Effect of nutrient availability on cylindrospermopsin gene expression and toxin production in Cylindrospermopsis raciborskii

Rigamonti, Natalia; Aubriot, Luis; Martigani, Fátima; Bonilla, Sylvia; Piccini, Claudia

doi:10.3354/ame01877

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…In our two co-culture experiments, CYN quotas in toxic R. raciborskii were higher under nutrient-replete conditions, consistent with that of Burford et al (2014), who found P-replete treatments had higher CYN quotas. Conversely, some studies showed that unfavorable conditions such as N or P limitation may trigger higher CYN production (Saker and Neilan, 2001;Bácsi et al, 2006;Rigamonti et al, 2018;Yang et al, 2018;Nor et al, 2019). Our results were also different from several recent studies suggesting that CYN production was constitutive metabolic processes in toxic R. raciborskii and not affected by different light, CO 2 and nutrient conditions (Pierangelini et al, 2015;Willis et al, 2015;Yang et al, 2017).…”

Section: Discussioncontrasting

confidence: 99%

“…This phenomenon also could be explained by the allelopathy effect (Briand et al, 2008;Lei et al, 2015). Some previous studies showed that CYN may act as allelopathic substances to inhibit the growth of other competitors and assist in nutrient uptake, allowing the CYN producers to outcompete sympatric species in environments (Bar-Yosef et al, 2010;B-Béres et al, 2012;Chia et al, 2017;Rigamonti et al, 2018). Our findings of a coincidence in increased CYN synthesis and competitive advantage of toxic strains support that CYN production may be a functional strategy for competition with other phytoplankters.…”

Section: Discussionsupporting

confidence: 79%

“…The ability to fix atmospheric nitrogen has been proposed as a mechanism that allows R. raciborskii to bloom in nitrogenlimited freshwater bodies (Burford et al, 2016). For the strain CYP011K of R. raciborskii, nitrogen fixation has made it to grow with a higher growth rate under the combination of nitrate deprivation and phosphate repletion (Rigamonti et al, 2018). However, it seemed that N 2 fixation can't supply sufficient nitrogen for persistent growth of R. raciborskii under N-limited (N2 and N3) conditions as compare to N-replete conditions (control and N1) in our monoculture and co-culture experiments.…”

Section: Discussionmentioning

confidence: 99%

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Nutrient Regulation of Relative Dominance of Cylindrospermopsin-Producing and Non-cylindrospermopsin-Producing Raphidiopsis raciborskii

Lei

Cheng

et al. 2021

Front. Microbiol.

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Raphidiopsis raciborskii (previously Cylindrospermopsis raciborskii) can produce cylindrospermopsin (CYN) which is of great concern due to its considerable toxicity to human and animals. Its CYN-producing (toxic) and non-CYN-producing (non-toxic) strains co-exist commonly in natural water bodies, while how their relative dominance is regulated has not been addressed. In this study, we combined field investigation with laboratory experiments to assessed the relationship between toxic and non-toxic R. raciborskii abundances under different nutrient levels. The rpoC1- and cyrJ-based qPCR was applied for quantifying total and toxic R. raciborskii abundances, respectively. The field survey showed that toxic R. raciborskii was detected in 97 of 115 reservoirs where its proportion ranged from 0.3% to 39.7% within the R. raciborskii population. Both total and toxic R. raciborskii abundances increased significantly with trophic level of these reservoirs, consistent with our monoculture and co-culture experiments showing in an increase in R. raciborskii growth with increasing nitrogen (N) or phosphorus (P) concentrations. In the monoculture experiments, growth rates of non-toxic and toxic strains from Australia or China were not significantly different under the same culture conditions. On the other hand, in the co-culture experiments, the toxic strains displayed a significantly faster growth than non-toxic strains under nutrient-replete conditions, resulting in an obvious shift toward the dominance by toxic strains from day 3 to the end of the experiments, regardless of the strain originating from Australia or China. The reverse was found under N- or P-limited conditions. Our results indicated that the toxic strains of R. raciborskii have a competitive advantage relative to the non-toxic strains in a more eutrophic world. In parallel to an increase in dominance, both toxic strains grown in the mixed population significantly increased CYN production under nutrient-replete conditions as compared to nutrient-limited conditions, suggesting that CYN may be of significance for ecological advantage of toxic R. raciborskii. These results highlight the importance of nutrient availability in regulating abundances and strain dominance of two genotypes of R. raciborskii. Our findings demonstrated that elevated nutrients would favor the growth of CYN-producing R. raciborskii and CYN production, leading to more blooms with higher toxicity at global scale.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

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Nutrient Regulation of Relative Dominance of Cylindrospermopsin-Producing and Non-cylindrospermopsin-Producing Raphidiopsis raciborskii

Lei

Cheng

et al. 2021

Front. Microbiol.

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“…However, primer optimization for multiplex PCR is laborious and is sometimes prone to cross-oligo contamination. Several other qPCR and their variant methods are developed for the detection of STXs [95,96], CYNs [97], and ATXs [98]. Recently, the loop-mediated isothermal amplification (LAMP) method was developed, which amplifies the target gene with an optimum temperature ranging from 60 to 65°C.…”

Section: Molecular Methodsmentioning

confidence: 99%

Recent trends in the detection of freshwater cyanotoxins with a critical note on their occurrence in Asia

Kulabhusan

Campbell

2021

Trends in Environmental Analytical Chemistry

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Overview of the distribution and adaptation of a bloom-forming cyanobacterium Raphidiopsis raciborskii: integrating genomics, toxicity, and ecophysiology

Yang

Shi

2022

J. Ocean. Limnol.

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Effect of nutrient availability on cylindrospermopsin gene expression and toxin production in Cylindrospermopsis raciborskii

Cited by 5 publications

References 27 publications

Nutrient Regulation of Relative Dominance of Cylindrospermopsin-Producing and Non-cylindrospermopsin-Producing Raphidiopsis raciborskii

Nutrient Regulation of Relative Dominance of Cylindrospermopsin-Producing and Non-cylindrospermopsin-Producing Raphidiopsis raciborskii

Recent trends in the detection of freshwater cyanotoxins with a critical note on their occurrence in Asia

Overview of the distribution and adaptation of a bloom-forming cyanobacterium Raphidiopsis raciborskii: integrating genomics, toxicity, and ecophysiology

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