Effect of preservation time on the quality of frozen semen in indigenous rams

Mahmuda, Bba; nesa, Azizun; Zohara, BF; Alam, Md. Monjurul; Bari, Farida Yeasmin

doi:10.3329/bjas.v44i1.23113

Cited by 6 publications

(5 citation statements)

References 8 publications

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“…The post-thaw sperm motility (41.3±9.5%) and viability (58.3±8.7%) were significantly (P<0.05) decreased compared with the motility and viability of fresh and post-diluted semen. This result is similar with the observation of Mahmuda et al (2015). However, the rate of motility and viability of post thawed semen in the present study were lower than observed by Azizunnesa et al (2014).…”

Section: Discussionsupporting

confidence: 92%

“…The volume, colour, mass activity, sperm motility, viability, concentration, HOST +ve (%) and normal spermatozoa (%) varied from 0.4-1.3 ml, 2-4 (creamy to creamy-grainy), 2-5, 75-92%, 80-95%, 2500-5000×10 6 /ml, 7590% and 75-95%, respectively. Similar semen quality parameters were observed in previous study of the same laboratory (Mahmuda et al, 2015 andAzizunnesa et al, 2014). In the present study the sperm concentration of ram R#6 was significantly higher (4120.5±93.5x 10 6 /ml) compared with ram R#1, R#4 and R#5.…”

Section: Discussionsupporting

confidence: 91%

“…This could be due to climatic condition, weather and season of the study. The mean motility of present study supported result observed with the progress of preservation time after freezing (Mahmuda et al, 2015). The success is the post thaw motility was not significantly lowered with the progress of preservation time, indicating the suitable protocol of freezing process in the present study.…”

Section: Discussionsupporting

confidence: 85%

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Conception rate following intra-cervical artificial insemination using frozen semen at field level in indigenous sheep of Bangladesh

Mansur

Alam

Jha

et al. 2018

Asian J. Med. Biol. Res

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This study was undertaken to study the AI conception rate using frozen semen at field level. Five farms in Mymensingh, Bangladesh were selected for AI Trial in field ewes. Four rams were selected for semen collection, evaluation, and frozen semen production and further to study conception rate followed by intracervical AI in both natural and synchronized ewes. Conception rate were confirmed by non-returned rate and ultrasound scanning at 30-40 days of post insemination. The volume, colour, mass activity, sperm motility, viability, concentration, HOST +ve (%) and normal spermatozoa percentages were 0.8±0.3 ml, 3.9±0.3, 4.4±0.6, 81.3±5.0%, 90.0±4.0%, 3519.0±545.6x10 6 /ml, 87.4±3.3% and 85.6±1.8%, respectively. The sperm concentration of ram R#6 was significantly higher (P<0.05) (4120.5±93.5x10 6 /ml) compared with other rams. The mean motility and viability of pre-dilution, 120 minutes of addition of Part-A, 240 minutes of addition of Part-B and post-thaw were (83.8±4.8%, 81.3±2.5%, 80.0±4.1% and 41.3±9.5%) and (93.3±1.0%, 90.0±1.4%, 88.8±1.0% and 58.3±8.7%), respectively. There were no significant difference (P˃0.05) between pre-dilution and post-dilution sperm motility and viability percentage however, post-thaw sperm motility and viability significantly (P<0.05) decreased compare with the motility and viability of pre-dilution and post-dilution values. Motility and viability percentages of frozen semen did not decrease significantly (P> 0.05) with the increase of preservation time. The mean motility and viability at 24 hrs, day 7, day 15 and day 30 were 41.3±9. 5%, 41.5±8.5%, 41.8±9.9% and 40.5±10.2%; and 59.0±10.1%, 58.5±7.7%, 59.0±8.8% and 57.8±8.3%, respectively. The conception rates in natural and synchronized estrous were 26.7% and 25%, respectively. There was no significant difference in conception rates between the natural and synchronized oestrous in field level. However, the present non-return rate and conception rate indicate the suitability of produced frozen semen application in the field level.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 85%

See 1 more Smart Citation

Conception rate following intra-cervical artificial insemination using frozen semen at field level in indigenous sheep of Bangladesh

Mansur

Alam

Jha

et al. 2018

Asian J. Med. Biol. Res

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“…Usually, during freezing-thawing process of semen, the sperm undergoes several vital biological and functional changes due to cold shocks and cryo-injuries, especially affects membranes that distresses their functional abilities (Nur et al, 2010;Chelucci et al, 2015). To establish a simple and sustainable cryopreservation protocol for ram semen in Bangladesh, efforts are focused on different types of diluents, dilution and freezing methods (Mahmuda et al, 2015;Rekha et al, 2016;Rekha et al, 2018;Jha et al, 2019). Evidence from previous studies, a hand-made tris-fructose-citrate (20% egg yolk and 5-7% glycerol) and commercial diluents Triladyl ® , two-steps dilution (fractional procedure) and three-steps manual freezing (in LN2 vapourusing styrofoam) are seemed to be recommended protocol for freezing ram semen in Bangladesh, already extended for field fertility testing (Rekha et al 2016;Rekha et al 2018;Jha et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

A Comparison of Freezing Methods and Diluents Types on Post-Thaw Sperm Quality of Ram Sperm

Jha¹,

Alam²,

Bari³

2020

Res. Agric. Livest. Fish.

