Effect of pulsed electromagnetic field therapy on the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells

Lu, Tian Jian; Yx, Huang; Zhang, C; Mx, Chai; Zhang, J

doi:10.4238/2015.september.28.5

Cited by 21 publications

(15 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The work of Lu et al showed stimulatory effects of f = 20 Hz ELF-PEMF on ALP and Osteocalcin levels in rat-derived mesenchymal stem cells. In their experiments, ELF-PEMF exposure even suppressed adipogenic differentiation [79]. In line with this, both the study of Martino et al [80] and the study of Hannay et al [81] showed f = 15 Hz ELF-PEMF stimulatory effects on ALP activity (within few hours) and matrix mineralization (within 2-4 days) of SaOs-2 cells.…”

Section: Elf-pemf Effects On Bone Cell Functionmentioning

confidence: 64%

Translational Insights into Extremely Low Frequency Pulsed Electromagnetic Fields (ELF-PEMFs) for Bone Regeneration after Trauma and Orthopedic Surgery

Ehnert

Schröter

Aspera-Werz

et al. 2019

JCM

View full text Add to dashboard Cite

The finding that alterations in electrical potential play an important role in the mechanical stimulation of the bone provoked hype that noninvasive extremely low frequency pulsed electromagnetic fields (ELF-PEMF) can be used to support healing of bone and osteochondral defects. This resulted in the development of many ELF-PEMF devices for clinical use. Due to the resulting diversity of the ELF-PEMF characteristics regarding treatment regimen, and reported results, exposure to ELF-PEMFs is generally not among the guidelines to treat bone and osteochondral defects. Notwithstanding, here we show that there is strong evidence for ELF-PEMF treatment. We give a short, confined overview of in vitro studies investigating effects of ELF-PEMF treatment on bone cells, highlighting likely mechanisms. Subsequently, we summarize prospective and blinded studies, investigating the effect of ELF-PEMF treatment on acute bone fractures and bone fracture non-unions, osteotomies, spinal fusion, osteoporosis, and osteoarthritis. Although these studies favor the use of ELF-PEMF treatment, they likewise demonstrate the need for more defined and better controlled/monitored treatment modalities. However, to establish indication-oriented treatment regimen, profound knowledge of the underlying mechanisms in the sense of cellular pathways/events triggered is required, highlighting the need for more systematic studies to unravel optimal treatment conditions.

show abstract

Section: Elf-pemf Effects On Bone Cell Functionmentioning

confidence: 64%

Translational Insights into Extremely Low Frequency Pulsed Electromagnetic Fields (ELF-PEMFs) for Bone Regeneration after Trauma and Orthopedic Surgery

Ehnert

Schröter

Aspera-Werz

et al. 2019

JCM

View full text Add to dashboard Cite

show abstract

“…It is well known that differentiation of BMSCs is crucial to SONFH. A variety of physical, chemical or biological factors have been confirmed to affect the differentiation of BMSCs, such as pulsed electromagnetic fields [33], pravastatin [34], and miR-27a [35]. Furthermore, Runx2 is known to be essential in the process of osteogenic differentiation [36] and transdifferentiation [37] of BMSCs.…”

Section: Discussionmentioning

confidence: 99%

Silencing MicroRNA-137-3p, which Targets RUNX2 and CXCL12 Prevents Steroid-induced Osteonecrosis of the Femoral Head by Facilitating Osteogenesis and Angiogenesis

Kong¹,

Zuo²,

Wang³

et al. 2020

Int. J. Biol. Sci.

