Effect of radium treatment on activity and distribution of some enzymes in cancers of the human cervix uteri

Abstract

Fr%:m the D c p r m e n t of I$iochcmistry, arid the L'ni-

Vaginal Fluid Ribonuclease Activity in the Diagnosis of Cervical Cancer

“…Such discharges would in any case necessitate thorough gynaecological examination. RNAase activity did not correlate well with the clinical stage of the disease This is not surprising, since the tissue levels are not a good index of the degree of malignancy of the tumour (Goldberg and Pitts, 1966), and tumour RNAase activity increases significantly after radiotherapy (Goldberg, Ayre and Pitts, 1967).…”

“…Two of the false positives were suffering from trichomonal vaginitis, a condition associated with falsepositive results for both P-glucuronidase and 6-phosphogluconate dehydrogenase (Hoffman and Merritt, 1965;Bell and Egerton, 1965). RNAase activity did not correlate well with the clinical stage of the disease This is not surprising, since the tissue levels are not a good index of the degree of malignancy of the tumour (Goldberg and Pitts, 1966), and tumour RNAase activity increases significantly after radiotherapy (Goldberg, Ayre and Pitts, 1967). One of the false-negative cases had a heavily blood-stained discharge, while the other had a purulent discharge.…”

Effect of radiotherapy upon enzymes of the glycolytic and related pathways in human uterine cancer

“…Much evidence implicates damage to cell membranes as a causative factor. In addition to loss of nicotinamide, activation of enzymes by disruption of lysosomes appears to take place in irradiated cervical cancer tissue (Goldberg et al, 1967) and in exfoliated cancer cells obtained by vaginal irrigation (Goldberg et al, 1969;Goldberg, 1971). Leakage of pyruvate (Dose & Dose, 1962) and of K+ (Flemming et al, 1968) also indicate altered permeability of the cell, which must have profound consequences for its metabolic integrity.…”