Effect of Sargassum serratifolium Extracts on β-Amyloid Production

Choi, Min-Woo; Jung, Cha-Gyun; Kim, Hyeung‐Rak

doi:10.5657/kfas.2017.0085

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Heo et al [ 80 ] found that fucoxanthin isolated from S. siliquastrum at concentrations of 5–200 µM reduced the toxicity induced by H 2 O 2 in Vero cells, while Alghazwi et al [ 79 ] suggested that pretreatment with fucoxanthin (0.1–2 µM) increased the survival rate of cells damaged by Aβ 25–35 up to 67–98.5%. Choi et al [ 81 ] also indicated that an alcohol extract of S. serratifoilum reduced the accumulation of Aβ 1–42 in Chinese hamster ovary cells (CHO-751 cells). Our results ( Figure 3 and Figure 4 ) correspond with the statement that concentrations of the tested sample likely depend on cell lines, and some cell lines, such as PC-12 cells, are more sensitive than others.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Fucoxanthin from Sargassum oligocystum Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity

Hong,

Thom,

et al. 2023

Biomedicines

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Fucoxanthin extracted and purified from Vietnamese Sargassum oligocystum Montagne, 1845 exhibits various biological activities. In this study, the ability of fucoxanthin to inhibit acetylcholinesterase (AChE), the antioxidant activities, and the expression of antioxidant enzymes were investigated. Fucoxanthin isolated from Vietnamese S. oligocystum showed no cytotoxic effects; moreover, it exhibited AChE inhibitory activity (with an IC50 value of 130.12 ± 6.65 μg mL−1) and antioxidant activity (with an IC50 value of 3.42 ± 0.15 mg mL−1). At concentrations of 50 and 100 µg mL−1, fucoxanthin provided protection against amyloid β-protein fragment 25–35-induced neurotoxicity in a C6 neuronal cell line, and the survival of C6 cells was higher than 81.01% and 80.98%, respectively, compared to the control group (59%). Moreover, antioxidant enzyme activity and quantitative PCR analysis suggested that the neuroprotective effect of fucoxanthin resulted from regulation of the gene expression of antioxidant enzymes (CAT and GPx) and ER pathways (caspase-3 and Bax), as well as the promotion of expression of genes involved in PI3K/Akt signaling (GSK-3β), autophagy (p62 and ATG5), and the biosynthesis of ACh (VAChT and ChAT). Therefore, fucoxanthin extracted from the seaweed S. oligocystum in Vietnam is a potential feedstock source for the production of health foods that exert neuroprotective effects.

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Section: Discussionmentioning

confidence: 99%

Isolation of Fucoxanthin from Sargassum oligocystum Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity

Hong,

Thom,

et al. 2023

Biomedicines

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“…The structure of fucoxanthin was determined by nuclear magnetic resonance (NMR) spectra recorded on a Bruker Avance Neo 600 MHz spectrometer with CDCl 3 as solvent and TMS as internal standard. 1 H NMR spectra was measured at 600 MHz and 13 C was measured at 150 MHz at the Institute of Chemistry, Vietnam Academy of Science and Technology. The structure of fucoxanthin was determined by comparing the NMR data with the published standard (Yan et al 1999).…”

Section: Determination Of Fucoxanthin Structurementioning

confidence: 99%

Elucidation of Antioxidant and Neuroprotective Potential of Fucoxanthin Isolated from Brown Seaweed Sargassum oligocystum

