Effect of polycaprolactone on physicochemical, biological, and mechanical properties of polyethylene oxide and polyamino acids nano block copolymers

Abstract

This work aims to design robust and biocompatible nano blockcopolymers (NBCPs) of polycaprolactone@-polyethylene oxide-polyaminoacid (PCL@-PEO-PAAs) by ring-opening polymerization. Mechanical behavior including modulus, elongation at yield and break of the tetra block copolymer are studied at room temperature. The significant result lies when the extension

Morphology biased pharmacological and mechanical properties of nanosized block copolymers of PNIPAM with polyethylene oxide and polyaminoacids in presence of polycaprolactone

“…It is also studied that the spherical necklace rings are interconnected to each other by several chemical bonding which depends on external parameters such as sonication time, solvent, staining chemicals, and so on. 27 Figure 3a-d and the insets of Figure 3c, d represent very interesting morphology for PEO-b-PAAs-b-PNI-PAM@PCL NBCPs. This polymer looks like a petunia bell-shaped flower-like structure at lower magnification.…”

“…The reason can be explained by the involvement of thermally stable PCL in the latter case. 27 However, a shifted onset of degradation to higher temperatures and an additional bump is observed for PEO-b-PAAs-b-PNIPAM NBCPs due to the absence of PCL in it. It is also observed that former NBCPs lost 76% of their mass while the latter NBCPs lost 84% at 450 C. hence these NBCPs can be utilized up to 340 C for biomedical applications or tissue engineering purposes.…”

“…All the reagents and solvents are taken for the design of NBCPs without any further purification which is mentioned below: CDCl 3 (>99.9%, FINAR), acetone (>96%, FINAR), ethanol (>97.9%, FINAR), THF (>96%, FINAR), MTT (methyl thiazolyl tetrazolium, 99.9%, Sigma-Aldrich), PNIPAM (>98%, Sigma-Aldrich), polycaprolactone (Mw 70-90 k, Sigma-Aldrich), L-aspartic acid-β-benzyl ester (>99%, Sigma-Aldrich), L-glutamic acid-γ-benzyl ester (>99%, Sigma-Aldrich), polyethylene oxide (>99%, Sigma-Aldrich), 10% activated Pd/C catalyst (>99.9%, Sigma-Aldrich), phosphotungstic acid (PTA) (>93.8%, Sigma-Aldrich), triphosgene (>99.9%, Sigma-Aldrich), chloroquine diphosphate salt (98.5%-101%, Sigma Aldrich), ciprofloxacin (≥98%, HPLC Sigma Aldrich), ofloxacin (Sigma Aldrich), ornidazole (Sigma Aldrich). ] or PEO-PAAs TBP via ROP technique by following our previous report 27,28 followed by purification methods, such as evaporation, crystallization, recrystallization and drying process. In the subsequent step, we have taken a mixture of 1 eq.…”

“…The results obtained from the MTT assay indicated that both the NBCPs seem to avail a good cell survival percentage of >80% up to 200 μg/ml against the cancer cells. 27 It is revealed that the synthesized polymeric solutions exhibit low cytotoxicity against both cancer cells proving the designed NBCPs as better targeted drug carriers to resolve cancer infections. The increment of such performances under in vivo conditions is mainly caused by the branched structure which inhibits them to go out from the infected cell lines.…”

“…There are many reports available with MTT assay of polymer up to 200 μg/ml 46 but a few reports associated with MTT study >200 μg/ml. 27 In the present study, we intended to study its behavior up to 1000 μg/ml condition. The results obtained from the MTT assay indicated that both the NBCPs seem to avail a good cell survival percentage of >80% up to 200 μg/ml against the cancer cells.…”