Effect of resynchronization with GnRH or progesterone (P4) intravaginal device (CIDR) on Day 23 after timed artificial insemination on cumulative pregnancy and embryonic losses in CIDR-GnRH synchronized Nili-Ravi buffaloes

Arshad, Usman; Qayyum, Arslan; Hassan, Mubbashar; Husnain, A.; Sattar, Abdul; Ahmad, Nasim

doi:10.1016/j.theriogenology.2017.07.054

Cited by 17 publications

(9 citation statements)

References 43 publications

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“…In order to circumvent this, double inseminations, with 12 hour interval, after initiation of estrus, were adopted in buffalo (Neglia et al, 2003;Naseer et al, 2011). Pregnancy rate were reported to be fairly high (upto 60 %) in synchronized and (upto 80%) in resynchronized buffalo with fix time inseminations (de Araujo Berber et al, 2001;Baruselli et al, 2001;Warriach et al, 2008;Salzano et al, 2017;Arshad et al, 2017). The higher pregnancy rate, achieved at 24 hours with single AI in this study, is perhaps due to artificial insemination time with regard to timing of ovulation.…”

Section: Discussionmentioning

confidence: 99%

Effect of timing of artificial insemination in relation to onset of standing estrus on pregnancy per AI in Nili-Ravi buffalo

Riaz¹,

Hassan²,

Husnain³

et al. 2018

Anim Reprod

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The aim of the present study was to determine the optimum time of artificial insemination after the beginning of standing estrus in buffalo. Nili-Ravi buffalo (n = 109) during breeding season were exposed to teaser bull at 12 hours interval to determine the standing heat (0 h). Buffalo were randomly allocated to different time groups and a single artificial insemination was performed either at 0 h (n = 30), 12 h (n = 27), 24 h (n = 28) or 36 h (n = 24). In a subset of buffalo (n = 38) ultrasonography was performed, twice daily from 0 h (onset of standing heat) to determine the time of ovulation. Pregnancy diagnosis was performed 35-40 days after AI. Results revealed that mean time of ovulation from onset of standing heat was 34.7 ± 0.96 h (range 30 to 42 h). Higher (P < 0.05) pregnancy per AI were achieved in buffalo when inseminated at 24 h (15/28; 53%) compared to 0 h (8/30; 26%) and 36 h (3/24; 13%). Pregnancy per AI, was in-between, in buffalo, inseminated at 12 h (10/27; 37%) and did not differ (P > 0.05) with those bred either at 24 h or 0 h. The odds ratio further confirmed that the occurrence of pregnancy per AI was two times higher in buffalo inseminated at 24 h as compared to those at 12 h. It is concluded that optimal pregnancy per AI can be achieved when buffalo are bred artificially 24 h after the onset of standing heat.

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Section: Discussionmentioning

confidence: 99%

Effect of timing of artificial insemination in relation to onset of standing estrus on pregnancy per AI in Nili-Ravi buffalo

Riaz¹,

Hassan²,

Husnain³

et al. 2018

Anim Reprod

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“…The establishment of pregnancy requires a progressive increase in progesterone secretion during the first 2–3 weeks after mating. In buffalo, different treatments prolonging the CL lifespan, and thus, supporting pregnancy, have been utilized [ 38 , 55 , 56 ]. Campanile et al [ 38 ], reported that the GnRH treatment on day 5 after AI increases progesterone secretion and the chance of successful pregnancy in buffaloes that mate during periods of increasing daylight length (outside the breeding season) when progesterone secretion is relatively low and the incidence of embryonic mortality tends to be high.…”

Section: Discussionmentioning

confidence: 99%

“…The same researchers, applying a delayed treatment on day 25 after AI, had an increased pregnancy rate [ 57 ]. In Pakistan, Arshad et al [ 56 ] also found that the administration of GnRH at day 23 as a resynchronization strategy in buffalo enhanced the pregnancy rate on day 30 and reduced the cumulative embryonic/fetal losses on days 45, 60 or 90 post the first AI when compared with control buffaloes.…”

