Effect of Plant Growth, Isolation and Purification Conditions on Barley Protoplast Yield

Donor tissue and culture condition effects on mesophyll protoplasts of Medicago sativa

“…The greater water potential may also have allowed for easier cuticle peeling, thus preventing mechanical damage to the mesophyll cells. Distilled water pretreatment was critical to protoplast viability whether conducted in light or darkness while, in previous work with other species, dark or low light pretreatments were critical to protoplast production [7,10,25] and subsequent cell division [8,24]. Stressful growth conditions could apparently be negated by pretreatment in water of excised shoots.…”

Cloning: Plants – Micropropagation/Tissue Culture

“…Dunwell and Perry (1973) found enhanced haploid plantlet production from anthers taken from stock plants grown under high photon flux, whereas Hughes et al (1978) obtained higher protoplast yield if the donor plants were grown under low light. Dunwell and Perry (1973) found enhanced haploid plantlet production from anthers taken from stock plants grown under high photon flux, whereas Hughes et al (1978) obtained higher protoplast yield if the donor plants were grown under low light.…”

Environmental and Hormonal Effects in Micropropagation

“…Concern for light intensity applied to the stock plant was given little and infrequent attention in the early years of plant tissue culture work. Dunwell and Perry (1973) found that a greater yield of haploid plantlets could be obtained from anthers taken from tobacco plants grown under high light intensities, while Hughes, et al (1978) found that a high yield of protoplasts could be produced from barley stock plants grown under low light intensities. Both of these reports were with plants grown under short-day conditions, but Heide (1968) illustrated a positive effect of long days for begonia leaf cultures and Paterson, et al (1979) showed an increased regeneration ability for explants from jade plants held in darkness on short days.…”