Effect of propolis extract on hematotoxicity and histological changes induced by Salmonella enterica serovar Typhimurium in BALB/c mice

Abstract

Typhoid fever, a life-threatening disease, causes several pathological changes due to the involvement of one or more organs. In the present study, the effect of typhoid on hematological indices and spleen histology in infected mice were investigated. The ameliorative efficacy of the ethanolic extract of propolis was tested and compared with treatment with the typhoid drug,cefixime. BALB/c mice were divided into six groups: group1 was the normal control, group 2 was infected with Salmonella enteric serovar Typhimurium, group 3 was Salmonella infected and treated with cefixime, groups4 and 5 were Salmonella-infected mice treated with 100 mg and 300 mg of propolis respectively. Group 6 was given only 300 mg propolis without infection. Mice were killed after completion of treatment and examination of spleen histology and hematological studies were performed. Significant differences were observed in the propolis-treated group of mice as compared to the infected group without antibiotic, further confirming the ameliorative effect of propolis on Salmonella enterica serovar Typhimurium-induced toxicity in mice.

Studies on the therapeutic effect of propolis along with standard antibacterial drug in Salmonella enterica serovar Typhimurium infected BALB/c mice

“…Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were also calculated [12]. …”

The Effects of Melatonin and Propolis on Markers of Inflammation, Oxidative Stress, Clinical Outcomes, and Survival Rate in Patients with Primary Sepsis Hospitalized in Intensive Care Unit: A Randomized Clinical Trial

“…A review study have reported the antibacterial activity of propolis and its extracts against gram-positive and gram-negative bacterial strains and found that propolis has antibacterial activity against a wide range of gram-positive bacteria, but this activity is more limited in the gram-negative bacilli (54). An animal study showed that propolis could reduce the rate of infection caused by Salmonella bacteria, as well as keep WBC and neutrophil in normal levels (55). In one animal study that conducted by El-Aidy et al, propolis was able to maintain leukocyte and neutrophil at normal levels compared to other groups (56).…”

Effects of Melatonin and Propolis Supplementation on Inflammation, Oxidative Stress, and Clinical Outcomes in Patients with Primary Pneumosepsis: A Randomized Controlled Clinical Trial

“…A review in this regard reported the antibacterial activity of propolis and its extracts against bacterial strains, indicating that propolis has antibacterial activity against a wide range of gram-positive bacteria, while the activity is rather limited in the case of gram-negative bacilli [55]. On the other hand, an animal study showed that propolis could reduce the rate of infection caused by Salmonella enterica serovar typhimurium, while maintaining normal WBC and neutrophils [56]. An animal study conducted by El-Aidy et al [57] in this regard also indicated that propolis maintained the normal levels of leukocytes and neutrophils Complement Med Res 2022;29:275-285 DOI: 10.1159/000523766 compared to the untreated group.…”