Effect of salinity change and exposure time on the egg stages of two abalone species Haliotis discus discus and H. gigantea

Manuel, Albert V.; Tu, Phan Thi Cam; Tsutsui, Naoaki; Yoshimatsu, Takao

doi:10.1007/s12562-019-01366-4

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Under aquaculture conditions, abalone encounters diverse environmental challenges such as salinity fluctuation. Particularly, abalones on farms in the coastal and inner bays could experience prolonged low salinity conditions due to freshwater intrusion [ 3 , 4 ]. Moreover, abalone encounters a sudden drop in seawater salinity during severe summer rainstorms and typhoon events [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals fluid shear stress (FSS) and atherosclerosis pathway as a candidate molecular mechanism of short-term low salinity stress tolerance in abalone

et al. 2022

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Background Transcriptome sequencing is an effective tool to reveal the essential genes and pathways underlying countless biotic and abiotic stress adaptation mechanisms. Although severely challenged by diverse environmental conditions, the Pacific abalone Haliotis discus hannai remains a high-value aquaculture mollusk and a Chinese predominantly cultured abalone species. Salinity is one of such environmental factors whose fluctuation could significantly affect the abalone’s cellular and molecular immune responses and result in high mortality and reduced growth rate during prolonged exposure. Meanwhile, hybrids have shown superiority in tolerating diverse environmental stresses over their purebred counterparts and have gained admiration in the Chinese abalone aquaculture industry. The objective of this study was to investigate the molecular and cellular mechanisms of low salinity adaptation in abalone. Therefore, this study used transcriptome analysis of the gill tissues and flow cytometric analysis of hemolymph of H. discus hannai (DD) and interspecific hybrid H. discus hannai ♀ x H. fulgens ♂ (DF) during low salinity exposure. Also, the survival and growth rate of the species under various salinities were assessed. Results The transcriptome data revealed that the differentially expressed genes (DEGs) were significantly enriched on the fluid shear stress and atherosclerosis (FSS) pathway. Meanwhile, the expression profiles of some essential genes involved in this pathway suggest that abalone significantly up-regulated calmodulin-4 (CaM-4) and heat-shock protein90 (HSP90), and significantly down-regulated tumor necrosis factor (TNF), bone morphogenetic protein-4 (BMP-4), and nuclear factor kappa B (NF-kB). Also, the hybrid DF showed significantly higher and sustained expression of CaM and HSP90, significantly higher phagocytosis, significantly lower hemocyte mortality, and significantly higher survival at low salinity, suggesting a more active molecular and hemocyte-mediated immune response and a more efficient capacity to tolerate low salinity than DD. Conclusions Our study argues that the abalone CaM gene might be necessary to maintain ion equilibrium while HSP90 can offset the adverse changes caused by low salinity, thereby preventing damage to gill epithelial cells (ECs). The data reveal a potential molecular mechanism by which abalone responds to low salinity and confirms that hybridization could be a method for breeding more stress-resilient aquatic species.

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Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals fluid shear stress (FSS) and atherosclerosis pathway as a candidate molecular mechanism of short-term low salinity stress tolerance in abalone

et al. 2022

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“…The higher total energy used to balnce the osmotic pressure, the less reserved energy utilized for growth, movement, and other biological requirements (Amin et al, 2016). The giant abalone Haliotis gigantean can adapt to low salinity in a long period and produce higher survival rate than the disc abalone (H. discus discus) (Manuel et al, 2019). Morash and Alter (2015) stated that larvae are more easily exposed to diseases due to temperature and salinity fluctuations.…”

Section: Introductionmentioning

confidence: 99%

The performance of gold-mouth turban Turbo chrysostomus larvae in different temperature and salinity media

Hamzah

Nirmala

Supriyono

et al. 2021

Jurnal Akuakultur Indonesia

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Suhu dan salinitas merupakan parameter kualitas air yang berperan penting terhadap proses fisiologis siput mata bulan (T. chrysostomus) sehingga berdampak terhadap perkembangan, pertumbuhan dan kelangsungan hidup larva. Tujuan dari penelitian ini yaitu untuk mengevaluasi pengaruh suhu dan salinitas terhadap perkembangan, pertumbuhan dan kelangsungan hidup larva siput mata bulan (T. chrysostomus). Stadia pre-torsion veliger dicapai sekitar 11 jam 36 menit setelah fertilisasi atau sekitar 3 jam setelah trocophor. Stadia post-torsion veliger awal ditandai dengan cangkang yang telah terbentuk sempurna dan pada post-torsion veliger akhir, larva sudah mengembangkan operkulum, kaki, dan propodium. Hasil pengamatan menunjukan bahwa perlakuan A1B3 memberikan waktu pencapaian stadia post-torsion veliger awal dan post-torsion veliger akhir tercepat yaitu masing-masing 19 jam 36 menit dan 22 jam 36 menit setelah pembuahan. Sedangkan perlakuan A1B1 memberikan waktu pencapaian stadia post-torsion veliger awal dan post-torsion veliger akhir terlama yaitu masing-masing 20 jam 30 menit dan 23 jam 25 menit setelah pembuahan. Suhu tidak berpengaruh nyata sedangkan salinitas berpengaruh nyata terhadap laju pertumbuhan harian larva siput mata bulan. Laju pertumbuhan harian tertinggi pada suhu 27±0.5oC (A1) tercatat pada perlakuan B3 dan menunjukan nilai yang tidak berbeda nyata dengan perlakuan B2. Suhu dan salinitas memberikan pengaruh yang signifikan namun interaksi keduanya tidak menunjukan pengaruh yang signifikan terhadap tingkat kelangsungan hidup larva siput mata bulan. Perlakuan A1B3 memberikan persentase tingkat kelangsungan hidup tertinggi dan tidak menunjukan nilai yang berbeda nyata dengan perlakuan A1B2. Parameter kualitas air yang diperoleh masih mendukung performa larva siput mata bulan hingga mencapai stadia juvenil.

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Fluctuations in the heart rate of abalone in response to low salinity stress

Boamah

Shen

et al. 2021

Aquacult Int

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Effect of salinity change and exposure time on the egg stages of two abalone species Haliotis discus discus and H. gigantea

Cited by 3 publications

References 20 publications

Transcriptome analysis reveals fluid shear stress (FSS) and atherosclerosis pathway as a candidate molecular mechanism of short-term low salinity stress tolerance in abalone

Transcriptome analysis reveals fluid shear stress (FSS) and atherosclerosis pathway as a candidate molecular mechanism of short-term low salinity stress tolerance in abalone

The performance of gold-mouth turban Turbo chrysostomus larvae in different temperature and salinity media

Fluctuations in the heart rate of abalone in response to low salinity stress

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