Effect of Polyethylene on Osteocalcin, Alkaline Phosphatase and Procollagen Secretion by Human Osteoblastic Cells

Martı́nez, Marı́a Eugenia; Medina, Sonia; Campo, Ma Teresa del; Sánchez-Cabezudo, M.; Sánchez, Miguel Ángel García; Munuera, L.

doi:10.1007/s002239900459

Cited by 13 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10C and D). Nevertheless, only a fraction of cells (approximately 40%) seeded on the polymer remained on the polymer, while the remaining of the cells migrated to the polystyrene surface in the plate [11,24]. Since the cells that migrated to the TCPS from the polymer remained healthy and indicates that the polymer and its degradation products were not significantly toxic in vitro.…”

Section: Resultsmentioning

confidence: 99%

“…Collagen type I is hydroxyproline-rich protein secreted in significant concentrations by BMSC cells [23,24]. Since collagen type I contains 10-15% hydroxyproline and it is the major protein synthesized by BMSC, we measured hydroxyproline contents in the culture supernatents to compare collagen synthesis in BMSC cultured on LDI-glycerol polymer or on the TCPS.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A new peptide-based urethane polymer: synthesis, biodegradation, and potential to support cell growth in vitro

et al. 2000

View full text Add to dashboard Cite

A novel non-toxic biodegradable lysine-di-isocyanate (LDI)-based urethane polymer was developed for use in tissue engineering applications. This matrix was synthesized with highly purified LDI made from the lysine diethylester. The ethyl ester of LDI was polymerized with glycerol to form a prepolymer. LDI-glycerol prepolymer when reacted with water foamed with the liberation of CO 2 to provide a pliable spongy urethane polymer. The LDI-glycerol matrix degraded in aqueous solutions at 100, 37, 22, and 4°C at a rate of 27.7, 1.8, 0.8, and 0.1 mM per 10 days, respectively. Its thermal stability in water allowed its sterilization by autoclaving. The degradation of the LDI-glycerol polymer yielded lysine, ethanol, and glycerol as breakdown products. The degradation products of LDI-glycerol polymer did not significantly affect the pH of the solution. The glass transition temperature (T g ) of this polymer was found to be 103.4°C. The physical properties of the polymer network were found to be adequate to support the cell growth in vitro, as evidenced by the fact that rabbit bone marrow stromal cells (BMSC) attached to the polymer matrix and remained viable on its surface. Culture of BMSC on LDI-glycerol matrix for long durations resulted in the formation of multilayered confluent cultures, a characteristic typical of bone cells. Furthermore, cells grown on LDI-glycerol matrix did not differ phenotypically from the cells grown on the tissue culture polystyrene plates as assessed by the cell growth, and expression of mRNA for collagen type I, and transforming growth factor-β1 (TGF-β1). The observations suggest that biodegradable peptide-based urethane polymers can be synthesized which may pave their way for possible use in tissue engineering applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A new peptide-based urethane polymer: synthesis, biodegradation, and potential to support cell growth in vitro

et al. 2000

View full text Add to dashboard Cite

show abstract

“…BMSCs produce copious amounts of hydroxyproline-rich type I procollagen. 25,26 To compare the synthetic characteristics of BMSCs grown on the polymer with those of BMSCs cultured on tissue culture polystyrene (TCPS), we determined the hydroxyproline content of the culture medium after a 10-day period of cell growth. As shown in Fig.…”

Section: Cell Growth On Ldi-glucose Matrix In Vitromentioning

confidence: 99%

Synthesis, Biodegradability, and Biocompatibility of Lysine Diisocyanate–Glucose Polymers

