Effect of red propolis on hamster cheek pouch angiogenesis in a new sponge implant model

Abstract

Effect of red propolis on hamster cheek pouch angiogenesis in a new sponge implant model 1 Abstract Purpose: To evaluate the effects of red propolis on cheek pouch angiogenesis in a hamster new model sponge implant. Methods: Forty eight animals divided into eight groups. (Groups I-IV), the animals were treated for 15 days before and 10 days after sponge implantation. (Groups V-VIII), the animals were treated for 10 days after sponge implantation (GI and GV: red propolis 100 mg/kg, GII and GVI: celecoxib 20 mg/kg, GIII and GVII: 1% gum arabic 5 mL/kg, GIV and GVIII: distilled water 5 mL/kg). On the 11th day of implantation, the animals were anesthetized for stereoscopic microscopic imaging and morphometric quantification of angiogenesis (SQAN), followed by histopathological evaluation (H&E). Results: In the SQAN analysis, no significant difference was found between the groups. However, on histology, propolis was found reduce the population of mastocytes in the qualitative analyses (p = 0,013) in the quantitative analyses to reduce the number of blood vessels (p = 0,007), and increase the macrophage count (p = 0,001). Conclusion: Red propolis inhibited inflammatory angiogenesis when administered before andcontinuously after sponge implant, and was shown to have immunomodulating effects on inflammatory cells (mastocytes and macrophages) in a new sponge implant hamster model.

Red propolis reduces inflammation in cyclophosphamide-induced hemorrhagic cystitis in rats

“…In earlier experiments on animal models conducted by our team, red propolis inhibited inflammatory and neoplastic angiogenesis associated with a reduction in inflammatory cells (e.g., mastocytes and macrophages) suggestive of immunomodulatory action 26 , 27 .…”

Therapeutic potential of propolis in alleviating inflammatory response and promoting wound healing in skin burn

“…The findings on propolis' actions regarding neovascularization and angiogenesis have shown contradicting results. In some studies, propolis positively affects angiogenesis (Abdel-Wahed et al, 2013;Nindra et al, 2021;Staniczek et al, 2021;Sungkar et al, 2021), and in other studies it negatively affects angiogenesis (Chikaraishi et al, 2010;Lima et al, 2014;Melo et al, 2018). Sungkar et al (2021) reported that EEP from Mount Lawu, Indonesia has angiogenic effects or promotes angiogenesis through decreased malondialdehyde blood levels and IL-6 blood levels, and an increase in vascular endothelial growth factor (VEGF) blood levels.…”

“…The findings on propolis' actions regarding neovascularization and angiogenesis have shown contradicting results. In some studies, propolis positively affects angiogenesis (Abdel‐Wahed et al, 2013; Nindra et al, 2021; Staniczek et al, 2021; Sungkar et al, 2021), and in other studies it negatively affects angiogenesis (Chikaraishi et al, 2010; Lima et al, 2014; Melo et al, 2018).…”

“…Research by Lima et al (2014) showed that propolis treatment on the wound healing of mice with chronic inflammation (induced by polyether‐polyurethane sponge discs) showed effects of downregulation of angiogenesis and fibrosis, through the decrease of TGF‐β1 and collagen disposition. Melo et al (2018) observed that propolis inhibited inflammatory angiogenesis in a new sponge implant hamster model, where it reduced the number of blood vessels. These results truly show the modulatory properties of propolis and its contents in different processes throughout the healing of wounds.…”

Red propolis and L-lysine on angiogenesis and tumor growth in a new model of hamster cheek pouch inoculated with Walker 256 tumor cells

“…Complement, lymphocyte and macrophage activation has been observed in many studies, suggesting they are part of the mechanism by which propolis induces apoptosis in tumor cells. ( 11 – 13 ) In this way, we therefore included an experiment with 33 days of treatment with propolis prior to inoculation (experiment 2).…”