Effect of reconstituted basement membrane components on the growth of a panel of human tumour cell lines in nude mice

Abstract

Sunmmary Previous reports have indicated that reconstituted basement membrane (matrigel), when co-injected with either established or primary human tumour cells, can improve the growth of subcutaneous xenografts in nude mice. The human adenocarcinoma cell lines A549, SW480, and WiDr, and the human fibrosarcoma cell line HT1080scc2 exhibit varying degrees of tumourigenicity in nude mice. All these lines showed increased tumourigenicity and/or growth rate, together with a change towards a more differentiated tissue morphology, when co-injected with matrigel into nude mice. Experiments using A549 cell line have indicated that the effect of matrigel is concentration-dependent and that increased growth rate is not maintained when xenografts grown with matrigel are passaged into further mice. These results strongly suggest that increased tumour growth results from the improved growth conditions afforded by matrigel, rather than from the selection of subpopulations of the most tumourigenic cells. Increased growth of intracaecal tumours arising from the co-injection of SW480 cells with matrigel, indicate a possible use for matrigel in the development of more relevant animal models using the orthotopic site. Purified laminin significantly increased the growth of sc tumours resultant from co-injection with either WiDr or A549 cells, whereas collagen IV or laminin with entactin showed no such effect. A role for free laminin in the stimulation of cell growth in the absence of an intact basement membrane is discussed.

Modeling human osteosarcoma in mice through 3AB‐OS cancer stem cell xenografts

“…A number of articles have shown that matrigel can help to establish xenografts from a variety of cell lines [Topley et al, 1993].…”

TCR Mimic Monoclonal Antibody Targets a Specific Peptide/HLA Class I Complex and Significantly Impedes Tumor Growth In Vivo Using Breast Cancer Models

“…Matrigel is a mixture of laminin, collagen IV, heparan sulfate, and entactin that creates a nutrientrich environment to promote rapid tumor growth, resulting in palpable tumors within 4-5 wk after cell implantation (48,49). Tumors were allowed to grow to a mean volume of approximately $50 mm 3 before initiation of treatment with RL4B TCRm or isotype control Ab (Fig.…”

Overexpression of laminin ?1 chain in colonic cancer cells induces an increase in tumor growth

“…In particular, it was shown that coinjection of purified laminin-1 and of lung or colon cancer cells increases the growth of the tumors developed as xenografts in nude mice; such an effect was not observed when collagen IV, another basement membrane molecule, was used. 17,18 It was postulated that the increase in tumor growth resulted from the improved growth conditions brought by laminin-1, 17 while other authors showed that the interaction of the laminin-containing Matrigel with human colon adenocarcinoma cells allows the selection of a subset of neoplastic cells that display tumorigenic potential by themselves even in the absence of Matrigel. 19 The mechanisms through which laminin-1 exerts its effect remain unclear.…”