Effect of nitrogen sources on the regulation of extracellular lipase production in Acinetobacter calcoaceticus strains

Abstract

Extracellular lipase production and lipA expression were studied in A.calcoace~cus strains in relation to the nitrogen sources. Addition of casamino-acids or tryptone improved 2-3 times lipase yield as compared to ammonium, yeast extract or proteosepeptone. Further increase in lipase yield and enzyme stability were achieved when ammonium was also added. Differences in yields were not related to lipA transcription. Proteolytic activity was detected in culture broths simultaneous with lipase degradation. Post-transcriptional processes including enzyme protection, inactivation and secretion must be considered as important factors affecting lipase production.

Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology

“…Olive oil was the most effective compared to soybean oil, palm oil, canola oil and sunflower oil [14]. Besides, as a nitrogen source, tryptone is rich in minerals and ions which has also been reported to improve the lipase activity [17]. Tween-80 not only acted as an effective surfactant but also a lipase inducer [13].…”

Production, purification, characterization, and applications of lipases

“…This enzyme was stable to both hydrogen peroxide and alkaline protease . Cordenons et al (1996) examined various nitrogen sources for producing extracellular lipase from Acinetobacter calcoaceticus. Use of amino acids and tryptone improved the lipase yield by a factor of 2 or 3 when compared to the use of ammonium, yeast extract, and protease peptone (Cordenons et al, 1996).…”

“…Cordenons et al (1996) examined various nitrogen sources for producing extracellular lipase from Acinetobacter calcoaceticus. Use of amino acids and tryptone improved the lipase yield by a factor of 2 or 3 when compared to the use of ammonium, yeast extract, and protease peptone (Cordenons et al, 1996). However, lipase yield and stability could be improved by supplementing the preferred organic nitrogen source with ammonium (Cordenons et al, 1996).…”

“…Use of amino acids and tryptone improved the lipase yield by a factor of 2 or 3 when compared to the use of ammonium, yeast extract, and protease peptone (Cordenons et al, 1996). However, lipase yield and stability could be improved by supplementing the preferred organic nitrogen source with ammonium (Cordenons et al, 1996). The extracellular lipase was measured using pNPP as the substrate (Vorderwiilbecke et al, 1992).…”

Improvement of Spinosad Production upon Utilization of Oils and Manipulation of β-Oxidation in a High-Producing <b><i>Saccharopolyspora spinosa</i></b> Strain

“…Lipase activity could be detected in the broth at 24 h and it reached a maximum value at 120 h, after which the lipase activity declined until the end of the fermentation (Fig. 2f), which might be due to the proteolytic cleavage in the culture broths [Cordenons et al, 1996]. The lipase activity of ASAGF73 was produced constitutively at a low level (18.08 ± 1.25 U/mL at 120 h) in the absence of oil, while more activity seemed to be induced in the presence of oils.…”