Effect of nitrogen source on growth and morphogenesis of three micropropagated potato cultivars

Avila, A. de L.; Pereyra, Soledad; Collino, D. J.; Argüello, J. A.

doi:10.1007/bf02358717

Cited by 10 publications

(6 citation statements)

References 16 publications

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“…The nitrogen treatments (NO 3 :NH 4 =55:5mM and 50 mM KNO 3 : 10 mM NH 4 NO 3 ) followed chitosan treatments were preferable after chitosan treatments for number of node. Moreover, this result was parallel with Avila et al, (1994) who found that potato plantlets growth were less with NH 4 or NO 3 as a sole source of nitrogen and Villamor (2010) who indicated that nitrogen in the form of KNO 3 significantly improved proliferated rate of ginger in vitro. Also, Avila et al, (1994) found that decreasing proportion of NH 4 from 50% to 30 % increased shoot growth.…”

Section: Results and Discussion First Experiment: Microtuberization supporting

confidence: 80%

“…Moreover, this result was parallel with Avila et al, (1994) who found that potato plantlets growth were less with NH 4 or NO 3 as a sole source of nitrogen and Villamor (2010) who indicated that nitrogen in the form of KNO 3 significantly improved proliferated rate of ginger in vitro. Also, Avila et al, (1994) found that decreasing proportion of NH 4 from 50% to 30 % increased shoot growth. The application of NO 3 :NH 4 =55:5mM was the best treatment (5.0).…”

Section: Results and Discussion First Experiment: Microtuberization supporting

confidence: 80%

“…Potato plantlets growth was less with either NH 4 + or NO 3 as a sole nitrogen source (Avila et al, 1994). Both growth and morphogenesis in tissue culture are considerably influenced by the availability and form of nitrogen (George et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improving Production and Characters of Potato Microtubers and Minitubers

Wadod¹,

Aal²,

Ibrahim³

2017

Menoufia Journal of Plant Production

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Section: Results and Discussion First Experiment: Microtuberization supporting

confidence: 80%

Section: Results and Discussion First Experiment: Microtuberization supporting

confidence: 80%

See 1 more Smart Citation

Improving Production and Characters of Potato Microtubers and Minitubers

Wadod¹,

Aal²,

Ibrahim³

2017

Menoufia Journal of Plant Production

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“…A maioria dos trabalhos com batata in vitro baseia-se no uso de meios de cultura semi-sólidos (Caligari & Powell, 1989;Ávila et al, 1994). Entretanto, a utilização de meios de cultura líquidos tem proporcionado igual ou até maior eficiência para diversas espécies vegetais, como o abacaxi (Escalona et al, 1999;Feuser et al, 2001), a banana (Alvard et al, 1993;Levin et al, 1997) e a cana-de-açúcar (Lorenzo et al, 1998).…”

Section: Introductionunclassified

Protocolo para produção de material propagativo de batata em meio líquido

Pereira

Fortes

2003

Pesq. agropec. bras.

