Effect of prolonged exposure to sublethal concentrations of DDT and DDE on protein expression in human pancreatic beta cells

Pavlíková, Nela; Smetana, Pavel; Halada, Petr; Kovář, Jan

doi:10.1016/j.envres.2015.06.046

Cited by 30 publications

(28 citation statements)

References 41 publications

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“…Different authors have proved DDT to be hazardous to humans by using different tests [50][51][52][53][54][55]. Shaw et al [56] presented data on pesticide residues in milk from Indonesian women which demonstrated general exposure to p,p'-DDT and hexachlorobenzene (HCB).…”

Section: Toxicity Among Humansmentioning

confidence: 99%

Toxic Effects of Organochlorine Pesticides: A Review

Singh¹

2016

AJBIO

100

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This study is aimed at compiling the toxicological aspects of organochlorine compounds (OCCs) including pesticides among different animal models. Tests on animal species like Mean Probable Effect Concentration Quotient (PEC-Q) test, DNA repair assays and histopathological examinations have shown positive results for the toxicity of organochlorine pesticides. The results were observed on different animals including fishes, furseals, frogs, rats, bats and humans. In fishes, endosulfan is found to have acute toxicities. Similarly, organophosphorus, synthetic pyrethroids and microbial insecticides were also found to show their toxic effects. Some compounds such as lambda-cyhalothrin showed a very high toxicity on fish followed by fenvalerate, deltamethrin and cypermethrin. Exposure to OCCs can impart foe cell death by inducing Mitogen Activated Protein Kinase Pathway (MAPK) which is associated with cell growth differentiation and apoptosis. On studying the genotoxic effects of OCCs on germ cells of mouse, it was observed that a lethal mutation can occur just after one mating interval. Increase in the number of micronucleated cells has also been seen after OCC exposure. Neuro-behavioural studies on rats showed the presence of tremors caused by chlordecone and p,p'DDT. Tumorigenicity by organochlorine pesticides is seen to be an epigenetic mechanism in a DNA repair assay. Acute poisoning was seen in liver, kidney and testis of albino rat due to the toxic effect of dursban and DDT. Great declines in populations have been seen due to OCC toxicity in different animal species. Direct and indirect exposure to these compounds should be reduced so as to minimize the possible health hazards.

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Section: Toxicity Among Humansmentioning

confidence: 99%

Toxic Effects of Organochlorine Pesticides: A Review

Singh¹

2016

AJBIO

100

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“…For our study, we wanted to establish a concentration of p,p‘- DDT that would kill approximately 20 % of cells after 24 hours of exposure, so the remaining 80 % of cells would provide us with enough proteins for 2-D electrophoresis. Tested concentrations were chosen based on our previous results (6). After 24 hours of exposure, the two lower concentrations of p,p‘- DDT (100 μM and 125 μM) caused only a non-significant decrease in cell viability when compared with control (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Many compounds have the potential to harm pancreatic beta-cells and disrupt glucose homeostasis in the human organism (1). Such compounds include pharmaceuticals like pentamidine (2), or fluoxetine (SSRI antidepressant) (3) or saturated fatty acids palmitate (4), or stearate (5); and potentially also organochlorine pollutants, such as the now-banned pesticide DDT (6, 7). Even decades after most of the countries banned its use, DDT and its metabolites persist in the environment (8, 9) and represent a threat to living organisms (10, 11).…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, we used 2-D electrophoresis coupled to mass spectrometry to find proteins possibly involved in mechanisms mediating prolonged (1 month) effect of non-lethal doses of organochlorine pollutant p,p ’-DDT in pancreatic beta-cells (6, 18). In our present study, we aimed to find markers of acute toxicity of lethal doses of p,p ’-DDT in human pancreatic beta-cells NES2Y, also employing 2-D electrophoresis.…”

Section: Introductionmentioning

confidence: 99%

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Markers of Acute Toxicity in Pancreatic Beta-Cells Exposed to Lethal Doses of the Organochlorine Pollutant DDT Determined by a Proteomic Approach

Pavlíková

Šrámek

Jelínek

et al. 2020

Preprint

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Many compounds have the potential to harm pancreatic beta-cells; organochlorine pollutants belong to those compounds. In this work, we aimed to find markers of acute toxicity of p,p‘-DDT among proteins expressed in human pancreatic beta-cells NES2Y and visible at 2-D electrophoresis. We exposed NES2Y cells to a lethal dose of p,p‘-DDT (150 μM) for 24 hours and 30 hours and determined changes in protein expression using 2-D electrophoresis. We also stained the cells exposed to p,p‘-DDT to visualize the altered phenotype of the exposed cells. Among proteins with changed expression, we identified proteins involved in ER stress (GRP78, and endoplasmin), mitochondrial proteins (GRP75, ECHM, IDH3A, NDUS1, and NDUS3), proteins with potential to change the cell morphology (EFHD2, TCPA, NDRG1, and ezrin), and some other proteins (HNRPF, HNRH1, K2C8, vimentin, PBDC1, EF2, PCNA, biliverdin reductase, G3BP1, FRIL, and HSP27). These proteins can be used as markers of acute DDT toxicity.

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“…Among five subclasses of POPs, organochlorine pesticides showed a strong dose-response relation with prevalence of peripheral neuropathy, adjusted odds ratios were 1.0, 3.6, and 7.3 (P for trend <0.01), respectively, across three categories of serum concentrations of organochlorine pesticides [108]. Studies show that diabetics with higher levels of POPs have several times the risk of diabetic nephropathy [109][110][111][112].…”

Section: The Impact Of Pop Exposure On Laboratory Animalsmentioning

confidence: 99%

The Prevention and Treatment of Type 2 Diabetes Mellitus with a Plant-Based Diet

Strombom¹,

Rose²

2017

EMIJ

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Those following a plant-based diet have a 78% reduction risk of Type II diabetes mellitus (T2DM), as well as a 56% reduced risk of metabolic syndrome and a lower average BMI, 22.4 for men and 21.8 for women. Vegetarians have less skeletal intramyocellular lipids, and better myocellular glucose disposal and mitochondrial function, and therefore have less peripheral insulin resistance. Vegetarians and vegans also have a much better inflammatory status, indicated by lower levels of inflammatory markers such as CRP and inflammatory adipocytokines such as IL-6, leptin, and higher levels of anti-inflammatory adipocytokines such as adiponectin. Those following a plant-based diet consume much less persistent organic pollutants (POPs) which have been shown to cause beta-cell mitochondrial dysfunction. Finally, the gut microbiome of vegetarians has been shown to play a role in reducing insulin resistance and the level of inflammation in the body, and consequently their risk of T2DM. Interventional studies show a reduction of HbA1C by as much as 2.4 percentage pts, which is more than is usually achieved with Metformin. Other clinical variables also show improvement such as reductions in BMI, total and LDL cholesterol and hsCRP. Studies show compliance rates are good, ranging from 84% to 99%. Medications should be titrated as the patient shows improvements. Treatment with a plantbased diet has no contraindications or adverse reactions. Keywords:

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Effect of prolonged exposure to sublethal concentrations of DDT and DDE on protein expression in human pancreatic beta cells

Cited by 30 publications

References 41 publications

Toxic Effects of Organochlorine Pesticides: A Review

Toxic Effects of Organochlorine Pesticides: A Review

Markers of Acute Toxicity in Pancreatic Beta-Cells Exposed to Lethal Doses of the Organochlorine Pollutant DDT Determined by a Proteomic Approach

The Prevention and Treatment of Type 2 Diabetes Mellitus with a Plant-Based Diet

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