Effect of Oxygen on Host Cell Reactivation in
            <i>Bacteroides fragilis</i>

Slade, Heide J.K.; Schumann, J. Petra; Parker, John; Jones, David T.; Woods, David R.

doi:10.1128/jb.153.3.1545-1547.1983

Cited by 13 publications

(8 citation statements)

References 12 publications

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“…The studies were carried out on B. fragilis strain (Bf-2) and phage b-1 which have been described previously [2,[5][6][7][8]. Bacteria were propagated at 37°C with brain-heart infusion broth and agar [9].…”

Section: Bacteria Phage and Mediamentioning

confidence: 99%

“…The survival of phage b-1 irradiated with a Fluotest Piccolo 254 UV lamp was determined as described previously [2]. B. fragilis cells were treated with 0 to 0.0025% H202 before plating with irradiated phage.…”

Section: Phage and Cell Survivalmentioning

confidence: 99%

“…The ability of H202, 02 and UV to induce phage reactivation in exponential and stationary phase B. fragilis cells was compared. The experimental system for the induction of phage reactivation by O 2 has been described previously [2]. UV reactivation of phage b-1 was determined under anaerobic conditions in ceils which were exposed to increasing fluences of UV radiation (0 to 300 J/m2).…”

Section: Phage and Cell Survivalmentioning

confidence: 99%

“…Phage reactivation has been extensively used to study DNA repair systems in aerobic bacteria [1] but little is known about DNA repair processes in obligate anaerobes. We recently reported an O2-inducible phage reactivation system in the obligate anaerobe Bacteroides fragilis [2]. Since cellular metabolism * To whom correspondence should be addressed.The studies were carried out on B. fragilis strain (Bf-2) and phage b-1 which have been described previously [2,[5][6][7][8].…”

mentioning

confidence: 99%

“…We recently reported an O2-inducible phage reactivation system in the obligate anaerobe Bacteroides fragilis [2]. Since cellular metabolism * To whom correspondence should be addressed.The studies were carried out on B. fragilis strain (Bf-2) and phage b-1 which have been described previously [2,[5][6][7][8]. Bacteria were propagated at 37°C with brain-heart infusion broth and agar [9].…”

mentioning

confidence: 99%

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Peroxide inducible phage reactivation inBacteroides fragilis

Slade

Schumann

Jones

et al. 1983

FEMS Microbiology Letters

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Reactivation of UV‐irradiated phage b‐1 was induced by H2O2 and UV in Bacteroides fragilis. The characteristics of H2O2 and UV induced phage reactivation differ from a previously reported oxygen induced reactivation system. The survival of B. fragilis cells after UV irradiation was also increased by pretreatment with H2O2. DNA synthesis was not inhibited in the host cells exposed to H2O2 concentrations which induced phage reactivation. The pattern of DNA degradation and synthesis after UV irradiation with and without H2O2 differed from the effect of O2 on DNA synthesis in irradiated B. fragilis cells.

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Section: Bacteria Phage and Mediamentioning

confidence: 99%

Section: Phage and Cell Survivalmentioning

confidence: 99%

Section: Phage and Cell Survivalmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Peroxide inducible phage reactivation inBacteroides fragilis

Slade

Schumann

Jones

et al. 1983

FEMS Microbiology Letters

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Heat shock stress in Bacteroides fragilis

1985

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The response to heat shock was investigated in the obligate anaerobe Bacteroides fragilis. The cells responded quickly to stress and synthesised seven heat shock proteins immediately upon exposure to heat. The apparent molecular weights of the seven proteins differed from the apparent molecular weights of the proteins induced by UV irradiation, O2 and H2O2. Heat shock did not induce phage reactivation whereas UV irradiation, O2 and H2O2 did induce phage reactivation systems. Ethanol did not elicit the heat shock response. Two heat resistant B. fragilis mutants were isolated. Both mutants lost the ability to synthesise the same two heat shock proteins. It is concluded that the heat shock response and the responses to UV irradiation, O2 and H2O2 represent two independent groups of stress responses in B. fragilis.

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