Effect of prolactin on pathways of glucose oxidation in explants from rabbit mammary gland

Bolton, Christine E.; Bolton, A.E.

doi:10.1016/0014-5793(70)80348-9

Cited by 7 publications

(8 citation statements)

References 15 publications

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“…By contrast, glucosephosphate isomerase, an enzyme of the glycolytic pathway for glucose metabolism, was strongly stimulated by insulin alone over the period of 24 to 72 h in culture (Rivera & Cummins, 1971). These results accord well with those of Bolton & Bolton (1970) who obtained stimulation of pentose phosphate pathway activity in rabbit mammary gland with prolactin in the presence of insulin + corticosterone (see below).…”

Section: Protein and Enzyme Synthesissupporting

confidence: 87%

“…The grid may be overlaid with lens paper, agar, rayon or nylon fibre (Moscona et al 1965) but lobulo-alveolar tissue of mammary gland survives satisfactorily if placed directly on the metal. A culture vessel of this type is illustrated in Plate 1 (a) and a simplified version of it, suitable for larger-scale cultures for biochemical studies, is shown in Plate 1(6) (Bolton, 1970;Bolton & Bolton, 1970).…”

Section: Types Of Culture Vesselmentioning

confidence: 99%

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Section A. Physiology. Organ culture techniques and the study of hormone effects on the mammary gland

Forsyth¹

1971

Journal of Dairy Research

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Section: Protein and Enzyme Synthesissupporting

confidence: 87%

Section: Types Of Culture Vesselmentioning

confidence: 99%

Section A. Physiology. Organ culture techniques and the study of hormone effects on the mammary gland

Forsyth¹

1971

Journal of Dairy Research

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“…Fatty acidsynthesis by mammary explantsfrom rabbits pseudopregnant for 11 days Most studies in vitro on the response of rabbit mammary gland to hormones have used tissue from pseudopregnant rabbits (Bolton & Bolton, 1970;Falconer & Fiddler, 1970;Bolton, 1971;Fiddler et al, 1971;Delouis & Denamur, 1972). This tissue readily responds to insulin, corticosterone and prolactin by increasing the overall rate of fatty acid synthesis and the proportion of medium-chain fatty acids synthesized .…”

Section: Resultsmentioning

confidence: 99%

Interactions of insulin, corticosterone and prolactin in promoting milk-fat synthesis by mammary explants from pregnant rabbits

Forsyth¹,

Strong

Dils

1972

Biochemical Journal

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1. The rate of fatty acid synthesis by mammary explants from rabbits pregnant for 16 days or from rabbits pseudopregnant for 11 days was stimulated up to 15-fold by culturing for 2-4 days with prolactin. This treatment initiated the predominant synthesis of C(8:0) and C(10:0) fatty acids, which are characteristic of rabbit milk. 2. Inclusion of insulin in the culture medium increased the rate of synthesis of these medium-chain fatty acids. By contrast the inclusion of corticosterone led to the predominant synthesis of long-chain fatty acids. When explants were cultured for 2-4 days with insulin, corticosterone and prolactin, the rate of fatty acid synthesis increased up to 42-fold, but both medium- and long-chain fatty acids were synthesized. 3. These results show that the stimulus to mammary-gland lipogenesis and the initiation of synthesis of medium-chain fatty acids observed between days 16 and 23 of pregnancy in the rabbit can be simulated in vitro by prolactin alone. 4. When mammary explants from rabbits pregnant for 23 days were cultured for 2 days with insulin, corticosterone and prolactin, the rate of fatty acid synthesis increased fivefold, but there was a preferential synthesis of long-chain fatty acids. Culture with prolactin alone had little effect on the rate or pattern of fatty acids synthesized. 5. The results are compared with findings in vivo on the control of lipogenesis in the rabbit mammary gland, and are contrasted with the known effects of hormones in vitro on the mammary gland of the mid-pregnant mouse.

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“…Bovine growth hormone has no significant effect on either component of the enzyme. Prolactin is known to act directly on the mammary glands of pregnant or pseudopregnant rabbits to produce structural and biochemical changes associated with lactation in intact animals (Chadwick, 1962;Heitzman, 1968;Falconer & Fiddler, 1970;Fiddler et al, 1971) and in cultured explants (Barnawell, 1965;Bolton & Bolton, 1970). Thus in the work reported in the present paper it can be assumed that prolactin stimulates lactose synthesis by such a direct action.…”

Section: Discussionmentioning

confidence: 89%

The effect of hypophysectomy and subsequent replacement therapy with sheep prolactin or bovine growth hormone on the lactose synthetase activity of rabbit mammary gland

Jones¹,

Cowie²

1972

Biochemical Journal

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1. The effects of hypophysectomy and replacement therapy with sheep prolactin and bovine growth hormone on the lactose synthetase activity of the mammary glands of lactating rabbits were studied. 2. There was an approximately fourfold decline in the lactose synthetase activity of homogenates calculated on a DNA basis within 6-7 days of hypophysectomy. Prolactin reversed this decline but growth hormone had no effect. 3. Changes in the properties of a particulate fraction isolated from the glands indicated that a decline in the effective concentration of alpha-lactalbumin was one factor contributing to the decreased lactose synthetase activity after hypophysectomy. 4. As the changes in lactose output produced by hypophysectomy and prolactin therapy are much greater than the changes in total lactose synthetase activity it is concluded that the activity of this enzyme is not the main factor controlling lactose output under these conditions.

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Effect of prolactin on pathways of glucose oxidation in explants from rabbit mammary gland

Cited by 7 publications

References 15 publications

Section A. Physiology. Organ culture techniques and the study of hormone effects on the mammary gland

Section A. Physiology. Organ culture techniques and the study of hormone effects on the mammary gland

Interactions of insulin, corticosterone and prolactin in promoting milk-fat synthesis by mammary explants from pregnant rabbits

The effect of hypophysectomy and subsequent replacement therapy with sheep prolactin or bovine growth hormone on the lactose synthetase activity of rabbit mammary gland

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