Effect of Polyelectrolyte Adsorption on Intercolloidal Forces

Borukhov, Itamar; Andelman, David; Orland, Henri

doi:10.1021/jp990055r

Cited by 106 publications

(175 citation statements)

References 38 publications

Supporting

Mentioning

169

Contrasting

Order By: Relevance

“…Repeating the adsorption of alternating positively and negatively charged PE's, it is possible to create a multilayer structure of PE's at the surface. Although many experiments and potential applications for PE multilayers exist, the theory of PE overcompensation is only starting to be developed [157,190,191,193,205,206,209]. The scaling laws presented for constant U s can be used also for the case of constant surface charge.…”

Section: Adsorption Of Pe's For Constant Surface Charge and Its Overcmentioning

confidence: 99%

Neutral and charged polymers at interfaces

2003

Self Cite

View full text Add to dashboard Cite

Chain-like macromolecules (polymers) show characteristic adsorption properties due to their flexibility and internal degrees of freedom, when attracted to surfaces and interfaces. In this review we discuss concepts and features that are relevant to the adsorption of neutral and charged polymers at equilibrium, including the type of polymer/surface interaction, the solvent quality, the characteristics of the surface, and the polymer structure. We pay special attention to the case of charged polymers (polyelectrolytes) that have a special importance due to their water solubility. We present a summary of recent progress in this rapidly evolving field. Because many experimental studies are performed with rather stiff biopolymers, we discuss in detail the case of semi-flexible polymers in addition to flexible ones. We first review the behavior of neutral and charged chains in solution. Then, the adsorption of a single polymer chain is considered. Next, the adsorption and depletion processes in the many-chain case are reviewed. Profiles, changes in the surface tension and polymer surface excess are presented. Mean-field and corrections due to fluctuations and lateral correlations are discussed. The force of interaction between two adsorbed layers, which is important in understanding colloidal stability, is characterized. The behavior of grafted polymers is also reviewed, both for neutral and charged polymer brushes.

show abstract

Section: Adsorption Of Pe's For Constant Surface Charge and Its Overcmentioning

confidence: 99%

Neutral and charged polymers at interfaces

2003

Self Cite

View full text Add to dashboard Cite

show abstract

“…Obtaining attractive interactions between likecharged macroions is also possible on the level of PB theory if additional order parameters are considered. This is the case, for instance, upon including the solvent structure [12] or the presence of polyelectrolytes [13,14] into PB theory.…”

Section: Edp Sciencesmentioning

confidence: 99%

Interaction between macroions mediated by divalent rod-like ions

2004

View full text Add to dashboard Cite

Abstract. -Attractive interactions between identical like-charged macroions in aqueous multivalent salt solution arise due to ion-ion correlations. The mean-field level Poisson-Boltzmann (PB) theory does not predict such behavior for point-like structureless ions. Various multivalent ions, such as certain DNA condensing agents or short stiff polyelectrolytes, do have an internal, often rod-like, structure. Applying PB theory to the generic case of divalent rod-like salt ions, we find attraction between like-charged macroions above a critical distance between the two individual charges of the rod-like ions. We calculate this distance analytically within linearized PB theory. Numerical results for the non-linear PB theory indicate strong enhancement of the tendency to mediate attractive interactions.Introduction. -Poisson-Boltzmann (PB) theory is a widely used mean-field level method to calculate interactions between macroions in aqueous (salty) solutions. For monovalent salt, its predictions are generally found to agree well with experimental results and computer simulations. However, the presence of multivalent ions can affect the nature of the interactions between macroions in a way that qualitatively differs from the PB prediction. A remarkable example is the possibility of attraction between two identical, like-charged, macroions that the mean-field approach is unable to predict. This attraction currently receives much interest [1] because it is observed for a number of biologically relevant processes such as condensation of DNA [2], network formation in actin solutions [3], virus aggregation [4] and interactions between lipid membranes that occur during adhesion and fusion. Various theoretical approaches ascribe this attraction to the presence of ion-ion correlations [5,6]. An intuitive understanding of these correlations can be based on the formation of a periodic counterion arrangement in the vicinity of each macroion, similar to a Wigner crystal. The two inter-locked counteriondecorated macroions then experience short-ranged attraction.

