Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L.

Paul, A.; Mitter, Kalyan; Raychaudhuri, Sarmistha Sen

doi:10.1007/s11240-009-9529-7

Cited by 43 publications

(26 citation statements)

References 47 publications

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“…Similar results were also reported by Dewi et al (2001) and Dewi and Purwoko (2008). Paul et al (2009) studied the influence of putrescine (0.1, 0.5, and 1 mM), spermidine (0.1, 0.5, and 1 µM), and spermine (0.1, 0.5, and 1 µM) on somatic embryogenesis in M. charantia L. and found that 1 mM putrescine concentration was the most effective in terms of somatic embryogenesis, followed by 0.1 µM concentration of spermine and spermidine, which also supports our findings. The results of both studies showed that spermine and spermidine should be used in much lower doses than putrescine to enhance somatic embryogenesis and plant regeneration.…”

Section: Discussionsupporting

confidence: 92%

Effect of polyamines on somatic embryogenesis via mature embryo in wheat

Aydın¹,

Türkoğlu²,

Haliloğlu³

et al. 2016

Turk J Biol

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Plant tissue culture via somatic embryogenesis plays a key role in wheat genetic transformation studies. Therefore, the production of embryogenic calli with a high regeneration capacity is a prerequisite for efficient plant regeneration. Although immature embryos are the best type of explants for plant regeneration via somatic embryogenesis, the use of mature embryos has remarkable advantages compared to immature embryos as explants. However, the regeneration capacity of mature embryos is still lower than that of immature embryos. Plant regeneration from somatic embryos can only occur when they are mature enough. Moreover, the maturation of somatic embryos and their conversion into plantlets is closely associated with the used plant growth regulators. Polyamines are major components in the formation of somatic embryogenesis and plant regeneration. In this study, the effect of three types of polyamines on somatic embryogenesis and plant regeneration in wheat was evaluated. Although the effect of polyamines on embryogenic callus formation was not significant, their effect on responded embryogenic callus rate and plant regeneration efficiency was very significant. The highest responded embryogenic callus rate and highest regeneration efficiency were obtained on MS medium containing putrescine. Unlike other polyamines, an increase in putrescine concentration promoted a higher number of regenerated plants. The highest values for responded embryogenic callus and regeneration efficiency were determined in 1 mM concentration of putrescine. Plantlets derived from somatic embryos were successfully established in soil, producing fertile seeds.

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Section: Discussionsupporting

confidence: 92%

Effect of polyamines on somatic embryogenesis via mature embryo in wheat

Aydın¹,

Türkoğlu²,

Haliloğlu³

et al. 2016

Turk J Biol

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“…The addition of PAs favored embryogenic responses in different species (Ahmadi et al, 2014;Kevers et al, 2000;Paul et al, 2009;Steiner et al, 2007;Yadav and Rajam, 1998). In Hevea brasiliensis, the addition of PAs increased the amount of embryogenic callus and the number of embryos per gram of callus using 50 µM Spd (El-Hadrami et al, 1989a, b) and also in Brassica napus the addition of 2.5 µM Put for 48 h increased three-fold the embryo formation and 5.56 µM Put increased fourfold the embryo conversion (Ahmadi et al, 2014).…”

Section: Effect Of Polyamines On the Efficiency And Developmental Mormentioning

confidence: 99%

Polyamines and WOX genes in the recalcitrance to plant conversion of somatic embryos of Habanero pepper (Capsicum chinense Jacq.)

Valle-Gough¹,

Avilés-Viñas²,

López-Erosa³

et al. 2015

Afr. J. Biotechnol.

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In order to determine the role of polyamines in the formation and development of the somatic embryos of Capsicum chinense, the effect of different concentrations (0, 0.01, 0.1, and 1.0 mM) of Putrescine, Spermidine and Spermine on the efficiency and morphology of the embryos was evaluated. The results show that none of the three polyamines evaluated had a significant effect on the number of embryos formed, except Spermidine (1 mM), which caused a significant reduction in their numbers, in comparison with the control treatment. However, the most noteworthy result was observed in the treatment containing 0.1 mM of Spermine. The embryos developed in this treatment showed harmonic apex-radicle development, pale-green coloration and the formation of two tiny cotyledonary leaves. Real-time PCR was used to analyze the differential expression of the WUS, WOX1 and WOX3 genes in somatic embryos treated with Spermine and untreated, including the zygotic embryo. The transcript levels of the genes analyzed were found to differ significantly between both types of embryos (somatic and zygotic), with the zygotic embryos presenting a higher level of transcripts; however, compared to the untreated somatic embryos, the somatic embryos treated with Spermine showed an increase in the transcript levels of the three genes analyzed (WUS, WOX1 and WOX3); the WOX1 gene in particular presented an accumulation pattern similar to that of the zygotic embryo of the species.

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“…In another report, Paul et al ( 2009 ) investigated the effect of various polyamines (PA) on somatic embryo induction in M. charantia . Somatic embryos were induced from leaf explants on MS medium supplemented with 2.7 μM NAA and 22.0 μM BA after 21 days of culture.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

In Vitro Strategies for the Conservation of Some Medicinal and Horticultural Climbers

Thomas

Hoshino

2015

Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers

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There has been an increase in use of traditional medicine during the last decade due to the reason that medicinal plant-derived drugs are more effective against several diseases as compared to antibiotics and other medicines and the former has less side effects on patients. Moreover, medicinal plants are a rich source of antibacterial compounds. The increased use and harvest of medicinal plants coupled with habitat destruction caused a decrease in natural population of most of the medicinal plants. In vitro conservation strategies can not only offer large number of plants in limited time but also it can decrease tension on natural populations. Similarly, several horticultural plants including vegetable crops need improvements and therefore in vitro conservation strategies could be applied for their further progress. The present review is an attempt to provide information on various in vitro conservation strategies adopted in selected medicinal and horticultural climbers. The important medicinal and horticultural climbers such as Benincasa hispida ,

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Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L.

Cited by 43 publications

References 47 publications

Effect of polyamines on somatic embryogenesis via mature embryo in wheat

Effect of polyamines on somatic embryogenesis via mature embryo in wheat

Polyamines and WOX genes in the recalcitrance to plant conversion of somatic embryos of Habanero pepper (Capsicum chinense Jacq.)

In Vitro Strategies for the Conservation of Some Medicinal and Horticultural Climbers

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