Effect of Ouabain on Cochlear Potentials and Endolymph Composition in Guinea Pigs

Konishi, T.; Mendelsohn, M.

doi:10.3109/00016487009123353

Cited by 114 publications

(50 citation statements)

References 16 publications

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“…Perilymphatic perfusion of ouabain, an inhibitor of the NKAα, causes a rapid reduction of the endocochlear potential (Konishi and Mendelsohn 1970;Kuijpers and Bonting 1970;Kusakari et al 1978), and researchers have identified NKAα1 in the strial marginal cells and NKAα2 in the lateral wall fibrocytes (McGuirt and Schulte 1994;Schulte and Steel 1994; reviewed in Wangemann 2006). The contribution of the NKAα1 and NKAα2 in maintaining the endocochlear potential is supported by the characterization of mice carrying deletions of these subunits.…”

Section: Discussionmentioning

confidence: 99%

“…Because inhibitors of the NKAα are known to reduce the endocochlear potential (Konishi and Mendelsohn 1970), previous research investigating the localization of the NKAα in the cochlea has focused on its expression in structures, like the stria vascularis and spiral ligament, responsible for maintaining the endocochlear potential (Wangemann 2006). Nonetheless, earlier work also reported expression of the NKAα in the neuronal elements of the mammalian cochlea using a variety of techniques, including enzyme cytochemistry (Kerr et al 1982), in situ hybridization (Ryan and Watts 1991), and immunohistochemistry (Schulte and Adams 1989;Iwano et al 1990;McGuirt and Schulte 1994;Schulte and Steel 1994;ten Cate et al 1994;Nakazawa et al 1995;Zuo et al 1995;Erichsen et al 1996;Peters et al 2001;Weber et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Distribution of the Na,K-ATPase α Subunit in the Rat Spiral Ganglion and Organ of Corti

McLean

Smith

Glowatzki

et al. 2008

JARO

107

105

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Processing of sound in the cochlea involves both afferent and efferent innervation. The Na,K-ATPase (NKA) is essential for cells that maintain hyperpolarized membrane potentials and sodium and potassium concentration gradients. Heterogeneity of NKA subunit expression is one mechanism that tailors physiology to particular cellular demands. Therefore, to provide insight into molecular differences that distinguish the various innervation pathways in the cochlea, we performed a variety of double labeling experiments with antibodies against three of the α isoforms of the NKA (NKAα1-3) and markers identifying particular subsets of neurons or supporting cells in whole mount preparations of the organ of Corti and spiral ganglion. We found that the NKAα3 is abundantly expressed within the membranes of the spiral ganglion somata, the type I afferent terminals contacting the inner hair cells, and the medial efferent terminals contacting the outer hair cells. We also found expression of the NKAα1 in the supporting cells that neighbor the inner hair cells and express the glutamate transporter GLAST. These findings suggest that both the NKAα1 and NKAα3 are poised to play an essential role in the regulation of the type I afferent synapses, the medial efferent synapses, and also glutamate transport from the afferent-inner hair cell synapse.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Distribution of the Na,K-ATPase α Subunit in the Rat Spiral Ganglion and Organ of Corti

McLean

Smith

Glowatzki

et al. 2008

JARO

107

105

View full text Add to dashboard Cite

show abstract

“…Early metabolic studies demonstrated that the electrogenic mechanism is closely associated with both EP and cochlear cation gradients (Konishi and Mendelsohn, 1970;Kuijpers and Bonting, 1970;Johnstone, 1965;Davis et al, 1955) and described high Na,K-ATPase activity in stria vascularis and spiral ligament (Kuijpers and Bonting, 1969;Iinuma, 1967;Kuijpers et al, 1967 29425. for the a3-and P1-subunit isoforms. Subpopulations of fibroqtes in the spiral prominence, suprastrial and supralimbal regions, and vestibular system expressed either the al-or a2-isoform, or both.…”

Section: Introductionmentioning

confidence: 99%

Distribution of immunoreactive alpha- and beta-subunit isoforms of Na,K-ATPase in the gerbil inner ear.

McGuirt¹,

Schulte²

1994

J Histochem Cytochem.

