Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers

Lehn, Reid C. Van; Atukorale, Prabhani U.; Carney, Randy P.; Yu, Yingmin; Stellacci, Francesco; Irvine, Darrell J.; Alexander‐Katz, Alfredo

doi:10.1021/nl401365n

Cited by 249 publications

(398 citation statements)

References 49 publications

Supporting

Mentioning

352

Contrasting

Unclassified

Order By: Relevance

“…49 However, in very recent work, computational modeling in parallel with experimental studies using model membranes suggested that these amph-AuNPs are not just membrane-penetrating but actually thermodynamically prefer a membrane-embedded state, where the hydrophilic sulfonate headgroups in the amph-AuNP ligand shell 'snorkel' to the aqueous/lipid interface much like transmembrane proteins to achieve a state where free energy is minimized. 48 These studies further suggested that amph-AuNP embedding in membranes is governed by NP core size and surface ligand composition (e.g., hydrophobicity). These findings prompted us to revisit the potential for interactions of amph-AuNPs with RBCs, exploring in more detail particle localization relative to the erythrocyte membrane as a function of NP concentration and time, and the effect of membrane composition itself on NP-membrane interactions.…”

Section: Introductionmentioning

confidence: 93%

Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes

Atukorale

Bekdemir

et al. 2015

Nanoscale

Self Cite

View full text Add to dashboard Cite

Erythrocytes are attractive as potential cell-based drug carriers because of their abundance and long lifespan in vivo. Existing methods for loading drug cargos into erythrocytes include hypotonic treatments, electroporation, and covalent attachment onto the membrane, all of which require ex vivo manipulation.Here, we characterized the properties of amphiphilic gold nanoparticles (amph-AuNPs), comprised of a ∼2.3 nm gold core and an amphiphilic ligand shell, which are able to embed spontaneously within erythrocyte membranes and might provide a means to load drugs into red blood cells (RBCs) directly in vivo. Particle interaction with RBC membranes occurred rapidly at physiological temperature. We further show that amph-AuNP uptake by RBCs was limited by the glycocalyx and was particularly influenced by sialic acids on cell surface proteoglycans. Using a reductionist model membrane system with synthetic lipid vesicles, we confirmed the importance of membrane fluidity and the glycocalyx in regulating amph-AuNP/ membrane interactions. These results thus provide evidence for the interaction of amph-AuNPs with erythrocyte membranes and identify key membrane components that govern this interaction, providing a framework for the development of amph-AuNP-carrying erythrocyte 'pharmacytes' in vivo.

show abstract

Section: Introductionmentioning

confidence: 93%

Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes

Atukorale

Bekdemir

et al. 2015

Nanoscale

Self Cite

View full text Add to dashboard Cite

show abstract

“…In fact, as seen in Table 1, the size of NPs that exhibited the direct permeation is mostly less than 20 nm. Van Lehn et al (2013) showed that even with very tiny decrease in the NP size from 5.8 nm to 2.4 nm, amount of NPs entering into cells by the direct permeation significantly increased. Jiang et al (2015) also reported that the uptake amount of NPs through the direct permeation showed significant dependence on the NP size within a small range of 2 to 6 nm.…”

Section: Key Physico-chemical Properties Of Nps For Their Direct Permmentioning

confidence: 99%

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Nakamura¹,

Watano²

2018

KONA

View full text Add to dashboard Cite

Nanoparticles have attracted much attention as a key material for new biomedical and pharmaceutical applications. For success in these applications, the nanoparticles are required to translocate across the cell membrane and to reach to inside of the cell. Among several translocation pathways of nanoparticles, the direct permeation pathway has a great advantage due to its high delivery efficacy. However, despite many research efforts, key properties and factors for driving the direct permeation of nanoparticle and its underlying mechanisms are far from being understood. In this article, experimental and computational studies regarding the direct permeation of nanoparticles across a cell membrane will be reviewed. Firstly, experimental studies on the nanoparticle-cell interactions, where spontaneous direct permeation of nanoparticles was observed, are reviewed. From the experimental studies, potential key physico-chemical properties of nanoparticles for their direct permeation are discussed. Secondly, physical methods such as electroporation and sonoporation for delivering nanoparticles into cells are reviewed. Current status of technologies for facilitating the direct permeation of nanoparticle is presented. Finally, we review molecular dynamics simulation studies and present the latest findings on the underlying molecular mechanisms of the direct permeation of nanoparticle.

show abstract

“…39 Free energy calculations were also made regarding surface-capped AuNP penetration, and hydrophobic effects were highlighted. 40 These simulation efforts, however, emphasize mainly the surface ligands without addressing the role of the gold core. Most AuNPs prepared in aqueous solutions are loosely capped by citrate, yielding a moderate electrostatic protection.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Interfacing Zwitterionic Liposomes with Inorganic Nanomaterials: Surface Forces, Membrane Integrity, and Applications

Liu

2016

Langmuir

124

View full text Add to dashboard Cite

show abstract

Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers

Cited by 249 publications

References 49 publications

Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes

Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Interfacing Zwitterionic Liposomes with Inorganic Nanomaterials: Surface Forces, Membrane Integrity, and Applications

Contact Info

Product

Resources

About