et al. 2002

View full text Add to dashboard Cite

The success of a tissue-engineering application depends on the use of suitable biomaterials that degrade in a timely manner and induce the least immunogenicity in the host. With this purpose in mind, we have attempted to synthesize a novel nontoxic biodegradable lysine diisocyanate (LDI)-and glucose-based polymer via polymerization of highly purified LDI with glucose and its subsequent hydration to form a spongy matrix. The LDI-glucose polymer was degradable in aqueous solutions at 37, 22, and 4°C, and yielded lysine and glucose as breakdown products. The degradation products of the LDI-glucose polymer did not significantly affect the pH of the solution. The physical properties of the polymer were found to be adequate for supporting cell growth in vitro, as evidenced by the fact that rabbit bone marrow stromal cells (BMSCs) attached to the polymer matrix, remained viable on its surface, and formed multilayered confluent cultures with retention of their phenotype over a period of 2 to 4 weeks. These observations suggest that the LDI-glucose polymer and its degradation products were nontoxic in vitro. Further examination in vivo over 8 weeks revealed that subcutaneous implantation of hydrated matrix degraded in vivo three times faster than in vitro. The implanted polymer was not immunogenic and did not induce antibody responses in the host. Histological analysis of the implanted polymer showed that LDIglucose polymer induced a minimal foreign body reaction, with formation of a capsule around the degrading polymer. The results suggest that biodegradable peptide-based polymers can be synthesized, and may potentially find their way into biomedical applications because of their biodegradability and biocompatibility.

show abstract

“…16,31 The smaller the polyethylene particles, the more they inhibited growth in these cells. 16 The other study demonstrated that the polyethylene particles directly affected osteoblastic markers in human bone cells in culture -they increased the secreted markers related to mineralization and chemotaxis and decreased PICP secretion.…”

Section: Discussionmentioning

confidence: 99%

“…Previous papers from our group, as well as other authors, have analyzed the in vitro effects of particulate debris on some markers of osteoblastic phenotype and found changes related to reduced osteoblastic activity with or without increased bone resorption. [26][27][28][29][30][31] These findings implicate altered osteoblast function in the bone loss associated with prosthetic loosening, therefore, a comprehensive model of this process must account for osteoclast bone resorption, as well as the possibility that osteoblast function could also be affected.…”

Section: Introductionmentioning

confidence: 99%

Effects of MA 956 superalloy and ?-alumina particles on some markers of human osteoblastic cells in primary culture

Rodrigo

Martı́nez

Martínez

et al. 2000

J. Biomed. Mater. Res.

Self Cite

View full text Add to dashboard Cite

One of the problems associated with the modern biomaterials used in prostheses is osteolysis, which, although its exact origin is unknown, has been associated with wear particles. Osteoblasts seem to participate directly in this phenomenon. This paper investigates in vitro cellular response to the wear particles from the metal substrate and ceramic covering (alpha-alumina) of a new titanium yttrium aluminum alloy, MA 956, that has been proposed as a biomaterial because of its exceptional mechanical and electrochemical properties. The effect of different sizes (10 and 80 microm) of MA 956 and alpha-alumina particles on osteoblast function was studied in primary human bone cell cultures. Cells were harvested from trabecular bone fragments obtained during knee arthroplasty. Osteoblastic cell response to the particles was measured by assaying C-terminal type I procollagen (PICP), alkaline phosphatase, and osteocalcin secretion, with and without 1.25(OH)(2)D(3) stimulation, in the cell-conditioned medium. Both sizes of MA 956 and alpha-alumina particles decreased PICP secretion in nonstimulated osteoblastic cells, but this secretion was not affected in the cultures stimulated with 1.25(OH)(2)D(3). Only the 10 microm alpha-alumina particles inhibited alkaline phosphatase activity in 1.25(OH)(2)D(3)-stimulated and nonstimulated cultures. The rise in osteocalcin levels after 1.25(OH)(2)D(3) stimulation was lower in the presence of the 10 microm MA 956 particles than in the presence of alpha-alumina particles. Although both materials seem to have directly affected in vitro osteoblastic cell function, the increase in osteocalcin levels after 1.25(OH)(2)D(3) stimulation was lower after exposure to MA 956 particles than the increase observed after exposure to alpha-alumina particles. Therefore, it does not seem that osteocalcin stimulated bone resorption, suggesting that MA 956 would be less likely to provoke osteolysis.

show abstract

Effect of Polyethylene on Osteocalcin, Alkaline Phosphatase and Procollagen Secretion by Human Osteoblastic Cells

Cited by 13 publications

References 19 publications

A new peptide-based urethane polymer: synthesis, biodegradation, and potential to support cell growth in vitro

A new peptide-based urethane polymer: synthesis, biodegradation, and potential to support cell growth in vitro

Synthesis, Biodegradability, and Biocompatibility of Lysine Diisocyanate–Glucose Polymers

Effects of MA 956 superalloy and ?-alumina particles on some markers of human osteoblastic cells in primary culture

Contact Info

Product

Resources

About