View full text Add to dashboard Cite

The main pathogenesis of steroid-induced osteonecrosis of the femoral head (SONFH) includes decreased osteogenic capacity of bone marrow-derived mesenchymal stem cells (BMSCs) and damaged blood supply to the femoral head. MicroRNAs (miRNAs) have been shown to play prominent roles in SONFH development. However, there is no report that a specific miRNA targeting two genes in two different pathogenic pathways has been applied to this disease. The present study investigated the effects of transplantation of miR-137-3p-silenced BMSCs on the prevention and early treatment of SONFH. First, western blotting and dual luciferase assays were employed to verify that miR-137-3p directly targets Runx2 and CXCL12. Then, silencing of miR-137-3p was found to facilitate osteogenic differentiation of BMSCs, which was confirmed by alkaline phosphatase (ALP) staining, alizarin red staining and qRT-PCR. Silencing of miR-137-3p also promoted angiogenesis by human umbilical vein endothelial cells (HUVECs) in the presence or absence of glucocorticoids. Thereafter, overexpression of Runx2 and CXCL12 without the 3′ untranslated region (3′UTR) partially rescued the effects of miR-137-3p on osteogenesis and angiogenesis, respectively. This finding further supported the hypothesis that miR-137-3p exerts its functions partly by regulating the genes, Runx2 and CXCL12. We also demonstrated that SONFH was partially prevented by transplantation of miR-137-3p-silenced BMSCs into a rat model. Micro-CT and histology showed that the transplantation of miR-137-3p-silenced BMSCs significantly improved bone regeneration. Additionally, the results of enzyme-linked immunosorbent assays (ELISA) and flow cytometry suggested that stromal cell-derived factor-1α (SDF-1α) and endothelial progenitor cells (EPCs) participated in the process of vascular repair. Taken together, these findings show that silencing of miR-137-3p directly targets the genes, Runx2 and CXCL12, which can play critical roles in SONFH repair by facilitating osteogenic differentiation and mobilizing EPCs.Key words: steroid-induced osteonecrosis of the femoral head, miR-137-3p, bone marrow-derived mesenchymal stem cells, CXCL12 (SDF-1α), Runx2, endothelial progenitor cells

show abstract

“…PTH increases intestinal calcium absorption and decreases efflux of calcium from bone as a calcium regulating hormone, and increases bone formation by promoting osteoblastogenesis and osteoblast survival as a natural anabolic agent [Esbrit and Alcaraz, ; Gardinier et al, ]. It was reported that an 8‐day Spacelab‐2 mission flight and long term bed rest caused a fall of PTH which was accompanied by decreased bone formation [Zerwekh et al, ; Lu et al, ]. In the current experiment, the serum PTH level of HLS + PEMFs group was kept at a similar level with the control, indicating that PEMFs may prevent bone loss by maintaining the systemic PTH level (Fig.…”

Section: Discussionmentioning

confidence: 51%

Pulsed electromagnetic fields prevented the decrease of bone formation in hindlimb‐suspended rats by activating sAC/cAMP/PKA/CREB signaling pathway

Tian

et al. 2018

Bioelectromagnetics

View full text Add to dashboard Cite

Microgravity is one of the main threats to the health of astronauts. Pulsed electromagnetic fields (PEMFs) have been considered as one of the potential countermeasures for bone loss induced by space flight. However, the optimal therapeutic parameters of PEMFs have not been obtained and the action mechanism is still largely unknown. In this study, a set of optimal therapeutic parameters for PEMFs (50 Hz, 0.6 mT 50% duty cycle and 90 min/day) selected based on high-throughput screening with cultured osteoblasts was used to prevent bone loss in rats induced by hindlimb suspension, a commonly accepted animal model to simulate the space environment. It was found that hindlimb suspension for 4 weeks led to significant decreases in femoral and vertebral bone mineral density (BMD) and their maximal loads, severe deterioration in bone micro-structure, and decreases in levels of bone formation markers and increases in bone resorption markers. PEMF treatment prevented about 50% of the decreased BMD and maximal loads, preserved the microstructure of cancellous bone and thickness of cortical bone, and inhibited decreases in bone formation markers. Histological analyses revealed that PEMFs significantly alleviated the reduction in osteoblast number and inhibited the increase in adipocyte number in the bone marrow. PEMFs also blocked decreases in serum levels of parathyroid hormone and its downstream signal molecule cAMP, and maintained the phosphorylation levels of protein kinase A (PKA) and cAMP response element-binding protein (CREB). The expression level of soluble adenylyl cyclases (sAC) was also maintained. It therefore can be concluded that PEMFs partially prevented the bone loss induced by weightless environment by maintaining bone formation through signaling of the sAC/cAMP/PKA/ CREB pathway. Bioelectromagnetics. 39:569-584, 2018.

show abstract

Effect of pulsed electromagnetic field therapy on the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells

Cited by 21 publications

References 19 publications

Translational Insights into Extremely Low Frequency Pulsed Electromagnetic Fields (ELF-PEMFs) for Bone Regeneration after Trauma and Orthopedic Surgery

Translational Insights into Extremely Low Frequency Pulsed Electromagnetic Fields (ELF-PEMFs) for Bone Regeneration after Trauma and Orthopedic Surgery

Silencing MicroRNA-137-3p, which Targets RUNX2 and CXCL12 Prevents Steroid-induced Osteonecrosis of the Femoral Head by Facilitating Osteogenesis and Angiogenesis

Pulsed electromagnetic fields prevented the decrease of bone formation in hindlimb‐suspended rats by activating sAC/cAMP/PKA/CREB signaling pathway

Contact Info

Product

Resources

About