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The effect of freezing methods and diluents types on post-thaw sperm quality of Bangladeshi ram semen was studied. Two freezing methods and three diluents was tested as pooling effects (freezing methods or diluents) on post-thaw sperm parameters; sperm motility (SM), viability (SV), plasma membrane integrity (SPMI) and acrosome integrity (SAI), respectively. From selected ten rams, eight ejaculates were used for each freezing group (freezing methods × diluents). Semen samples were diluted by using two-steps for hand-made tris-based diluents (20% egg yolk): D1 (7% glycerol) and D2 (5% glycerol), and one-step dilution for commercial diluents: D3 (Triladyl®) at 35°C. After 4h of equilibration of temperature at 5°C, diluted semen samples was aspirated into 0.25 mL straws, and sealed. Straws were frozen in liquid nitrogen (LN2) vapour using two methods: F1 (manually in Styrofoam box, using three-steps method; +5°C to -80°C at -11.33°C/min, -80°C to -120°C at -26.66°C/min, and -120°C to -140°C at - 13.33°C/min) and F2 (programmable bio-freezer, using two-steps method; +5°C to -100°C at - 20°C/min and -100°C to -140°C at -10°C/min). Two semen straws from each batch were evaluated (37°C for 20 sec) for sperm parameters. In pool effects between freezing methods; SAI differed significantly (P < 0.001). The SM (56%) and SV (72%) were observed competitive. However, SPMI (67.58 ± 2.02%) and SAI (76.13 ± 1.42%) were higher in F1. Among diluents, SM (P < 0.006), SV (P < 0.008), SPMI (P < 0.012) and SAI (P < 0.019) differed significantly. The SM (61.25 ± 1.80%), SV (77.13 ± 1.47%), SPMI (68.31 ± 1.91%) and SAI (74.75 ± 1.64%) were highest in D3. In conclusion, the combination of manual freezing (three-steps) and handmade tris-based diluents (20% egg yolk, 5% glycerol) is suitable and sustainable method for cryopreservation of ram semen. Res. Agric., Livest. Fish.7(2): 235-241, August 2020

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“…Semen cryopreservation is a crucial technique for improving assisted reproductive technologies (ART), especially AI protocols (11). While cryopreservation of ovine spermatozoa can significantly increase the time needed for storage, it also allows and assists their transport over long distances (12), thereby resolving the aforementioned problems.…”

mentioning

confidence: 99%

Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram

Bintara¹,

Maharani²,

Tavares³

et al. 2024

J Anim Sci Technol

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The primary cause of sperm quality decline during the freeze-thaw pathway is the peroxidation hazard caused by reactive oxygen species produced by the biological molecules of sperm. Ascorbic acid (Vitamin C) and lycopene are two potent antioxidants that operate to prevent oxidation processes. This study aimed to analyse the effects of ascorbic acid and lycopene on the motility, viability, abnormality and plasma membrane integrity of post-thawed Sapudi rams. Sperm samples were obtained and pooled from six sexually mature Sapudi rams, separated into ten equal proportions and diluted with TEY extender. Semen was supplemented with 0 (C0; L0), 1 (C1; L1), 2 (C2; L2), 3 (C3; L3) and 4 (C4; L4) mg/100mL (1-4%) diluent each of ascorbic acid and lycopene, respectively. Total sperm motility, viability, abnormalities and semen membrane plasma (%) were analysed after thawing. C3 and L3 extenders resulted in higher total motility (p<0.05) compared to the other extenders, with all treatments higher than that of the control. The extender C3 (p<0.05) exhibited the highest semen quality.Finally, the current findings show that C3 and L3 can increase the quality of post-thawed Sapudi ram spermatozoa.

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Effect of preservation time on the quality of frozen semen in indigenous rams

Abstract: The study was carried out to observe the effects of preservation time on the quality of frozen semen of indigenous rams. Semen was collected using AV once a week from 4 rams. Tris based with 10% egg yolk and 7% glycerol extender was used to extend and freezing the semen.

Cited by 6 publications

References 8 publications

Conception rate following intra-cervical artificial insemination using frozen semen at field level in indigenous sheep of Bangladesh

Conception rate following intra-cervical artificial insemination using frozen semen at field level in indigenous sheep of Bangladesh

A Comparison of Freezing Methods and Diluents Types on Post-Thaw Sperm Quality of Ram Sperm

Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram

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