show abstract

“…The organization of F-actin is controlled predominantly by actin binding proteins (ABP's), which crosslink actin into a polymorphism of bundle and network phases [1][2][3], through a process that has recently been examined theoretically [4,5]. Espins are a recently discovered class of ABP's responsible for the formation of parallel actin bundles in vivo and in vitro [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Structural Polymorphism of the Actin-Espin System: A Prototypical System of Filaments and Linkers in Stereocilia

2007

View full text Add to dashboard Cite

We examine the interaction between cytoskeletal F-actin and espin 3A, a prototypical actin bundling protein found in sensory cell microvilli, including cochlear cell stereocilia. Espin induces twist distortions in F-actin as well as facilitates bundle formation. Mutations in one of the two F-actin binding sites of espin, which have been implicated in deafness, can tune espin-actin inter-actions and radically transform the system's phase behavior. These results are compared to recent theoretical work on the general phase behavior linker-rod systems.Filamentous actin (F-actin), a biological rod-like polyelectrolyte, is a principal component of the eukaryotic cytoskeleton. The organization of F-actin is controlled predominantly by actin binding proteins (ABP's), which crosslink actin into a polymorphism of bundle and network phases [1][2][3], through a process that has recently been examined theoretically [4,5]. Espins are a recently discovered class of ABP's responsible for the formation of parallel actin bundles in vivo and in vitro [6][7][8]. A specific isoform, espin 3A, is found in actin bundles in sensory cell microvilli, such as the stereocilia of cochlear hair cells, which are vital for the transduction of sound in hearing [9][10][11]. Genetic mutations in espin's F-actin binding sites are linked to malformed stereocilia, deafness and vestibular dysfunction in humans and mice [11][12][13]. Such 'deafness' mutations allow us to assess the role of linker 'stickiness' in the organization of actin bundles.In this Letter we describe the first measurements of self-assembled espin-actin bundle structure, and examine how interactions between F-actin and different espin linkers are expressed in the system's phase behavior. Small angle x-ray scattering (SAXS) shows that as espin concentration increases, the system evolves directly from an isotropic phase to hexagonally coordinated paracrystalline bundles of hyper-twisted filaments. As the ability of a given espin to crosslink F-actin is decreased by using two different genetically modified 'deafness' mutants of espin with progressively more severe damage to one of the two actin binding sites, an unanticipated nematic actin-espin network is observed. By mixing wild type (wt) and mutant espin, which allows us to continuously tune this crosslinking affinity between espin and actin (as well as mimic heterozygous expression of mutant espins), we find that the onset of this nematic-network phase is a strong function of espin crosslinking affinity and concentration. We hypothesize that this unexpected isotropic to nematic network transition, which has strong implications for stereocilia formation and hearing, is due to the way weak espin linkers crosslink actin rods. F-actin was prepared from lyophilized rabbit skeletal muscle G-actin monomer (42 kDa, Cytoskeleton, Inc). G-actin was resuspended in 5 mM Tris, with 0.2 mM CaCl 2 , 0.5 mM ATP, 0.2 mM Dithotreithol (DTT) and 0.01% NaN 3 at pH 8.0 and polymerized into F-actin upon the addition of 100 mM KCl. F-actin is t...

show abstract

Effect of Polyelectrolyte Adsorption on Intercolloidal Forces

Cited by 106 publications

References 38 publications

Neutral and charged polymers at interfaces

Neutral and charged polymers at interfaces

Interaction between macroions mediated by divalent rod-like ions

Structural Polymorphism of the Actin-Espin System: A Prototypical System of Filaments and Linkers in Stereocilia

Contact Info

Product

Resources

About