Effect of Phosphatidic Acid on Human Breast Cancer Cells Exposed to Doxorubicin

Slíva, Daniel; Harvey, Kevin; Mason, Rebekah; Lloyd, Frank P.; English, Denis

doi:10.1081/cnv-100107739

Cited by 21 publications

(18 citation statements)

References 32 publications

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“…Cell cycle analysis was performed on a FACStarPLUS flow cytometer (Becton-Dickinson, San Jose, CA, USA) as previously described by Sliva et al (2001). Data are the mean ± SD from three independent experiments.…”

Section: Cell Cycle Analysismentioning

confidence: 99%

Immunostimulatory and anti-tumor activity of a water-soluble polysaccharide from Phellinus baumii mycelia

Xue

Sun

Zhao

et al. 2010

World J Microbiol Biotechnol

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A 17-kDa water-soluble polysaccharide (PB) was isolated and purified from Phellinus baumii using the DEAE-Sephadex A-50 and LPLC-Sephadex G-75 methods. Exposure of RAW264.7 macrophages to PB resulted in a significant increase of the cellular proliferation rate, nitric oxide production and expression levels of the IL-1b, IL-18, IL-6, IL-12p35 and IL-12p40 genes. An MTT assay indicated that PB markedly suppressed the proliferation of HepG 2 human liver cancer cells in a dose-dependent manner. Cell cycle analysis demonstrated that PB caused cell cycle arrest at the S phase, and 400 lg/ml of PB induced apoptotic cell death in HepG 2 cells after 48 h. The results suggested that PB inhibited the proliferation of HepG 2 cells by inducing cell cycle arrest at S phase, leading to apoptosis. In summary, our data indicate that the PB exerts immunoregulatory and anti-tumor activities in vitro.

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Section: Cell Cycle Analysismentioning

confidence: 99%

Immunostimulatory and anti-tumor activity of a water-soluble polysaccharide from Phellinus baumii mycelia

Xue

Sun

Zhao

et al. 2010

World J Microbiol Biotechnol

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“…After incubation, the cells were harvested by trypsinisation, washed with Dulbecco's phosphate-buffered saline containing 2% FBS, and resuspended in propidium iodine (50 mg ml À1 ). Cell cycle analysis was performed on a FACStar PLUS flow cytometer (BectonDickinson, San Jose, CA, USA), as previously described (Sliva et al, 2001). Data are the mean±s.d.…”

Section: Cell Cycle Analysismentioning

confidence: 99%

Phellinus linteus suppresses growth, angiogenesis and invasive behaviour of breast cancer cells through the inhibition of AKT signalling

et al. 2008

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The antitumour activity of a medicinal mushroom Phellinus linteus (PL), through the stimulation of immune system or the induction of apoptosis, has been recently described. However, the molecular mechanisms responsible for the inhibition of invasive behaviour of cancer cells remain to be addressed. In the present study, we demonstrate that PL inhibits proliferation (anchorage-dependent growth) as well as colony formation (anchorage-independent growth) of highly invasive human breast cancer cells. The growth inhibition of MDA-MB-231 cells is mediated by the cell cycle arrest at S phase through the upregulation of p27Kip1 expression. Phellinus linteus also suppressed invasive behaviour of MDA-MB-231 cells by the inhibition of cell adhesion, cell migration and cell invasion through the suppression of secretion of urokinase-plasminogen activator from breast cancer cells. In addition, PL markedly inhibited the early event in angiogenesis, capillary morphogenesis of the human aortic endothelial cells, through the downregulation of secretion of vascular endothelial growth factor from MDA-MB-231 cells. These effects are mediated by the inhibition of serinethreonine kinase AKT signalling, because PL suppressed phosphorylation of AKT at Thr 308 and Ser 473 in breast cancer cells. Taken together, our study suggests potential therapeutic effect of PL against invasive breast cancer.

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“…Fluorescence and phase contrast microscopy x 400. inhibition of PP1 [13] or that PA determines a range of effects associated with proliferation and cell survival (reviewed in [1]). Other studies give evidence of opposite effects, such as potentiation by PA of doxorubicin-induced apoptosis in breast cancer cells [14]. Dual effects of phosphatidic acid have also been described on smooth muscle contractility [15].…”

Section: Discussionmentioning

confidence: 98%

“…After treatment with increasing concentrations of H 2 O 2 , the percent of necrotic cells is dramatically increasing, but the percent of apoptotic cells remains low (12)(13)(14)(15)(16)(17), indicating that apoptosis is not the main mechanism of cell death [9]. Exogenous H 2 O 2 may produce necrosis by lipid peroxidation [10] and NF-B activation [11,12].…”

Section: Discussionmentioning

confidence: 99%

Selective protection by phosphatidic acid against staurosporine‐induced neuronal apoptosis

Popescu

Vidulescu

Stanciu

et al. 2002

J Cellular Molecular Medi

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Phosphatidic acid, the main product of lipid breakdown through phospholipase D activation, has been implicated in important signal transduction pathways able to influence cell fate in many ways. The purpose of this work was to determine possible effects of phosphatidic acid on neuronal cell death pathways. Here we used cerebellar granular cell cultures and cell death was triggered with either staurosporine or H2O2. Cell viability was quantified by spectrophotometry, using the 3‐ (4, 5‐dimethylthiazol‐2‐y1)‐2, 5‐diphenyl‐tetrazolium bromide (MTT) test. Staurosporine (1‐3 μM) or H2O2 (50‐800 μM) induced cell death in a dose‐dependent manner. Using fluorescent staining (propidium iodide or annexin V‐Cy3/6‐carboxyfluorescein) we showed that cell death was mostly apoptotic in staurosporine treated cells and mostly non‐apoptotic (necrotic) in H2O2 treated cells. Phosphatidic acid was able to increase cell viability in staurosporine‐, but not in H2O2 ‐ treated cells. We therefore conclude that phosphatidic acid has neuroprotective potential in neurons exposed to stimuli that trigger apoptosis.

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Effect of Phosphatidic Acid on Human Breast Cancer Cells Exposed to Doxorubicin

Cited by 21 publications

References 32 publications

Immunostimulatory and anti-tumor activity of a water-soluble polysaccharide from Phellinus baumii mycelia

Immunostimulatory and anti-tumor activity of a water-soluble polysaccharide from Phellinus baumii mycelia

Phellinus linteus suppresses growth, angiogenesis and invasive behaviour of breast cancer cells through the inhibition of AKT signalling

Selective protection by phosphatidic acid against staurosporine‐induced neuronal apoptosis

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