Effect of postthaw storage time and sperm-to-egg ratio on fertility of cryopreserved brook trout sperm

Nynca, Joanna; Dietrich, G.J.; Dobosz, Stefan; Zalewski, Tomasz; Ciereszko, Andrzej

doi:10.1016/j.theriogenology.2014.09.009

Cited by 38 publications

(20 citation statements)

References 12 publications

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“…To our knowledge, this is the first report regarding the verification of the effect of post-thaw storage on the sperm motility and fertilization ability of Siberian sturgeon semen. Recently Nynca et al [23] in their study have demonstrated a similar phenomenon for salmonid fish. Since a similar extender was used it can be suggested that glucose-methanol extender is useful for the post-thaw storage of semen.…”

Section: Discussionsupporting

confidence: 53%

New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen

et al. 2015

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Section: Discussionsupporting

confidence: 53%

New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen

et al. 2015

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“…For salmonids, fertility rates similar to those obtained with control sperm were obtained with sperm to egg ratios as low as 50,000:1 for grayling (Horváth et al, 2015), 300,000:1 for brook and brown trout (Nynca et al, 2014, 2015b), 500,000:1 for huchen (Nynca et al, 2015c) and 600,000:1 for rainbow trout (Ciereszko et al, 2014). Vitrification is a rarely used technique in fish sperm but with promising applications.…”

Section: Fish Sperm Cryopreservationsupporting

confidence: 59%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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“…; Nynca et al. ). Our results indicated that storage had a negative impact on the fertilizing ability of semen after 30 min.…”

Section: Discussionmentioning

confidence: 97%

“…Although high post-thaw sperm motility and fertilization ability have been achieved in some fish species due to considerable progress in optimizing the cryoprotectant, dilution ratio, freezing rate and thaw temperature for cryopreservation, the motility and fertility levels of cryopreserved sperm were still lower those of fresh samples (Frankel et al 2013;Ciereszko et al 2014). It had been assumed that thawed semen must be used immediately for fertilization and that 30 s sperm storage following thawing significantly reduces the fertilization rate (Lahnsteiner et al 1996;Nynca et al 2014). Our results indicated that storage had a negative impact on the fertilizing ability of semen after 30 min.…”

Section: Discussionmentioning

confidence: 99%

Retracted: Effect of Post‐Thaw Storage Time on Motility and Fertility of Cryopreserved Beluga Sturgeon (Huso huso) Sperm

Aramli

Nazari²,

Gharibi

2015

Reprod Domestic Animals

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The aim of this study was to test the influence of post-thaw storage time on the duration of sperm motility, percentage of motile sperm, and fertilization and hatching rates of fresh sperm and sperm stored for 0, 30 and 60 min at 4°C post-thawing. After being frozen in liquid nitrogen and then thawed, the percentage of motile sperm and duration of motility were not affected by 30 min of storage at 4°C, whereas a significant decline in these parameters was observed after 60 min of storage. Similarly, fertilization and hatching rates were significantly affected within 60 min of storage at 4°C, and the fertility of frozen-thawed sperm was significantly lower than that of fresh sperm. We conclude that cryopreserved sperm of beluga sturgeon could be stored for 30 min without the loss of sperm quality. This described procedure for beluga sturgeon cryopreservation is reliable and efficient and therefore can be recommended for hatchery practice after scaling up this technique.

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Effect of postthaw storage time and sperm-to-egg ratio on fertility of cryopreserved brook trout sperm

Cited by 38 publications

References 12 publications

New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen

New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen

Cryobanking of aquatic species

Retracted: Effect of Post‐Thaw Storage Time on Motility and Fertility of Cryopreserved Beluga Sturgeon (Huso huso) Sperm

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