Effect of penicillins on the acidification of yogurt made from ewe's milk during the storage

Berruga, M.I.; Beltrán, M.C.; Novés, B.; Molina, A.; Molina, M.P.

doi:10.1142/9789814354868_0028

Cited by 5 publications

(7 citation statements)

References 8 publications

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“…Due to interspecies differences, available bovine data cannot be accurately extrapolated for the use in the dairy ewes and goats (Pengov and Kirbis, 2009). Drug residues in milk supplies not only have public health implications (Phillips et al, 2004;Sanders et al, 2011), but may also interfere in the manufacture of dairy products, such as cheese and yogurt (Packham et al, 2001;Berruga et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Charm maximum residue limit β-lactam and tetracycline test for the detection of antibiotics in ewe and goat milk

Beltrán

Romero

Althaus

et al. 2013

Journal of Dairy Science

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The Charm maximum residue limit β-lactam and tetracycline test (Charm MRL BLTET; Charm Sciences Inc., Lawrence, MA) is an immunoreceptor assay utilizing Rapid One-Step Assay lateral flow technology that detects β-lactam or tetracycline drugs in raw commingled cow milk at or below European Union maximum residue levels (EU-MRL). The Charm MRL BLTET test procedure was recently modified (dilution in buffer and longer incubation) by the manufacturers to be used with raw ewe and goat milk. To assess the Charm MRL BLTET test for the detection of β-lactams and tetracyclines in milk of small ruminants, an evaluation study was performed at Instituto de Ciencia y Tecnologia Animal of Universitat Politècnica de València (Spain). The test specificity and detection capability (CCβ) were studied following Commission Decision 2002/657/EC. Specificity results obtained in this study were optimal for individual milk free of antimicrobials from ewes (99.2% for β-lactams and 100% for tetracyclines) and goats (97.9% for β-lactams and 100% for tetracyclines) along the entire lactation period regardless of whether the results were visually or instrumentally interpreted. Moreover, no positive results were obtained when a relatively high concentration of different substances belonging to antimicrobial families other than β-lactams and tetracyclines were present in ewe and goat milk. For both types of milk, the CCβ calculated was lower or equal to EU-MRL for amoxicillin (4 µg/kg), ampicillin (4 µg/kg), benzylpenicillin (≤ 2 µg/kg), dicloxacillin (30 µg/kg), oxacillin (30 µg/kg), cefacetrile (≤ 63 µg/kg), cefalonium (≤ 10 µg/kg), cefapirin (≤ 30 µg/kg), desacetylcefapirin (≤ 30 µg/kg), cefazolin (≤ 25 µg/kg), cefoperazone (≤ 25 µg/kg), cefquinome (20 µg/kg), ceftiofur (≤ 50 µg/kg), desfuroylceftiofur (≤ 50µg/kg), and cephalexin (≤ 50 µg/kg). However, this test could neither detect cloxacillin nor nafcillin at or below EU-MRL (CCβ >30 µg/kg). The CCβ for tetracyclines was also lower than EU-MRL for chlortetracycline (ewe milk: ≤ 50 µg/kg; goat milk: 75 µg/kg), oxytetracycline (≤ 50 µg/kg), and tetracycline (≤ 50 µg/kg). Regarding the 4-epimers of these tetracyclines only 4-epioxytetracycline was detected by the Charm MRL BLTET test below EU-MRL (ewe milk: 75 µg/kg; goat milk: ≤ 50 µg/kg). Acidiol had no effect on the performance of the test. The Charm MRL BLTET test could be used routinely with adapted test procedure for the fast screening of ewe and goat milk.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the Charm maximum residue limit β-lactam and tetracycline test for the detection of antibiotics in ewe and goat milk

Beltrán

Romero

Althaus

et al. 2013

Journal of Dairy Science

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“…Accordingly, routine screening has been established in farms and dairies in recent years to avoid having antibiotic residues at levels above legal limits reach production lines of milk products. Antibiotic residues in milk may also cause technological problems because they may inhibit fermentation processes required in the production of goat's milk products such as cheese or yogurt (7,17). Moreover, heat treatments commonly used in the dairy industry to guarantee the elimination of pathogens and to lower the bacterial count (BC) have to be considered, although they have hardly any influence on the concentration of antibiotics present in milk (20,(25)(26)(27).…”

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confidence: 99%

Validation of Receptor-Binding Assays To Detect Antibiotics in Goat's Milk

Beltrán

Borrás

Nagel

et al. 2014

Journal of Food Protection

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The suitability of different receptor-binding assays to detect antibiotics in raw goat's milk was investigated. Detection capability of most β-lactams and tetracyclines assessed applying the Betastar Combo, the SNAP Betalactam, the SNAP Tetracycline, and the Twinsensor tests was at or below maximum residue limits established by European legislation. Regarding test specificity, cross-reactions with antibiotics other than β-lactams and tetracyclines were not found, and no false-positive results were obtained for the Betastar Combo and the SNAP tests when bulk samples of goat's milk were analyzed. For the Twinsensor test, the false-positive rate was 1%. The performance of the Betastar Combo and the SNAP tests was practically unaffected by the milk quality parameters using individual samples of goat's milk collected at points throughout the entire lactation period (false-positive rate, ≤5%). However, a larger number of positive results were obtained by the Twinsensor test in this type of milk sample (>10%), especially in the last weeks of lactation. Interferences related to the use of the preservative azidiol were not observed in any case. Neither were any significant differences found in relation to the interpretation method (visual versus instrumental) applied. In general, the response of the Betastar Combo, SNAP, and Twinsensor tests was optimal for the analysis of bulk caprine milk; thus, they may be used to monitor milk for the presence of β-lactam and tetracycline residues in quality control programs.

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“…Similar approaches were carried out in more recent years to study the effect of residues of different types of antibiotics in the production of cheese [74,83,84,86,95,98,99] and yogurt [100][101][102][103]. For instance, antibiotic-free goat milk was spiked individually with seven antibiotics (amoxicillin, benzylpenicillin, cloxacillin, erythromycin, ciprofloxacin, enrofloxacin, and oxytetracycline) at an equivalent concentration of the European Union MRL and was used to make Tronchón mature cheeses [74].…”

Section: Marth and Ellicksonmentioning

confidence: 99%

Antibiotics in Dairy Production: Where Is the Problem?

Virto

Santamarina-García

Amores

et al. 2022

Dairy

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Antibiotics have long been used for the prevention and treatment of common diseases and for prophylactic purposes in dairy animals. However, in recent decades it has become a matter of concern due to the widespread belief that there has been an abuse or misuse of these drugs in animals and that this misuse has led to the presence of residues in derived foods, such as milk and dairy products. Therefore, this review aims to compile the scientific literature published to date on the presence of antibiotic residues in these products worldwide. The focus is on the reasons that lead to their presence in food, on the potential problems caused by residues in the characteristics of dairy products and in their manufacturing process, on the development and spread of antibiotic-resistant bacteria, and on the effects that both residues and resistant bacteria can cause on human and environmental health.

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Effect of penicillins on the acidification of yogurt made from ewe's milk during the storage

Cited by 5 publications

References 8 publications

Evaluation of the Charm maximum residue limit β-lactam and tetracycline test for the detection of antibiotics in ewe and goat milk

Evaluation of the Charm maximum residue limit β-lactam and tetracycline test for the detection of antibiotics in ewe and goat milk

Validation of Receptor-Binding Assays To Detect Antibiotics in Goat's Milk

Antibiotics in Dairy Production: Where Is the Problem?

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