Thom

Thu

et al. 2023

Preprint

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Fucoxanthin belonging to the carotenoid family has different bioactive properties as anti-oxidant, anti-obesity, anti-diabetic, and anti-cancer effects. This study presents the results of the extraction and purification of fucoxanthin from Sargassum olygocystum Montagne, 1845 using thin-layer chromatography, column chromatography, and high-performance liquid chromatography. Furthermore, this study demonstrated the antioxidant activities, expression of antioxidant enzymes, and inhibition of acetylcholinesterase (AChE) of fucoxanthin. The chemical structure, molecular formula (C42H58O6), and molecular weight (658) of fucoxanthin were determined by nuclear magnetic resonance spectroscopy. Fucoxanthin isolated from S. olygocystum showed no cytotoxic effects, moreover, it showed potent antioxidant activity as assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method (with an IC50 value of 3.42 ± 0.15 mg mL− 1), and AchE inhibitory activity (with an IC50 value of 130.12 ± 6.65 µg mL− 1). At concentrations of 50 and 100 µg mL− 1, fucoxanthin protected against amyloid β-protein fragment 25–35 - induced neurotoxicity on the C6 neuronal cell line with the survival of C6 cells higher than 81.01 and 80.98%, respectively, compared to the control group (59%). Moreover, antioxidant enzyme activity and quantitative PCR analysis revealed that the neuroprotective effect of fucoxanthin, was possibly due to not only regulation of gene expression of antioxidant enzyme (CAT and GPx), ER pathway (caspase-3 and Bax) but also promoting expression of genes involved in PI3K/Akt signaling (GSK-3β), autophagy (p62 and ATG5) and the biosynthesis of ACh (VAChT and ChAT). Therefore, fucoxanthin extracted from the Sargassum olygocystum is a potential feedstock source for the production of health foods with neuroprotective effects.

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“…Fucosterol from Padina australis was evaluated for its neuroprotective effects in SH-SY5Y cells treated with Aβ 25-35 ; activation of the down-regulation of amyloid precursor protein (APP) expression (Alghazwi et al, 2018). Choi et al (2017) also reported that ethanol extract of S. serratifoilum reduced the accumulation of β-amyloid (Aβ 1-42 ) in the cell line CHO-751 (Chinese hamster ovary cells). Our obtained results were also similar to the above statement.…”

Section: Protective Activity Against Cytotoxicity In Alzheimer's Dise...mentioning

confidence: 99%

Screening of neuroprotective substances relevant to Alzheimer’s disease from seaweed species collected in Ninh Thuan and Khanh Hoa provinces, Vietnam

Tam

Hien

Thom

et al. 2022

AJB

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Currently, nearly 36.5 million people live with Alzheimer’s disease worldwide, and acetylcholinesterase inhibition is considered the main treatment strategy against it. Seaweeds (or macroalgae) are a natural source of high-value bioactive compounds and have great potential in the production of health foods/foods, pharmaceuticals, cosmetics, animal feeds, biofertilizers, and biofuels. The most studied and applied seaweed species include red seaweed (Rhodophyta), green seaweed (Chlorophyta) and brown seaweed (Phaeophyta). This study aimed to evaluate the antioxidant and neuroprotective activities of extracts/substances isolated with solvents including ethanol 75o and 96o, water, n-hexane, ethyl acetate under normal and ultrasonic conditions (power 80W, frequency 4.7 kHz and for 1 hour) from 5 economically important seaweed species belonging to genera Caulerpa, Sargassum, Gracilaria, Ulva and Kappaphycus collected in Ninh Thuan and Khanh Hoa provinces, Vietnam. The results have shown that 05/30 extracts and substances (including SaE96S, SaH, SaHW, SaEA and fucosterol) isolated from five seaweed species exhibited high antioxidant activity through the free radical screening method using 2,2-diphenyl-1-picrylhydrazyl (DPPH) (with EC50 values < 5 mg/mL for extracts and EC50 value < 2 mM for substance) in comparison with positive control ascorbic acid (EC50 = 0.015 mg/mL); acetylcholinesterase inhibitory activity using acetylcholinesterase inhibitor screening KIT (with IC50 values of < 200 µg/mL for both extracts and substances), compared with positive control galantamine (with IC50 value of 52,8 µg/mL). The extracts/substances were able to protect the cell against cytotoxity in the C6 alzheimer’s disease cell model induced by amyloid beta-protein fragment (Aβ25-35). The results achieved from this research have proven that the extracts/substances isolated from seaweed species were a potential source of medicinal agents for the prevention and treatment of alzheimer’s disease.

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Effect of Sargassum serratifolium Extracts on β-Amyloid Production

Cited by 4 publications

References 31 publications

Isolation of Fucoxanthin from Sargassum oligocystum Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity

Isolation of Fucoxanthin from Sargassum oligocystum Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity

Elucidation of Antioxidant and Neuroprotective Potential of Fucoxanthin Isolated from Brown Seaweed Sargassum oligocystum

Screening of neuroprotective substances relevant to Alzheimer’s disease from seaweed species collected in Ninh Thuan and Khanh Hoa provinces, Vietnam

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