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(3A3171).Biochemical and histochemical studies have demonstrated abundant Na,K-ATPase in the inner ear and provided new information concerning the ion transport capcities of specialized cell types. To extend these earlier observations, we immunostained inner ears from adult gerbils with antibodies specific for the three known a-and the two known P-isoforms of Na,K-ATPase. Merent inner ear cell types contained specific and distinct combinations of a-and P-subunit isoforms. Strial marginal cells and vestibular dark cells expressed the al-and P2-isoforms, whereas other positive epithelial cells e x p r d a1 in combination with Pl. Ganglion neurons and their peripheral processes showed positive immunostaining

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“…Various ion transporters and channels that may be responsible for the generation of EP and endolymphatic high [K ϩ ] exist in these cells. Na ϩ ,K ϩ -ATPase and Na ϩ ,K ϩ ,2Cl Ϫ cotransporter that are localized at the basolateral membrane of marginal cells contribute to the generation of EP, because ouabain and furosemide, specific blockers of Na ϩ ,K ϩ -ATPase and Na ϩ ,K ϩ ,2Cl Ϫ cotransporter, respectively, depressed EP (Kusakari et al, 1978;Konishi and Mendelsohn, 1970). In vestibule, on the other hand, endovestibular fluid also contains 150 mM K ϩ , but its potential is ϳ0 mV, although dark cells, corresponding to cochlear marginal cells, also express both Na ϩ ,K ϩ -ATPase and Na ϩ ,K ϩ ,2Cl Ϫ cotransporter at their basolateral membrane (Schulte and Adams, 1989;Marcus et al, 1994).…”

mentioning

confidence: 99%

An ATP-Dependent Inwardly Rectifying Potassium Channel, K_AB-2 (Kir4.1), in Cochlear Stria Vascularis of Inner Ear: Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential

Horio²,

et al. 1997

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Cochlear endolymph has a highly positive potential of approximately +80 mV. This so-called endocochlear potential (EP) is essential for hearing. Although pivotal roles of K+channels in the formation of EP have been suggested, the types and distribution of K+channels in cochlea have not been characterized. Because EP was depressed by vascular perfusion of Ba2+, an inhibitor of inwardly rectifying K+(Kir) channels, but not by either 4-aminopyridine or tetraethylammonium, we examined the expression of Kir channel subunits in cochlear stria vascularis, the tissue that is supposed to play the central role in the generation of positive EP. Of 11 members of the Kir channel family examined with reverse transcription-PCR, we could detect only expression of KAB-2 (Kir4.1) mRNA in stria vascularis. KAB-2 immunoreactivity was specifically localized at the basolateral membrane of marginal cells but not in either basal or intermediate cells. Developmental expression of KAB-2 in marginal cells paralleled formation of EP. Furthermore, deaf mutant mice (viable dominant spotting; WV/WV) expressed no KAB-2 in their marginal cells. These results suggest that KAB-2 in marginal cells may be critically involved in the generation of positive EP.

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Effect of Ouabain on Cochlear Potentials and Endolymph Composition in Guinea Pigs

Cited by 114 publications

References 16 publications

Distribution of the Na,K-ATPase α Subunit in the Rat Spiral Ganglion and Organ of Corti

Distribution of the Na,K-ATPase α Subunit in the Rat Spiral Ganglion and Organ of Corti

Distribution of immunoreactive alpha- and beta-subunit isoforms of Na,K-ATPase in the gerbil inner ear.

An ATP-Dependent Inwardly Rectifying Potassium Channel, K_AB-2 (Kir4.1), in Cochlear Stria Vascularis of Inner Ear: Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential

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Effect of Ouabain on Cochlear Potentials and Endolymph Composition in Guinea Pigs

Cited by 114 publications

References 16 publications

Distribution of the Na,K-ATPase α Subunit in the Rat Spiral Ganglion and Organ of Corti

Distribution of the Na,K-ATPase α Subunit in the Rat Spiral Ganglion and Organ of Corti

Distribution of immunoreactive alpha- and beta-subunit isoforms of Na,K-ATPase in the gerbil inner ear.

An ATP-Dependent Inwardly Rectifying Potassium Channel, KAB-2 (Kir4.1), in Cochlear Stria Vascularis of Inner Ear: Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential

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An ATP-Dependent Inwardly Rectifying Potassium Channel, K_AB-2 (Kir4.1), in Cochlear Stria Vascularis of Inner Ear: Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential