRSA biofilm and planktonic aggregation formation, and anthelmintic activity against S. mansoni worms.

Cited by 56 publications

References 55 publications

Ethnopharmacological evaluation of schistosomicidal and cercaricidal activities of some selected medicinal plants from Ghana

Ethnopharmacological evaluation of schistosomicidal and cercaricidal activities of some selected medicinal plants from Ghana

Functional Bioglass—Biopolymer Double Nanostructure for Natural Antimicrobial Drug Extracts Delivery

Effects of Chinaberry Tree Extract on Proliferation, Apoptosis, Migration and Cell Cycle Distribution of Human Breast Cancer Cells

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