Disinfection of dentures and resilient denture liners were preferred for cross-contamination treatment and better safety. Nevertheless, it has been found that these techniques affect the physical and mechanical properties of denture liners. Objective: To evaluate the impact of various disinfection methods on the tensile bond strength of acrylic soft liner. Material and Methods: 25 samples were made, then divided randomly into five groups (4 disinfection groups as well as a control group), after the daily disinfection procedure, distilled water was used to store the samples at room temperature. After 30 days, a universal testing device (Gester, China) was used to assess the tens ile bond strength. The machine applied force to the specim ens at a crosshead constant speed of 5 mm/minute until failure was recorded. Statistics software (SPSS) was used to analyze the measurements. Results: After 30 days of regular ex posure to different disinfection methods, a greater mean value for bond strength showed in the control group while the lesse r value was in the chlorhexidine group. No significant difference was found between the control and Protefix tablet groups. The sodium hypochlorite and microwave groups had no significant difference between them, but they were significantly different from the control, Protefix tablet, and chlorhexidine groups. the chlorhexidine group was significantly different from the control and other disinfected groups. Conclusion: All disinfected soft liners revealed a decrease i n tensile bond strength, which was not significant with the protefix cleansing tablet but significant with the chlorhexidine, sodium hypochlorite, and microwave regimes.