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Resumo -Os meios semi-sólidos são os mais utilizados em trabalhos de micropropagação, mas há indícios de que o estado físico dos meios de cultura influencia a multiplicação dos cultivos. O objetivo deste trabalho foi estabelecer um protocolo para a multiplicação de material propagativo de batata em meio de cultura líquido. Explantes de batata da cultivar Eliza, com uma gema axilar, foram cultivados em seis diferentes meios de cultura, com (semi-sólido) ou sem (líquido) a adição do solidificante ágar. Após 21 dias de cultivo, o meio de cultura que proporcionou os melhores resultados para crescimento e taxa de multiplicação teve sua composição modificada com o objetivo de melhorar a eficiência de multiplicação de cinco cultivares em meio líquido. Foi também avaliada a necessidade de agitação dos cultivos em meio líquido. Houve ganho na eficiência de multiplicação in vitro da batata, quando se utiliza meio de cultura líquido. Os melhores resultados são obtidos quando o material é cultivado em meio constituído pelos sais de MS na concentração plena, acrescido de ácido pantotênico (5,0 mg L -1 ), tiamina (1,0 mg L -1 ), ácido giberélico (0,25 mg L -1 ) e sacarose (20 g L -1 ), sob agitação constante.Termos para indexação: Solanum tuberosum, micropropagação, cultura in vitro, propagação vegetal. Protocol for potato propagative material production in liquid mediumAbstract -Semi-solid media are more commonly used in studies of micropropagation, but the physical state of the culture media seems to influence the growth and multiplication rate of the cultivation. The objective of this work was to establish a protocol for in vitro multiplication of potato in liquid culture media. Potato explants, cultivar Eliza, with an axillary bud, were cultivated in six different culture media, with (semi-solid) or without (liquid) the addition of agar. After 21 days, the culture medium that provided the best results for growth and multiplication rate had the composition modified with the objective of improving the efficiency of in vitro multiplication of five cultivars in liquid medium. The need of agitation was also evaluated in liquid culture medium. There was high efficiency of the in vitro potato multiplication when it was cultivated in liquid medium. The MS salt medium in the full concentration, added by gibberellic acid (0.25 mg L -1 ), panthotenic acid (5.0 mg L -1 ), thiamine (1.0 mg L -1 ) and sucrose (20 g L -1 ), and under constant agitation, showed to be suitable for this purpose.Index terms: Solanum tuberosum, micropropagation, in vitro culture, plant propagation.(1) Aceito para publicação em 24 IntroduçãoA técnica de cultivar tecidos de plantas in vitro vem sendo rotineiramente aplicada a inúmeras espécies vegetais, pela possibilidade de manutenção da identidade genética dos indivíduos e obtenção de grande número de plantas sadias e de alta qualidade em pequeno espaço físico e em curto tempo, independentemente da época do ano. Por sua vez, o uso comercial da cultura de tecidos é ainda limitado, principalmente pelo elevado...

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“…Among plant tissue culture techniques, micropropagation has the greatest practical application (reviewed in SHAHZAD et al, 2017). Several studies have been reported in the literature, aiming at the optimization of potato micropropagation protocols (ÁVILA et al, 1994;PEREIRA;FORTES, , 2004DUTRA et al, 2010;VENKATASALAM et al, 2013). However, one of the greatest difficulties in the development of these protocols is the in vitro establishment of plants, mainly due to fungal, viral and bacterial contaminations.…”

Section: In Vitro Establishmentmentioning

confidence: 99%

Application of tissue culture techniques in potato

Morais¹,

Asmar²,

Silva³

et al. 2018

Biosci. J.

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Potato is the world's most important non-cereal food crop, and therefore, it is considered one of the major food sources for humankind. Its conventional propagation is asexual, by using the tuber, which allows the accumulation and dissemination of pathogens to new cultivation areas. This fact not only impairs the yield of this solanaceous plant, but also threatens the maintenance of genotypes for commercial or breeding purposes. Due to the impossibility of using botanical seed, conservation and exchange of germplasm of this species by means of conventional methods are not feasible. In all potato-producing regions, the demand for high-quality tubers has been paramount to ensure crops production. Thus, biotechnological techniques based on tissue culture are very important. Plant tissue culture offers alternative methods of propagation by in vitro techniques that provide production and multiplication of material with high sanity. Thus, this literature review summarizes the history and current situation of tissue culture techniques applied to potato crop. Besides clonal multiplication, this biotechnological tool makes available initial indexed material to breeding programs and certified seed potato, and facilitates the exchange and conservation of germplasm. For all these reasons, the use of these techniques in potato production chain directly benefits producers by providing high-quality propagules.

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Effect of nitrogen source on growth and morphogenesis of three micropropagated potato cultivars

Cited by 10 publications

References 16 publications

Improving Production and Characters of Potato Microtubers and Minitubers

Improving Production and Characters of Potato Microtubers and Minitubers

Protocolo para produção de material propagativo de batata em meio líquido

Application of tissue culture techniques in potato

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