Effect of pH, Viscosity and Ionic-Strength Changes on Ciliary Beating Frequency of Human Bronchial Explants

Luk, Chun Ka; Dulfano, Mauricio J.

doi:10.1042/cs0640449

Cited by 117 publications

(77 citation statements)

References 7 publications

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“…Diluted seawater solutions contain far lower mineral content due to dilution. Moreover non‐diluted seawater alkaline pH (7.9) is more favorable to the ciliary beat, as previously shown in vitro,51 whereas normal saline acidic pH (5.21) is deleterious for the in vitro ciliary beat 51…”

Section: Discussionmentioning

confidence: 56%

Non-diluted seawater enhances nasal ciliary beat frequency and wound repair speed compared to diluted seawater and normal saline

Bonnomet

Luczka

Coraux

et al. 2016

Int Forum Allergy Rhinol.

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BackgroundThe regulation of mucociliary clearance is a key part of the defense mechanisms developed by the airway epithelium. If a high aggregate quality of evidence shows the clinical effectiveness of nasal irrigation, there is a lack of studies showing the intrinsic role of the different irrigation solutions allowing such results. This study investigated the impact of solutions with different pH and ionic compositions, eg, normal saline, non‐diluted seawater and diluted seawater, on nasal mucosa functional parameters.MethodsFor this randomized, controlled, blinded, in vitro study, we used airway epithelial cells obtained from 13 nasal polyps explants to measure ciliary beat frequency (CBF) and epithelial wound repair speed (WRS) in response to 3 isotonic nasal irrigation solutions: (1) normal saline 0.9%; (2) non‐diluted seawater (Physiomer®); and (3) 30% diluted seawater (Stérimar). The results were compared to control (cell culture medium).ResultsNon‐diluted seawater enhanced the CBF and the WRS when compared to diluted seawater and to normal saline. When compared to the control, it significantly enhanced CBF and slightly, though nonsignificantly, improved the WRS. Interestingly, normal saline markedly reduced the number of epithelial cells and ciliated cells when compared to the control condition.ConclusionOur results suggest that the physicochemical features of the nasal wash solution is important because it determines the optimal conditions to enhance CBF and epithelial WRS thus preserving the respiratory mucosa in pathological conditions. Non‐diluted seawater obtains the best results on CBF and WRS vs normal saline showing a deleterious effect on epithelial cell function.

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Section: Discussionmentioning

confidence: 56%

Non-diluted seawater enhances nasal ciliary beat frequency and wound repair speed compared to diluted seawater and normal saline

Bonnomet

Luczka

Coraux

et al. 2016

Int Forum Allergy Rhinol.

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“…In contrast, the ciliary beating frequency of the bovine model system was little changed over the saline concentration of interest. Human bronchial ciliary beat frequency has also been reported to be unaltered in the range of 160 to 400 mosM saline (15). All the sputa had saline contents in this range, both before and after the addition of sodium chloride.…”

Section: Discussionmentioning

confidence: 86%

Sodium chloride increases the ciliary transportability of cystic fibrosis and bronchiectasis sputum on the mucus-depleted bovine trachea.

Wills

Hall²,

Chan³

et al. 1997

J. Clin. Invest.

119

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On the mucus-depleted bovine trachea, the ciliary transport rate of sputum from patients with cystic fibrosis and bronchiectasis of other causes was slow, but the rate was doubled by increasing the sodium chloride content by 90 mM. Increasing the sputum osmolality by inspissation or by the addition of nonelectrolytes had a similar effect. The viscoelasticity of sputum, but not the bovine ciliary beat frequency, was markedly saline dependent over the pathophysiological range. This suggests that low mucus salinity, not (as is generally assumed) its underhydration, contributes to its retention in bronchiectasis due to cystic fibrosis and other causes, probably by affecting its rheology. It also indicates how the genetic defect in cystic fibrosis might lead to impaired mucus clearance. Therapies that increase the osmolality of lung mucus might benefit patients with mucus retention. ( J. Clin. Invest. 1997. 99:9-13.)

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“…Baseline CBF values were then obtained by taking the mean of measurements after 1, 15 and 30 min. The pH of all solutions tested was between 7-2 and 7 4, a range known not to affect CBF (Luk & Dulfano, 1983). The extracellular Ca2+ concentration was 1-8 mm, a value within the range known not to affect the basal CBF in rabbit tracheal explants (Girard & Kennedy, 1986).…”

Section: Measurement Of Ciliary Beat Frequencymentioning

confidence: 99%

Calcium regulation of ciliary beat frequency in human respiratory epithelium in vitro.

Benedetto

Magnus

Gray

et al. 1991

The Journal of Physiology

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SUMMARY1. The changes in ciliary beat frequency (CBF) of human nasal respiratory epithelial cells were measured in vitro with a photometric technique following exposure to either 4-bromo-calcium ionophore A23187 (4-Br-A23187) or trifluoperazine (TFP), an inhibitor of calmodulin-sensitive calcium-dependent protein kinases. Changes in intracellular free calcium concentrations in response to 4-Br-A23187 were studied using a fluorescent dye (Fura-2).2. Addition of 10' M-4-Br-A23187 caused a time-dependent (P < 001) rise in CBF. The increment in CBF was statistically significant 10 min after challenge (+ 10 %; P < 0 01) and was sustained for at least 1 h, with maximal stimulation after 40 min (+ 18 %; P < 0-01).3. Exposure to 10' M-4-Br-A23187 caused an immediate increase in intracellular free calcium concentration, which preceded the rise in CBF.4. TFP (10' M) caused a reduction of baseline CBF (-10%; P < 001) and prevented the expected rise when the cells were subsequently exposed to 10' M-4-Br-A23187.5. We conclude that: (1) calcium ionophore stimulates the CBF of human respiratory cells; (2) this effect is mediated through a calmodulin-sensitive system, since it is abolished in the presence of TFP; (3) the same pathway appears to control the basal CBF of these cells, since TFP also decreases CBF.

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Effect of pH, Viscosity and Ionic-Strength Changes on Ciliary Beating Frequency of Human Bronchial Explants

Cited by 117 publications

References 7 publications

Non-diluted seawater enhances nasal ciliary beat frequency and wound repair speed compared to diluted seawater and normal saline

Non-diluted seawater enhances nasal ciliary beat frequency and wound repair speed compared to diluted seawater and normal saline

Sodium chloride increases the ciliary transportability of cystic fibrosis and bronchiectasis sputum on the mucus-depleted bovine trachea.

Calcium regulation of ciliary beat frequency in human respiratory epithelium in vitro.

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