2017
DOI: 10.1016/j.theriogenology.2017.03.006
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Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas

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Cited by 15 publications
(24 citation statements)
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“…These results confirmed that swim-up is not an adequate method of sperm selection in llamas, even after collagenase treatment. However, this technique gave acceptable results when used on alpaca epididymal sperm ( 12 ). These authors did not report the number of motile spermatozoa recovered, but they obtained embryos by IVF using swim-up in alpaca epididymal sperm.…”
Section: In Vitro Embryo Productionmentioning
confidence: 99%
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“…These results confirmed that swim-up is not an adequate method of sperm selection in llamas, even after collagenase treatment. However, this technique gave acceptable results when used on alpaca epididymal sperm ( 12 ). These authors did not report the number of motile spermatozoa recovered, but they obtained embryos by IVF using swim-up in alpaca epididymal sperm.…”
Section: In Vitro Embryo Productionmentioning
confidence: 99%
“…This gamete can be collected from dead or live animals. The methods used to obtain cumulus-oocyte complexes (COCs) are: aspiration or dissection of follicles from slaughterhouse ovaries ( 9 12 ); aspiration of follicles surgically exposed during a laparotomy [llamas ( 13 – 16 ); alpacas ( 17 )] or ultrasound-guided transvaginal aspiration of follicles ( 18 21 ).…”
Section: In Vitro Embryo Productionmentioning
confidence: 99%
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“…This species possesses interesting physiological and reproductive characteristics such as induced ovulation (Bravo et al, ) and the presence, in the male seminal plasma, of an ovulation‐inducing factor (OIF; Kershaw‐Young and Maxwell, ; Kershaw‐Young et al, ; Stuart et al, ). For these reasons, past research has been mainly focused on reproductive mechanisms (Bravo et al, ; Correa et al, ; Ratto et al, , , ; Ruiz et al, ). The study of early morphogenesis in Vicugna pacos became more important after the introduction of reproductive techniques such as in vitro embryo production (Trasorras et al, ).…”
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confidence: 99%