Section: Discussionmentioning

confidence: 99%

Use of GnRH Treatment Based on Pregnancy-Associated Glyco-Proteins (PAGs) Levels as a Strategy for the Maintenance of Pregnancy in Buffalo Cows: A Field Study

Pacelli

Barile

Sabia

et al. 2022

Animals

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The aim of the study was to evaluate the effects of GnRH administrated at day 35 after artificial insemination (AI) on the reproductive performance of buffalo cows. In ten buffalo farms in the period January–February, 481 buffalo cows were subjected to estrus synchronization protocol and fixed-time artificial insemination (Ovsynch–TAI program). Radioimmunoassays (RIA) for pregnancy-associated glycoproteins (PAGs) were used to detect pregnancy at day 28 after AI (cut-off value: ≥1 ng/mL). Among pregnant subjects, those with PAG values between 1 and 2.5 ng/mL were considered at risk of embryonic mortality (EM) and were assigned into two groups: treated (T; n = 57) control (C; n = 57). Treated buffaloes received 0.01 mg of buserelin acetate intramuscularly on day 35 after AI, whereas control buffaloes received no treatment. The pregnancy diagnosis was confirmed at day 60 through PAGs level and rectal palpation. The treatment with GnRH had a significant effect (p < 0.001) in reducing EM. Between days 28 and 60 after AI, the animals that experienced EM were only 2/57 in the T group, while were 13/57 in the C group. Moreover, GnRH treatment produced a significant increase (p < 0.001) in the PAG concentration between day 28 and day 60. Administration of GnRH at day 35 after AI in animals considered at risk of low embryo survival based on PAG levels allowed a reduction in pregnancy losses and improved the pregnancy rate during low-breeding season in buffalo.

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“…GnRH was examined as a protector of pregnancy in buffalo cows, related to a suboptimally functioning corpora lutea. Its administration in the various stages of pregnancy, usually after day 25, improved final pregnancy rates [ 94 , 95 ], probably by decreasing early embryonic loss [ 96 ]. The role of GnRH in improving pregnancy rates is an ongoing area of research and is of particular interest in some physiologic states, such as in dairy cows that suffer from heat stress-induced early embryonic losses.…”

Section: Application Of Exogenous Gnrh In Controlled Breedingmentioning

confidence: 99%

Application of Exogenous GnRH in Food Animal Production

Uddin

Petrovski

Song

et al. 2023

Animals

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Over several decades, exogenous GnRH and agonists have been employed for controlling reproductive cascades in animals, and treating some reproductive morbidities. The administration of GnRH is used in animals to counter ovarian dysfunction, induce ovulation, and to increase conception and pregnancy rates. GnRH and its agonists are used in the treatment of cystic ovarian degeneration and repeat breeder syndrome. The development of protocols for GnRH administration by intramuscular injection, intramuscular or subcutaneous implants, and intravaginal deposition has empowered their clinical use worldwide. Currently, exogenous GnRH products are a central part of several pre- and post-breeding programs for the enhancement of fertility, including the control of estrous cycles and timing of ovulation, development of fixed-time artificial insemination protocols, improved embryo survival, and the treatment of reproductive morbidity. The aim of the present review is to summarize the application of exogenous GnRH agonists in food animal production.

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Effect of resynchronization with GnRH or progesterone (P4) intravaginal device (CIDR) on Day 23 after timed artificial insemination on cumulative pregnancy and embryonic losses in CIDR-GnRH synchronized Nili-Ravi buffaloes

Cited by 17 publications

References 43 publications

Effect of timing of artificial insemination in relation to onset of standing estrus on pregnancy per AI in Nili-Ravi buffalo

Effect of timing of artificial insemination in relation to onset of standing estrus on pregnancy per AI in Nili-Ravi buffalo

Use of GnRH Treatment Based on Pregnancy-Associated Glyco-Proteins (PAGs) Levels as a Strategy for the Maintenance of Pregnancy in Buffalo Cows: A Field Study

Application of Exogenous GnRH in Food Animal Production

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