Effect of Product Inhibition on Xylose Fermentation to Ethanol by Saccharomyces cerevisae 424A (LNH-ST)

Athmanathan, Arun; Sedlák, Miloš; Ho, Nancy W. Y.; Mosier, Nathan S.

doi:10.13031/2013.36315

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…Saccharomyces cerevisiae 424A(LNH-ST), a recombinant yeast strain capable of the co-fermentation of glucose and xylose, was utilized in the experiments [20,22,33]. …”

Section: Methodsmentioning

confidence: 99%

Effect of salts on the Co-fermentation of glucose and xylose by a genetically engineered strain of Saccharomyces cerevisiae

Casey

Mosier

Adamec

et al. 2013

Biotechnol Biofuels

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BackgroundA challenge currently facing the cellulosic biofuel industry is the efficient fermentation of both C5 and C6 sugars in the presence of inhibitors. To overcome this challenge, microorganisms that are capable of mixed-sugar fermentation need to be further developed for increased inhibitor tolerance. However, this requires an understanding of the physiological impact of inhibitors on the microorganism. This paper investigates the effect of salts on Saccharomyces cerevisiae 424A(LNH-ST), a yeast strain capable of effectively co-fermenting glucose and xylose.ResultsIn this study, we show that salts can be significant inhibitors of S. cerevisiae. All 6 pairs of anions (chloride and sulfate) and cations (sodium, potassium, and ammonium) tested resulted in reduced cell growth rate, glucose consumption rate, and ethanol production rate. In addition, the data showed that the xylose consumption is more strongly affected by salts than glucose consumption at all concentrations. At a NaCl concentration of 0.5M, the xylose consumption rate was reduced by 64.5% compared to the control. A metabolomics study found a shift in metabolism to increased glycerol production during xylose fermentation when salt was present, which was confirmed by an increase in extracellular glycerol titers by 4 fold. There were significant differences between the different cations. The salts with potassium cations were the least inhibitory. Surprisingly, although salts of sulfate produced twice the concentration of cations as compared to salts of chloride, the degree of inhibition was the same with one exception. Potassium salts of sulfate were less inhibitory than potassium paired with chloride, suggesting that chloride is more inhibitory than sulfate.ConclusionsWhen developing microorganisms and processes for cellulosic ethanol production, it is important to consider salt concentrations as it has a significant negative impact on yeast performance, especially with regards to xylose fermentation.

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“…Saccharomyces cerevisiae 424A(LNH-ST), a recombinant yeast strain capable of the co-fermentation of glucose and xylose, was utilized in the experiments [20,22,33]. …”

Section: Methodsmentioning

confidence: 99%

Effect of salts on the Co-fermentation of glucose and xylose by a genetically engineered strain of Saccharomyces cerevisiae

Casey

Mosier

Adamec

et al. 2013

Biotechnol Biofuels

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“…To understand the impact of the ethanol specific production rate on the overall flow rate of the products, the fermentation rates were implemented in the reactor design kinetics equations. Cell growth, glucose consumption, and ethanol production rates were extracted from Pearl using the assumptions and coefficients from later studies . The assumptions in this study are mentioned in the appropriate sections of the manuscript.…”

Section: Methodsmentioning

confidence: 99%

“…Cell growth, glucose consumption, and ethanol production rates were extracted from Pearl 26 using the assumptions and coefficients from later studies. 20,27 The assumptions in this study are mentioned in the appropriate sections of the manuscript.…”

Section: Ethyl Acetatementioning

confidence: 99%

“…As a previous study suggested, adopting an LLE system is an efficient technique to remove known fermentation inhibitors regardless of the biomass type and the species of fermenting microorganism. Though exact kinetics of the inhibition of acetic acid and other known inhibitors on the ethanol producing microorganisms requires additional study, the straightforward, linear equations that have been used in these calculations are expandable for use at various flow rates, with different materials of construction, a range of years of operation, and different compositions of feedstocks. The comparative analysis that has been incorporated into this systematic study clarifies the most important parameters that determine whether LLE can be a viable addition to an existing biorefinery.…”

Section: Introductionmentioning

confidence: 99%

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Techno‐economic analysis for incorporating a liquid–liquid extraction system to remove acetic acid into a proposed commercial scale biorefinery

Aghazadeh

Engelberth

2016

Biotechnology Progress

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Mitigating the effect of fermentation inhibitors in bioethanol plants can have a great positive impact on the economy of this industry. Liquid-liquid extraction (LLE) using ethyl acetate is able to remove fermentation inhibitors-chiefly, acetic acid-from an aqueous solution used to produce bioethanol. The fermentation broth resulting from LLE has higher performance for ethanol yield and its production rate. Previous techno-economic analyses focused on second-generation biofuel production did not address the impact of removing the fermentation inhibitors on the economic performance of the biorefinery. A comprehensive analysis of applying a separation system to mitigate the fermentation inhibition effect and to provide an analysis on the economic impact of removal of acetic acid from corn stover hydrolysate on the overall revenue of the biorefinery is necessary. This study examines the pros and cons associated with implementing LLE column along with the solvent recovery system into a commercial scale bioethanol plant. Using details from the NREL-developed model of corn stover biorefinery, the capital costs associated with the equipment and the operating cost for the use of solvent were estimated and the results were compared with the profit gain due to higher ethanol production. Results indicate that the additional capital will add 1% to the total capital and manufacturing cost will increase by 5.9%. The benefit arises from the higher ethanol production rate and yield as a consequence of inhibitor extraction and results in a $0.35 per gallon reduction in the minimum ethanol selling price (MESP). © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:971-977, 2016.

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Bioethanol from Lignocellulosic Biomass

Zhao

Bai

et al. 2011

Biotechnology in China III: Biofuels and Bioenergy

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China is suffering from a sustained shortage of crude oil supply, making fuel ethanol and other biofuels alternative solutions for this issue. However, taking into account the country's large population and dwindling arable land due to rapid urbanization, it is apparent that current fuel ethanol production from grain-based feedstocks is not sustainable, and lignocellulosic biomass, particularly agricultural residues that are abundantly available in China, is the only choice for China to further expand its fuel ethanol production, provided economically viable processes can be developed. In this chapter, cutting edge progress in bioethanol is reviewed, with a focus on the understanding of the molecular structure of the feedstock, leading pretreatment technologies, enzymatic hydrolysis of the cellulose component and strategies for the co-fermentation of the C5 and C6 sugars with engineered microorganisms. Finally, process integration and optimization is addressed with a case study on the COFCO Corporation's pilot plant, and challenges and perspectives for commercial production of bioethanol are highlighted.

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Effect of Product Inhibition on Xylose Fermentation to Ethanol by Saccharomyces cerevisae 424A (LNH-ST)

Cited by 7 publications

References 35 publications

Effect of salts on the Co-fermentation of glucose and xylose by a genetically engineered strain of Saccharomyces cerevisiae

Effect of salts on the Co-fermentation of glucose and xylose by a genetically engineered strain of Saccharomyces cerevisiae

Techno‐economic analysis for incorporating a liquid–liquid extraction system to remove acetic acid into a proposed commercial scale biorefinery

Bioethanol from Lignocellulosic Biomass

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