Effect of Platelet-Rich Plasma on CCl₄-Induced Chronic Liver Injury in Male Rats

Abstract

Platelet-rich plasma (PRP) has been of great concern to the scientists and doctors who are involved in wound healing and regenerative medicine which focuses on repairing and replacing damaged cells and tissues. Growth factors of platelet-rich plasma are cost-effective, available, and is more stable than recombinant human growth factors. Given these valuable properties, we decided to assess the effect of PRP on CCl4-induced hepatotoxicity on rats. The rats received CCl4 (1 mL/kg, i.p. 1 : 1 in olive oil) twice per week for 8 weeks. Five weeks after CCl4 injection, the rats also received PRP (0.5 mL/kg, s.c.) two days a week for three weeks. Twenty-four hours after last CCl4 injection, the animals bled and their livers dissected for biochemical and histopathological studies. Blood analysis was performed to evaluate enzyme activity. The results showed that PRP itself was not toxic for liver and could protect the liver from CCl4-induced histological damages and attenuated oxidative stress by increase in glutathione content and decrease in lipid peroxidative marker of liver tissue. The results of the present study lend support to our beliefs in hepatoprotective effects of PRP.

Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study

“…For conformation of the platelet's concentration, 80 μL of the PRP sample was counted in an automatic apparatus to verify that the platelet count was greater than 1,000,000/μL. The PRP administration: 0.5 ml PRP was dissolved in phosphate buffer saline (PBS) (PRP 1:1 PBS), then immediately aspirated with micropipette, placed in a sterile insulin syringe, and injected subcutaneously [18]. Rats were sacrificed at the end of 3rd week for all groups by decapitation under light halothane anesthesia.…”

Biochemical and Molecular Mechanisms of Platelet-Rich Plasma in Ameliorating Liver Fibrosis Induced by Dimethylnitrosurea

“…Due to the lack of effective treatment for such disorders, research is ongoing to create new medications. The liver is a hematopoietic organ during the fetal period, and fully developed hepatocytes produce thrombopoietin, which can stimulate platelet production in the bone marrow [1]. Chronic liver disease (CLD) is a disease of the liver that involves progressive destruction and regeneration of the liver parenchyma, leading to fibrosis and cirrhosis, and is the main cause of liver-related mortality and morbidity worldwide.…”

“…Interestingly, PRP has a thrombotic impact. Platelets contain proteins required for hemostasis as well as numerous growth factors [1].…”

Response to the Letter to the Editor—Re: A randomized controlled experimental study of the efficacy of platelet-rich plasma and hyaluronic acid for the prevention of adhesion formation in a rat uterine horn model

“…In addition, the same G-force applied to two different blood samples may result in different platelet concentrations, depending on the pre-centrifuge platelet concentration of the blood. In fact, our goal was a 650-700 9 10 3 lL platelet concentration, and we actually achieved a platelet concentration of 688 9 10 3 lL, similar to the cited study [2]. We followed the same RPMs for the same time periods as the authors.…”