Effect of Partial Shading on Photoproduction of Hydrogen by Chlorella

Kojima, Eiichi; Lin, Bin

doi:10.1263/jbb.97.317

Cited by 6 publications

(6 citation statements)

References 18 publications

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“…Specifically, lipases show less catalytic rate when double bonds are located nearest the carboxyl group, such as DHA. [46][47][48] The chemical structure of DHA implies a difficulty for lipases into the FAEEs incorporation, and therefore transesterification reaction rendered minor product yields in comparison with EPA-EEs. Moreover, taking into consideration that the DHA-EEs used in the present study contain a high purity of 87% DHA, the production of STAGs become a difficult task.…”

Section: Optimization Of Ratio 2-mags To Omega-3 Faees For Stags Prodmentioning

confidence: 99%

Strategies for Enzymatic Synthesis of Omega‐3 Structured Triacylglycerols from Camelina sativa Oil Enriched in EPA and DHA

Castejón

Señoráns

2019

Euro J Lipid Sci & Tech

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Enzymatic production of enriched eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) structured triacylglycerols (STAGs) is done in a two‐step reaction from a new source of omega‐3 α‐linolenic acid (ALA), Camelina sativa oil. A fast‐pressurized liquid extraction method with ethyl acetate as green solvent is used to produce camelina oil with a 39.3% yield in less than 20 min. In a first stage, camelina oil with a 29.7% of ALA is used to enzymatically produce 2‐monoacylglycerols (2‐MAGs) with high ALA composition in position sn‐2 (49.8% of ALA) using Lipozyme TL IM at 25 °C. For omega‐3 incorporation into STAGs, several lipases are studied, with 2‐MAG and omega‐3 FAEEs at different ratios (1:5, 1:10, and 1:20 (w/w)), 10% (w/w) of biocatalyst at 35 °C. STAGs yields reached with EPA ethyl esters are notably higher than using DHA‐EE. Lipase from Candida antarctica B (CAL‐B) and Thermomyces lanuginosus (TLL) incorporate EPA into STAGs in the same degree, 95.1 and 95.5%, respectively. However, for enriched DHA STAGs, maximum reaction yield is reached with CAL‐B (81.0%). The transesterification method of 2‐MAGs from vegetable omega‐3 sources like camelina oil, with marine EPA and DHA FAEEs, opens new approaches for developing VLC‐PUFA fortified functional foods. Practical Applications: Results provided in this research not only demonstrate the efficiency of this enzymatic method for enriching camelina oil in EPA and DHA, but offer a very effective strategy for the development of omega‐3‐enriched products from other vegetable oil sources like chia or echium as ingredients for functional foods with recognized health claims for applications in nutraceutical and food industries. Camelina oil proved to be an excellent source to enzymatically produce 2‐MAGs enriched in omega‐3 ALA. Moreover, lipase screening with commercial biocatalysts establish the most appropriate strategy to reach maximum STAGs yields with direct application in industry. Tested lipases exhibit different degree of substrate specificity toward EPA and DHA, omega‐3 acids not present in natural seed oils. Thus, CAL‐B seems to be the most suitable lipase to produce high content EPA and DHA enriched STAGs from camelina oil as new food ingredients for nutraceutical applications. Enzymatic production of structured TAGs enriched in EPA and DHA is done in a two‐step reaction from a natural source of omega‐3 ALA, camelina oil, studying reaction kinetics and characterizing isolated products.

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Section: Optimization Of Ratio 2-mags To Omega-3 Faees For Stags Prodmentioning

confidence: 99%

Strategies for Enzymatic Synthesis of Omega‐3 Structured Triacylglycerols from Camelina sativa Oil Enriched in EPA and DHA

Castejón

Señoráns

2019

Euro J Lipid Sci & Tech

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“…This makes experiments under well-defined conditions in devoted lab-scale photobioreactors of primary interest, especially in order to in-depth investigate the high coupling of interacting transient intracellular mechanisms with extracellular conditions (medium composition, light, etc.). Examples of such kind of processes can be found in the literature, either in the general context of biomass growth [8][9][10][11] dedicated to the particular case of H 2 production [12][13][14][15][16][17]. The latter systems have confirmed the high dependence of the biological response on the cells environment under H 2 producing conditions, with a marked dynamic behavior evolving all along the process.…”

Section: Introductionmentioning

confidence: 99%

Investigation of H2 production using the green microalga Chlamydomonas reinhardtii in a fully controlled photobioreactor fitted with on-line gas analysis

Fouchard

Pruvost

Degrenne

et al. 2008

International Journal of Hydrogen Energy

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Chlamydomonas reinhardtii is able to produce molecular hydrogen in a clean way. Overall H 2 release is the result of complex, interacting and transient intracellular mechanisms. To relate the dynamic coupling between culture conditions and biological responses, an original lab-scale setup has been developed. Such device enables culture conditions to be highly controlled and provides on-line mass-spectrometric measurement of gas production. A first validation was conducted with the well-known protocol of sulfur deprivation. Biochemical analysis combined with gas flow control enabled instantaneous productivities to be calculated, and kinetic evolutions of successive physiological states to be obtained. An energetic study was also conducted. A maximal energetic yield of light conversion to H 2 energy of 0.125% was achieved, far from the photosynthesis potential and usual photobioreactor efficiencies reported for biomass application (around 10%). However, the designed photobioreactor connected with data acquisition system is an innovative tool for future methodical optimization of H 2 production using photosynthetic microorganisms, integrating both bioprocess and physiological aspects.

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“…Later, the same research group discovered that partial shading treatment in C. pyrenoidosa cultures with Na 2 O 4 S 2 could significantly increase the H 2 production. [ 50 ]…”

Section: Solar‐h2 With Natural Whole‐cell Biocatalystmentioning

confidence: 99%

Advances in Whole‐Cell Photobiological Hydrogen Production

Chen

Wang

et al. 2021

Advanced NanoBiomed Research

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Solar energy is the largest energy source on Earth. In contrast to the limited and greenhouse gases‐emitting fossil fuels, solar energy is inexhaustible, carbon neutral, and nonpolluting. The conversion of this most abundant but highly diffused source into hydrogen is increasingly attractive. In nature, photosynthetic microorganisms exploit solar energy to produce hydrogen via photosynthesis, which is also known as photobiological hydrogen production. More recently, various types of artificial materials have been developed to hybrid microorganisms for converting solar energy into hydrogen, namely, semiartificial photosynthesis hydrogen production. Herein, the strategies for converting solar energy into hydrogen with whole‐cell biocatalyst are summarized and their potentials for future social sustainable development are discussed.

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Effect of Partial Shading on Photoproduction of Hydrogen by Chlorella

Cited by 6 publications

References 18 publications

Strategies for Enzymatic Synthesis of Omega‐3 Structured Triacylglycerols from Camelina sativa Oil Enriched in EPA and DHA

Strategies for Enzymatic Synthesis of Omega‐3 Structured Triacylglycerols from Camelina sativa Oil Enriched in EPA and DHA

Investigation of H2 production using the green microalga Chlamydomonas reinhardtii in a fully controlled photobioreactor fitted with on-line gas analysis

Advances in Whole‐Cell Photobiological Hydrogen Production

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