Effect of organic loading rate and solids retention time on microbial population during bio-hydrogen production by dark fermentation in large lab-scale

Mariakakis, Iosif; Bischoff, Pierre; Krampe, Jörg; Meyer, Carsten; Steinmetz, Heidrun

doi:10.1016/j.ijhydene.2011.06.008

Cited by 53 publications

(48 citation statements)

References 40 publications

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“…Methanogenesis could be inhibited through the thermal pre-treatment of the seed sludge and the selected operation parameters were sufficient for hindering the proliferation of the methanogens in the system. In our previous work for which no pre-treatment of the seed sludge was carried out methanogenesis could be only be inhibited for 120 d (Mariakakis et al, 2011). Sucrose in higher concentrations could be detected only in phases D2 and D7 resulting in average substrate degradations of 71.5% and 83.1% respectively.…”

Section: Reactor Operationmentioning

confidence: 82%

“…DNA extraction, 16S rDNA of eubacteria and clostridia PCR amplification, DGGE analysis and sequencing were performed as previously described (Mariakakis et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

“…Such an enhancing symbiosis can not be excluded that also takes place in the system of this work, so that the residual dissolved oxygen of the water added into the system could be removed. More over, the possibility of a secondary fermentation by C. tyrobutyricum present in most of the experimental phases and facilitated by the production of lactate by the LAB as described in our previous work (Mariakakis et al, 2011) and later demonstrated (Wu et al, 2012) has to be considered, too.…”

Section: Symbioses In Fermentative Hydrogen Production Systemsmentioning

confidence: 95%

“…Upon sucrose depletion continuous operation of the system was started. The pH was reduced to 5.5, a value that has been reported to be the optimum for continuous bio-hydrogen production (Mariakakis et al, 2011). The first phase of continuous operation aimed at the further selection of the biomass for hydrogen producing bacteria by application of high HRT and low OLR.…”

Section: Reactor Operationmentioning

confidence: 99%

See 3 more Smart Citations

Fermentative Hydrogen Production by Molasses; Effect of Hydraulic Retention Time, Organic Loading Rate and Microbial Dynamics

Mariakakis¹,

Meyer²,

Steinmetz³

2012

Hydrogen Energy - Challenges and Perspectives

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Section: Reactor Operationmentioning

confidence: 82%

“…DNA extraction, 16S rDNA of eubacteria and clostridia PCR amplification, DGGE analysis and sequencing were performed as previously described (Mariakakis et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Section: Symbioses In Fermentative Hydrogen Production Systemsmentioning

confidence: 95%

Section: Reactor Operationmentioning

confidence: 99%

See 2 more Smart Citations

Fermentative Hydrogen Production by Molasses; Effect of Hydraulic Retention Time, Organic Loading Rate and Microbial Dynamics

Mariakakis¹,

Meyer²,

Steinmetz³

2012

Hydrogen Energy - Challenges and Perspectives

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“…Some studies had revealed that there are several operational parameters that significantly affect the performance of anaerobic digestion process including pH, temperature, hydraulic retention time (HRT), organic loading rate, solid concentration and reactor configuration (Rincón et al 2008;Mariakakis et al 2011;Bayr et al 2012;Nasir et al 2012;Darwin et al 2016). The HRT is considered as the most crucial parameter that extremely affects the performance of anaerobic digestion process, particularly microbial activities involved in the different stages of the process (Fang et al 2000;Kim et al 2006).…”

mentioning

confidence: 99%

Kinetics on anaerobic co-digestion of bagasse and digested cow manure with short hydraulic retention time

Darwin¹,

Fazil²,

Ilham³

et al. 2017

Res. Agric. Eng.

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Darwin, Fazil A., Ilham M., Sarbaini, Purwanto S. (2017): Kinetics on anaerobic co-digestion of bagasse and digested cow manure with short hydraulic retention time. Res. Agr. Eng., 63: 121-127.The anaerobic co-digestion of bagasse with digested cow manure was operated in 3 l semi-continuous reactor under mesophlic temperature at 34 ± 1°C. Short hydraulic retention time and high organic loading rate applied were 10 days and 3.465 kg volatile solids (VS)/m 3 .day, respectively. Anaerobic co-digestion of bagasse with digested cow manure obtained higher biogas yield (69 ml/g VS) compared with the anaerobic digestion of digested cow manure alone (20.42 ml/g.VS). Kinetic assessment revealed that the maximum specific growth rate, the maximum rate of substrate consumption, halfvelocity constant, endogenous decay constant and microbial growth yield obtained were 3.917 day -1 , 870.309 mg/mg, 15.09 mg/l , 8.1518 day -1 and 0.0193 mg/mg, respectively. This result indicated that a longer retention time was required to allow the bacterial growth.

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Key factors for biohydrogen production by dark fermentation

et al. 2014

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Among biological ways of hydrogen production, the dark fermentation process gives important prospects. One of the key factors of bioreactor operation is the biogas extraction. Thus, this work gives a parametric optimization of the bioreactor as a function of the sweep gas nature and flow rate. The bacterial inoculum was activated sludge of wastewater treatment plant, which was thermally treated to inhibit the activity of hydrogenotrophic and non‐hydrogen producing micro‐organisms. The fermentation was performed in a semi‐batch bioreactor with a model substrate. Biogas was analyzed online by GC‐TCD and metabolites (volatile fatty acids and alcohols) were analyzed by GC‐FID. Kinetics parameters of hydrogen production were obtained by modelling. Increase the sweep gas (N2) flow rate in the bioreactor results in an increase of hydrogen yield and cumulative production of 35 %. In the purpose of recycling the CO2 produced by fermentation, the addition of CO2 to N2 as sweep gas gives a yield improvement from 2.10 molH2/molhexose with N2 alone, to 2.37 molH2/molhexose with a mixture of CO2:N2 (75:25). In order to reduce the amount of suspended matter in initial biomass, filtration tests show an equivalent hydrogen yield but with a higher lag time than with unfiltered biomass (30 h towards 4.4 h). The presence of hydrogen producing bacteria mainly stick with the solid part of sludge was highlighted.

show abstract

Effect of organic loading rate and solids retention time on microbial population during bio-hydrogen production by dark fermentation in large lab-scale

Cited by 53 publications

References 40 publications

Fermentative Hydrogen Production by Molasses; Effect of Hydraulic Retention Time, Organic Loading Rate and Microbial Dynamics

Fermentative Hydrogen Production by Molasses; Effect of Hydraulic Retention Time, Organic Loading Rate and Microbial Dynamics

Kinetics on anaerobic co-digestion of bagasse and digested cow manure with short hydraulic retention time

Key factors for biohydrogen production by dark fermentation

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