Effect of sampling time and air humidity on the bioefficiency of filter samplers for bioaerosol collection

Zheng, Wenjing; Reponen, Tiina; Grinshpun, Sergey A.; Górny, Rafał L.; Willeke, Klaus

doi:10.1016/s0021-8502(00)00108-7

Cited by 188 publications

(162 citation statements)

References 17 publications

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“…(14) Spores are approximately 0.7 µm in diameter and 1.8 µm in length in the dried state, (15) with an average aerodynamic diameter reported to range from approximately 0.8-1.1 µm. (14,16,17) B. atrophaeus endospores (ATCC #9372) were obtained from Raven Labs (Omaha, Neb.) in a stock solution of 1 × 10 9 colony forming units (CFU) per 0.1 mL suspended in deionized water.…”

Section: B Atrophaeus Bacteriamentioning

confidence: 99%

Performance of Conventional and Antimicrobial-Treated Filtering Facepiece Respirators Challenged with Biological Aerosols

Lore

Sebastian

Brown

et al. 2012

Journal of Occupational and Environmental Hygiene

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Section: B Atrophaeus Bacteriamentioning

confidence: 99%

Performance of Conventional and Antimicrobial-Treated Filtering Facepiece Respirators Challenged with Biological Aerosols

Lore

Sebastian

Brown

et al. 2012

Journal of Occupational and Environmental Hygiene

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“…The collected particles were extracted from the filters using a touch mixer (Model 231, Fisher Scientific, Pittsburgh, PA, USA) that operated for 2 min followed by a 10-min ultrasonic bath (FS20, Fisher Scientific, Pittsburgh, PA, USA) agitation. Our earlier study revealed that a method combining vortexing with ultrasonic agitation exhibited an extraction efficiency of 96-98% when the microorganisms were extracted from polycarbonate filters; at the same time, it did not considerably reduce the culturability of microorganisms, including sensitive bacteria such as Pseudomonas fluorescens (Wang et al, 2001). All the filter preparation and handling procedures were performed in a Class II Biosafety cabinet.…”

Section: Sample Analysismentioning

confidence: 99%

Culturability and concentration of indoor and outdoor airborne fungi in six single-family homes

Lee

Grinshpun

Martuzevičius

et al. 2006

Atmospheric Environment

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136

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In this study, the culturability of indoor and outdoor airborne fungi was determined through longterm sampling (24-h) using a Button Personal Inhalable Aerosol Sampler. The air samples were collected during three seasons in six Cincinnati area homes that were free from moisture damage or visible mold. Cultivation and total microscopic enumeration methods were employed for the sample analysis. The geometric means of indoor and outdoor culturable fungal concentrations were 88 and 102 colony-forming units (CFU) m -3 , respectively, with a geometric mean of the I/O ratio equal to 0.66. Overall, 26 genera of culturable fungi were recovered from the indoor and outdoor samples. For total fungal spores, the indoor and outdoor geometric means were 211 and 605 spores m -3 , respectively, with a geometric mean of I/O ratio equal to 0.32. The identification revealed 37 fungal genera from indoor and outdoor samples based on the total spore analysis. Indoor and outdoor concentrations of culturable and total fungal spores showed significant correlations (r = 0.655, p<0.0001 and r = 0.633, p<0.0001, respectively). The indoor and outdoor median viabilities of fungi were 55% and 25%, respectively, which indicates that indoor environment provides more favorable survival conditions for the aerosolized fungi. Among the seasons, the highest indoor and outdoor culturability of fungi was observed in the fall. Cladosporium had a highest median value of culturability (38% and 33% for indoor and outdoor, respectively) followed by Aspergillus/ Penicillium (9% and 2%) among predominant genera of fungi. Increased culturability of fungi inside the homes may have important implications because of the potential increase in the release of allergens from viable spores and pathogenicity of viable fungi on immunocompromised individuals.

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“…Capillary pore membrane filters have been utilized for sampling and recovery of bacterial aerosols [15,16]. Gelatin filters have been used effectively for sampling airborne bacteria with subsequent digestion of the filters for quantitative analyses of the microorganisms [17].…”

Section: Introductionmentioning

confidence: 99%

“…Respiratory protection filters made from fibrous media have been utilized to capture experimentally generated bacterial aerosols [18,19]. One of the barriers noted to the use of filters for sampling bioaerosols is the potential loss of culturability and/or viability of the organisms as the sampling period progresses [16].…”

Section: Introductionmentioning

confidence: 99%

“…This has been accomplished by immersing small filters or portions of filters in a solution of a Tween surfactant in water and agitating by one of the several methods for a suitable time [16,18,19,25,27,28]. Forms of agitation evaluated have included manual shaking [18,28], mechanical shaking [18,19,25], blending [18,27], vortexing [16,19], and ultrasonic vibration [16,18,19].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimizing the Recovery of Surrogates for Bacterial Bioterrorism Agents from Ventilation Filters

Kim

Raynor²,

Kuehn

et al. 2008

CLEAN Soil Air Water

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Heating, ventilating, and air-conditioning (HVAC) filters have the potential to serve as long-term integrated samplers for biological particles that may be released intentionally or unintentionally into a public building. Methods for eluting bacteria that are surrogates for potential bioterrorism agents from HVAC filters were tested for their ability to recover as high a percentage of culturable bacteria as possible. A solution of 0.02% Tween-80 in water was an effective eluent for Bacillus globigii (BG) spores whereas phosphate-buffered saline was the most effective eluent for vegetative bacteria. Hand shaking was a more effective agitation method for high recovery rates than vortexing or mechanical shaking. The results indicate that BG spores can be eluted successfully while maintaining essentially 100% culturability. The recovery of the vegetative bacteria Mannheimia haemolytica and Yersinia ruckeri was 80% or higher under optimal conditions. The culturability of the vegetative bacteria eluted from HVAC filters declined substantially within as little as 2 h after the bacteria were loaded onto the filter. The culturability of the BG spores eluted from filters exhibited an exponential decay subsequent to loading with a half-life of 8 -9 days.

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Effect of sampling time and air humidity on the bioefficiency of filter samplers for bioaerosol collection

Cited by 188 publications

References 17 publications

Performance of Conventional and Antimicrobial-Treated Filtering Facepiece Respirators Challenged with Biological Aerosols

Performance of Conventional and Antimicrobial-Treated Filtering Facepiece Respirators Challenged with Biological Aerosols

Culturability and concentration of indoor and outdoor airborne fungi in six single-family homes

Optimizing the Recovery of Surrogates for Bacterial Bioterrorism Agents from Ventilation Filters

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