Effect of process parameters and surfactant additives on the obtained activity of recombinant tryptophan hydroxylase (TPH1) for enzymatic synthesis of 5-hydroxytryptophan (5-HTP)

Vargas, Marta A.; Deive, Francisco J.; Álvarez, María S.; Longo, María A.; Rodríguez, Ana; Bernal, Claudia; Martínez, Ronny

doi:10.1016/j.enzmictec.2021.109975

Cited by 8 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Elevated levels of tryptophan in patients with diabetes after drug treatment suggest that tryptophan and diabetes may be closely related [5], and tryptophan was thought to regulate blood glucose levels in another study. In this experiment, inulin mainly enhanced the generation of 5-hydroxy-ltryptophan (5-HTP), which is a precursor substance of serotonin that regulates a series of metabolic and psychological functions, in the metabolism of tryptophan [53]. In a previous study, 5-HTP was linked to weight loss by suppressing appetite [54], but this result is controversial.…”

Section: Discussionmentioning

confidence: 91%

Medium-chain inulin alleviates the obesity and improves systemic health in diet-induced obese mice via mediating the gut microbiome and metabolome

Wang,

Kim,

Cui

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Inulin is well known as a natural dietary fiber with multiple beneficial effects on promoting the physiological and intestinal health of hosts. However, it is unclear whether medium-chain inulin can inhibit high-fat diet (HFD)-induced obesity in mice and its mechanism(s) that underlie such effects. In this study, we tested the hypothesis that medium-chain inulin consumption can counteract the obesity in HFD-fed mice by selectively modulating the gut microbiota, metabolome, and metabolic pathways. Results The results shown that the medium-chain inulin is a polymer primarily consisting of fructose linked 2,1 glycosidic bonds with an average degree polymerization of 12. Among them, molecules of different chain lengths form spherical or cross-linked network spatial structures by folding and coiling. Medium-chain inulin supplementation (3% or 5%) reduced lipid deposition in tissues, ameliorated intestinal health, and thus prevented diet-induced obesity in mice. Furthermore, dietary inclusion of inulin shaped a significantly different microbial community by specially increasing the abundances of Bacteroidota, Faecalibaculum, Bifidobacterium, Parasutterella, Lactobacillaceae, Bacteroides, Clostridium_sensu_stricto_1, and Akkermansia in inulin-supplemented groups compared with the HFD group. Additionally, dietary inulin supplementation enhanced the concentrations of nicotinamide, indole-3-acetate, 5-hydroxytryptophan, maltose, taurocholic acid, aldosterone 5α-dihydrotestosterone, and corticosterone in gut content of HFD-fed mice, and thus modulated certain energy utilization-related metabolic pathways. Especially, those significantly modulated microbes and metabolites by inulin supplementation positively correlated with ameliorated healthy parameters. Therefore, medium-chain inulin inhibits HFD-induced obesity by specifically modulating key gut microbes and metabolites, thereby affecting the inulin-gut microbiota/metabolome-tissue (phenotype) axis. Conclusions Taken together, dietary inulin supplementation improved the gut barrier and physiological health, while alleviated HFD-induced obesity and complications of mice, including fat deposition and metabolic disorders, etc. This beneficial effect was attributed to the fact that feeding medium-chain inulin could specifically promote certain key beneficial gut bacteria and metabolites, and thus arouse energy utilization-related metabolic pathways alteration. Thus, medium-chain inulin can be employed as a potential functional food or therapeutic agent to prevent or treat diet-induced obesity in the host.

show abstract

Section: Discussionmentioning

confidence: 91%

Medium-chain inulin alleviates the obesity and improves systemic health in diet-induced obese mice via mediating the gut microbiome and metabolome

Wang,

Kim,

Cui

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…C-H hydroxylation is a promising strategy to synthesize hydroxy amino acids (HAAs), which are widely used in the chemical, feed, and medicinal industries [16]. For example, 5-hydroxytryptophan is the functional precursor for the synthesis of serotonin, exhibiting important pharmaceutical activity [17]. Moreover, N-hydroxy-pipecolic acid is an important systemically acquired resistance signal molecule [18].…”

Section: Introductionmentioning

confidence: 99%

Engineering an Artificial Pathway to Improve the Bioconversion of Lysine into Chiral Amino Alcohol 2-Hydroxycadaverine Using a Semi-Rational Design

Cheng,

Xiao,

Luo

et al. 2024

Fermentation

View full text Add to dashboard Cite

Amino alcohols are important compounds that are widely used in the polymer and pharmaceutical industry, particularly when used as chiral scaffolds in organic synthesis. The hydroxylation of polyamide polymers may allow crosslinking between molecular chains through the esterification reactions of hydroxyl and carboxyl groups. Therefore, this may alter the functional properties of polyamide polymers. 2-hydroxycadaverine (2HyC), as a new type of chiral amino alcohol, has potential applications in the pharmaceutical, chemical, and polymer industries. Currently, 2HyC production has only been realized via pure enzyme catalysis or two-stage whole-cell biocatalysis, which faces great challenges for scale-up production. However, the use of a cell factory is very promising for the production of 2HyC in industrial applications. Here, we designed and constructed a promising artificial pathway in Escherichia coli for producing 2HyC from biomass-derived lysine. This biosynthesis route expands the lysine catabolism pathway and employs two enzymes to sequentially convert lysine into 2HyC. However, the catalytic activity of wild-type pyridoxal phosphate-dependent decarboxylase from Chitinophage pinensis (DCCp) toward 3-hydroxylysine is lower, resulting in the lower production of 2HyC. Thus, the higher catalytic activity of DCCp is desired for low-cost and expanded industrial applications of 2HyC. To improve the catalytic activity of DCCp, a mutant library of DCCp was first built using a semi-rational design. The Kcat/Km of mutant DCCp (R53D/V94I) increased by 63%. A titer of 359 mg/L 2HyC was produced in shake flasks, with a 2HyC titer increase of 54% compared to control strain ML101. The results show that the production of 2HyC was effectively increased through a semi-rational design strategy. These findings lay the foundation for the development and utilization of renewable resources to produce 2HyC in microorganisms via an efficient, green, and sustainable biosynthetic strategy for further industrial application.

show abstract

Advances in the microbial synthesis of the neurotransmitter serotonin

Wang

Chen

Zhang

et al. 2023

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Effect of process parameters and surfactant additives on the obtained activity of recombinant tryptophan hydroxylase (TPH1) for enzymatic synthesis of 5-hydroxytryptophan (5-HTP)

Cited by 8 publications

References 28 publications

Medium-chain inulin alleviates the obesity and improves systemic health in diet-induced obese mice via mediating the gut microbiome and metabolome

Medium-chain inulin alleviates the obesity and improves systemic health in diet-induced obese mice via mediating the gut microbiome and metabolome

Engineering an Artificial Pathway to Improve the Bioconversion of Lysine into Chiral Amino Alcohol 2-Hydroxycadaverine Using a Semi-Rational Design

Advances in the microbial synthesis of the neurotransmitter serotonin

Contact Info

Product

Resources

About